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Permikin Zh.V.

Regional Children’s Hospital;
Ural State Medical University;
Research Institute of Medical Cell Technologies

Tsaur G.A.

Regional Children’s Hospital;
Ural State Medical University;
Research Institute of Medical Cell Technologies

Verzhbitskaya T.Yu.

Regional Children’s Hospital;
Research Institute of Medical Cell Technologies

Riger T.O.

Regional Children’s Hospital;
Research Institute of Medical Cell Technologies

Nokhrina E.S.

Regional Children’s Hospital

Plekhanova O.M.

Regional Children’s Hospital

Saveliev L.I.

Regional Children’s Hospital;
Ural State Medical University;
Research Institute of Medical Cell Technologies

Kovtun O.P.

Ural State Medical University

Tsvirenko S.V.

Ural State Medical University

Fechina L.G.

Regional Children’s Hospital;
Ural State Medical University

Popov A.M.

Dmitry Rogachev National Medical Research Center for Pediatric Hematology, Oncology and Immunology, Moscow, Russian Federation

Application of CD27 AND CD44 expression for prediction of ETV6::RUNX1 fusion gene presence in pediatric B-CELL precursor acute lymphoblastic leukemia

Authors:

Permikin Zh.V., Tsaur G.A., Verzhbitskaya T.Yu., Riger T.O., Nokhrina E.S., Plekhanova O.M., Saveliev L.I., Kovtun O.P., Tsvirenko S.V., Fechina L.G., Popov A.M.

More about the authors

Journal: Laboratory Service. 2022;11(4): 21‑30

DOI: 10.17116/labs20221104121

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Table of contents

APPLICATION OF CD27 AND CD44 EXPRESSION FOR PREDICTION OF ETV6::RUNX1 FUSION GENE PRESENCE IN PEDIATRIC B-CELL PRECURSOR ACUTE LYMPHOBLASTIC LEUKEMIA
APPLICATION OF CD27 AND CD44 EXPRESSION FOR PREDICTION OF ETV6::RUNX1 FUSION GENE PRESENCE IN PEDIATRIC B-CELL PRECURSOR ACUTE LYMPHOBLASTIC LEUKEMIA

To cite this article:

Permikin ZhV, Tsaur GA, Verzhbitskaya TYu, et al. . Application of CD27 AND CD44 expression for prediction of ETV6::RUNX1 fusion gene presence in pediatric B-CELL precursor acute lymphoblastic leukemia. Laboratory Service. 2022;11(4):21‑30. (In Russ.)
https://doi.org/10.17116/labs20221104121

Подписка 2025 Лабораторная служба (Laboratory Service)
МСФ на ЯМ
Использование инфографики авторами научных работ
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Abstract:

PURPOSE

To assess the possibility of immunophenotyping of initial blast cell population by flow cytometry for the predicting of presence of translocation t(12;21)(p13;q22)/ETV6::RUNX1 by application of CD27 and CD44 antigens in childhood B-cell precursor acute lymphoblastic leukemia (BCP-ALL).

METHODS

437 patients with childhood BCP-ALL were included in the current analysis. Percentage of CD27- and CD44-positive leukemic cells together with mean fluorescence intensity (MFI) were compared with results of reverse-transcriptase PCR and/or FISH for translocation t(12;21)(p13;q22)/ETV6::RUNX1 detection. Receiver operator characteristic curves was used to find optimal threshold levels for CD27 and CD44 positivity.

RESULTS

Translocation t(12;21)(p13;q22)/ETV6::RUNX1 was associated with combination of positive CD27 and diminished or absent CD44 expression. Although, optimal percentage threshold levels for CD27 and CD44 were determined as 80.7% and 86.1%, respectively. Ratio of MFI CD27 to MFI CD44 showed highest diagnostic odds ratio — 953.1.

CONCLUSION

Flow cytometric detection of CD27 and CD44 was able to predict presence of translocation t(12;21)(p13;q22) / ETV6::RUNX.

Keywords:

t(12;21)(p13;q22)
ETV6::RUNX1
translocation
chimeric gene
flow cytometry
acute lymphoblastic leukemia
children
immunophenotyping

Authors:

Permikin Zh.V.

Regional Children’s Hospital;
Ural State Medical University;
Research Institute of Medical Cell Technologies

Tsaur G.A.

Regional Children’s Hospital;
Ural State Medical University;
Research Institute of Medical Cell Technologies

Verzhbitskaya T.Yu.

Regional Children’s Hospital;
Research Institute of Medical Cell Technologies

Riger T.O.

Regional Children’s Hospital;
Research Institute of Medical Cell Technologies

Nokhrina E.S.

Regional Children’s Hospital

Plekhanova O.M.

Regional Children’s Hospital

Saveliev L.I.

Regional Children’s Hospital;
Ural State Medical University;
Research Institute of Medical Cell Technologies

Kovtun O.P.

Ural State Medical University

Tsvirenko S.V.

Ural State Medical University

Fechina L.G.

Regional Children’s Hospital;
Ural State Medical University

Popov A.M.

Dmitry Rogachev National Medical Research Center for Pediatric Hematology, Oncology and Immunology, Moscow, Russian Federation

Received:

11.12.2022

Accepted:

30.12.2022

Close metadata

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