Objective — to develop a universal procedure for measuring vaginal pH values, by using a current laboratory pH meter and by studying the pH values in various vaginal topographic areas in normal biocenosis and bacterial vaginosis for the early diagnosis and prevention of latent and chronic forms of this disease. Subject and methods. A total of 1064 women aged 20 to 35 years were examined; a control group consisted of 331 patients with normal biocenosis; a study group included 558 patients with the clinical signs and laboratory evidence of bacterial vaginosis; a recurrence risk group comprised 175 patients in whom bacterial vaginosis was confirmed only by polymerase chain reaction results without clinical manifestations of the disease. Standard clinical and laboratory examination was conducted. The procedure developed by the authors for the comparative hardware topographic hardware pH-metry of vaginal contents was applied. The pH values of the vagina were measured in 6 points of its vaults by means of the pH meter — an AGM-MP-03-1 microprocessor acidogastrometer, and an endoscopic pH-probe on days 21—23 of the menstrual cycle. A spatial vaginal pH chart was made for each patient, which was compared with the spatial chart pattern in normal biocenosis through their mutual overlay. Results. The procedure allows one to identify areas of dysbiotic disease states that are manifested by the shifting of boundaries and by the increase of the area of these states. The determination of the direction of displacement of the spatial vaginal pH chart makes it possible to estimate the areas of lesions and the location of formed bacterial films, subsequently, to effectively sanitize the vagina, and to prevent recurrent bacterial vaginosis. Conclusion. A large clinical material has shown that it is expedient to use hardware topographic pH-metry of the vaginal environment as a quick and easy technique to diagnose bacterial vaginosis in an outpatient setting.