Proteostasis and decreased alphaB-crystallin activity in eutopic and ectopic endometrium in patients with endometriosis and chronic pelvic pain

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BACKGROUND

Proteostasis in human cells is essential for organizing a complex and ramified network of thousands of proteins that interact together to ensure that the proteome functions correctly and efficiently. A decrease in the efficiency of proteostasis due to a decrease in the functional activity of the alphaB-crystallin chaperone in endometriosis can contribute to a change in conformation due to partial unfolding of proteins, which can lead to the formation of a protein with an irregular structure and its aggregation. The undesirable consequences of such changes include the loss of protein function and/or the formation of toxic products.

OBJECTIVES

To study the activity of the alphaB-crystallin chaperone in the eutopic and ectopic endometrium in patients with peritoneal endometriosis using immunohistochemical analysis, Western blotting, quantitative polymerase chain reaction in real time, enzyme immunoassay in blood and peritoneal fluid and to compare these data depending on the presence of chronic pelvic pain.

METHOD

Immunohistochemical analysis, Western blotting, and real-time quantitative polymerase chain reaction were used to investigate the expression of the alphaB-crystallin chaperone in eutopic and ectopic endometrium in women with endometriosis. The ELISA method was used to determine the concentration of alphaB-crystallin in the peritoneal fluid and in the blood serum. All analyzes were used to assess depending on the presence or absence of chronic pelvic or in relation to the control group.

RESULTS

A decrease in the activity of the alphaB-crystallin chaperone in the eutopic and ectopic endometrium in patients with endometriosis and chronic pelvic pain was found, with a simultaneous increase in its content in the peritoneal fluid and in the blood. The effect of the alphaB-crystallin chaperone on proteins provides an opportunity to use this indicator in the diagnosis of chronic stress and in the treatment or prevention of conditions dependent on protein aggregation in functionally active cell structures.

CONCLUSION

Determination of the activity of the alphaB-crystallin chaperone in patients with endometriosis and chronic pelvic pain showed the presence of proteostasis dysregulation. These data can be used to characterize chronic stress in patients with endometriosis and pain.

Keywords:

endometrium

pain

eutopic and ectopic endometrium

alphaB-crystallin

proteostasis

Authors:

Burlev V.A.

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation Federal;
Uppsala University

SPIN RSCI: 2979-0175
Scopus AuthorID: 12763523100
ORCID: 0000-0002-5271-2478

Ilyasova N.A.

Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology;
Women’s and Children’s Health Rudbeck laboratory of the Uppsala University

SPIN RSCI: 8780-3482
Scopus AuthorID: 56507187500
ORCID: 0000-0003-0665-3515

Received:

23.03.2021

Accepted:

18.10.2021

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