Endometrial omix marker in patients with endometriosis and pelvic pain: conjugated ubiquitin

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BACKGROUND

To important and unexplored objects in endometriosis should be considered the role of ubiquitin and ubiquitination. Since the discovery of ubiquitin, there has been a lot of interest in this small but highly abundant protein in eukaryotic cells. Excessive accumulation of ubiquitinated protein in cells can lead to dysfunction and pathological process.

OBJECTIVES

To study the role of conjugated and unconjugated ubiquitin in eutopic and ectopic endometrium in patients with peritoneal endometriosis using Western blotting, immunohistochemical analysis, quantitative polymerase chain reaction in real time, enzyme immunoassay in blood and peritoneal fluid and to compare these data with the presence of chronic pelvic pain.

METHOD

Western blotting was used to quantify the content of conjugated and unconjugated ubiquitin in eutopic and ectopic endometrium in women with endometriosis. Immunohistochemical analysis was used to compare the results of ubiquitin expression in various cellular structures. Real-time quantitative polymerase chain reaction was used to assess ubiquitin gene expression. Determination of the concentration of ubiquitin in the peritoneal fluid and in the blood was carried out by enzyme immunoassay. All results were compared according to the presence or absence of chronic pelvic pain in relation to the control group.

RESULTS

An increase in the content of conjugated ubiquitin in the tissue of eutopic and ectopic endometrium in the presence of pain was proved, as well as an increase in the level of ubiquitin not conjugated to the protein, according to Western blotting analysis. The ratios between these proteins showed the greatest contribution of protein-conjugated ubiquitin rather than free ubiquitin. The developed Western blot analysis method for the identification of conjugated ubiquitin in the tissue of eutopic and ectopic endometrium can be used to assess the expression of the ubiquitin protein as an omix marker of impairment of the ubiquitin-proteosomal system and proteasome activity.

CONCLUSIONS

Ubiquitin conjugated to proteins is a universal omix marker involved in the accumulation of ubiquitinated proteins for their subsequent degradation. The accumulation of ubiquitinated proteins indicates a disturbance in the eutopic and ectopic endometrium of the ubiquitin-proteasomal system. The results obtained can be used to assess the state of the ubiquitin-proteasomal system in endometriosis.

Keywords:

endometriosis

endometrium

pain

eutopic and ectopic endometrium

conjugated ubiquitin

unconjugated ubiquitin

protein degradation

Authors:

Burlev V.A.

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation Federal;
Uppsala University

SPIN RSCI: 2979-0175
Scopus AuthorID: 12763523100
ORCID: 0000-0002-5271-2478

Received:

15.04.2021

Accepted:

16.05.2022

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