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Danilova N.V.

FGBU "Moskovskiĭ nauchno-issledovatel'skiĭ onkologicheskiĭ institut im. P.A. Gertsena" Minzdrava RF;
GBOU DPO "Rossiĭskaia meditsinskaia akademiia poslediplomnogo obrazovaniia", Moskva

Andreeva Yu.Yu.

Russian Medical Academy of Continuing Professional Education, Ministry of Health of Russia

Zavalishina L.É.

FGBU "Moskovskiĭ nauchno-issledovatel'skiĭ onkologicheskiĭ institut im. P.A. Gertsena" Minzdravsotsrazvitiia Rossii

Mal'kov P.G.

GUNU "Fakul'tet fundamental'noĭ meditsiny MGU im. M.V. Lomonosova";
GBOU DPO "Rossiĭskaia meditsinskaia akademiia poslediplomnogo obrazovaniia", Moskva

Apoptotic markers and cell cycle regulatory proteins during background and precancerous changes in the glandular epithelium of the cervix uteri

Authors:

Danilova N.V., Andreeva Yu.Yu., Zavalishina L.É., Mal'kov P.G.

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Journal: P.A. Herzen Journal of Oncology. 2013;2(1): 12‑16

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To cite this article:

Danilova NV, Andreeva YuYu, Zavalishina LÉ, Mal'kov PG. Apoptotic markers and cell cycle regulatory proteins during background and precancerous changes in the glandular epithelium of the cervix uteri. P.A. Herzen Journal of Oncology. 2013;2(1):12‑16. (In Russ.)

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Investigations using the markers p16, Ki-67, p53, and p27 were conducted to specify the existing classification and to define markers for the differential diagnosis of precancerous and benign background changes. Specimens from 118 patients with cervical adenocarcinoma showing different concurrences of benign nontumor and precancerous changes were examined. Immunohistochemical studies using antibodies to p16, p53, p27, and Ki-67 were performed according to the standard protocol, by making a semiquantitative assessment of their results. The expression of p16 and Ki-67 was found to be significantly higher in grade III glandular epithelial dysplasia and adenocarcinoma in situ than in grades I-II dysplasia. There were great differences in the levels of p16 and Ki-67 between grades I-II dysplasia and benign background changes. There was a trend towards increases in the expression of p53 and p27 proteins and in the grade of dysplasia. It is logical to simplify the classification of precancerous changes, by uniting grade III glandular epithelial dysplasia and adenocarcinoma in situ, as well as grades I-II dysplasia into one category.

Keywords:

cervix uteri
immunohistochemistry
precancerous changes

Authors:

Danilova N.V.

FGBU "Moskovskiĭ nauchno-issledovatel'skiĭ onkologicheskiĭ institut im. P.A. Gertsena" Minzdrava RF;
GBOU DPO "Rossiĭskaia meditsinskaia akademiia poslediplomnogo obrazovaniia", Moskva

Andreeva Yu.Yu.

Russian Medical Academy of Continuing Professional Education, Ministry of Health of Russia

Zavalishina L.É.

FGBU "Moskovskiĭ nauchno-issledovatel'skiĭ onkologicheskiĭ institut im. P.A. Gertsena" Minzdravsotsrazvitiia Rossii

Mal'kov P.G.

GUNU "Fakul'tet fundamental'noĭ meditsiny MGU im. M.V. Lomonosova";
GBOU DPO "Rossiĭskaia meditsinskaia akademiia poslediplomnogo obrazovaniia", Moskva

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