Diagnostic and prognostic value of protein markers determination of brain damage in mild traumatic brain injuries

Kovtun N.A.; Savelyeva M.I.; Trophimenko A.V.; Alexanin S.S.; Boyarincev V.V.

doi:https://doi.org/10.17116/labs20211002128

Kovtun N.A.

Clinical Hospital No. 1 of Department of President Affairs, Moscow

Savelyeva M.I.

Russian Medical Academy of Continuous Professional Education

Trophimenko A.V.

Central State Medical Academy of President Affairs

Alexanin S.S.

Russian Center for Emergency and Radiation Medicine named after A.M. Nikiforov EMERCOM of Russia

Boyarincev V.V.

Central State Medical Academy of President Affairs

Diagnostic and prognostic value of protein markers determination of brain damage in mild traumatic brain injuries

Authors:

Kovtun N.A., Savelyeva M.I., Trophimenko A.V., Alexanin S.S., Boyarincev V.V.

More about the authors

Journal: Laboratory Service. 2021;10(2): 28‑33

DOI: 10.17116/labs20211002128

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To cite this article:

Kovtun NA, Savelyeva MI, Trophimenko AV, Alexanin SS, Boyarincev VV. Diagnostic and prognostic value of protein markers determination of brain damage in mild traumatic brain injuries. Laboratory Service. 2021;10(2):28‑33. (In Russ.)
https://doi.org/10.17116/labs20211002128

Подписка 2025 Лабораторная служба (Laboratory Service)

Использование инфографики авторами научных работ

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Abstract:

Easy traumatic brain injury (TBI) also known as concussion is a traumatic force to the brain leading to disruption of its functioning. Symptomatology associated with a concussion is primarily functional in nature since the standard brain imaging methods do not reveal structural abnormalities; however postmortem analysis of brains of patients who have suffered a moderate head injury recently but deaths from non-traumatic causes showed signs of damage to the axons. Diagnostic and prognostic tools for risk stratification in patients with mild TBI (mTBI) is limited in the early stages after injury. Currently, there is a critical need in testing and implementation of new laboratory diagnostic tools in mild head injury in clinical practice.

THE AIM OF THE STUDY

To investigate biochemical markers of cellular damage to the brain for the development of an algorithm for clinical and diagnostic verification of mild traumatic brain injuries.

MATERIAL AND METHODS

A prospective comparative study included 121 people in the three comparison groups. Measurement of the level of examined biomarkers in the dynamics was carried out using the methods of ELISA. Measurement of the main laboratory indicators of the general condition of the patient was carried out using automatic analyzers. All statistical calculations were performed by using SPSS version 20.0 and GraphPad Prism version 5.0 (GraphPad Inc).

THE STUDY RESULTS

It was found that the determination of the concentration of Tau- (with the exception of Tau3), GFAP- and pNF-proteins in dynamic measurement improves the diagnosis of cellular damage to the brain in mild TBI, regardless of the computer tomography (CT) scan results, and increases the risk of an adverse prognosis. An algorithm for the verification of mild TBI was developed on the basis of the determination of Tau-, GFAP- and pNF-blood proteins in dynamics.

CONCLUSION

The determination of the concentration of Tau-, GFAP-, and pNF-proteins can be considered as diagnostic and prognostic biomarkers of cellular damage to the brain regardless of the CT results.

Keywords:

cellular biomarkers of brain injury

Tau-protein

GFAP protein

pNF-protein

brain traumatic injury

mTBI

Authors:

Kovtun N.A.

Clinical Hospital No. 1 of Department of President Affairs, Moscow

Savelyeva M.I.

Russian Medical Academy of Continuous Professional Education

Trophimenko A.V.

Central State Medical Academy of President Affairs

Alexanin S.S.

Russian Center for Emergency and Radiation Medicine named after A.M. Nikiforov EMERCOM of Russia

Boyarincev V.V.

Central State Medical Academy of President Affairs

Received:

20.02.2021

Accepted:

01.04.2021

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References:

Hyder AA, Wunderlich CA, Puvanachandra P, Gururaj G, Kobusingye OC. The impact of traumatic brain injuries: A global perspective. Neuro Rehabilitation. 2007;22:341-353. https://doi.org/10.3233/NRE-2007-22502
Hoffman SW, Harrison C. The interaction between psychological health and traumatic brain injury: a neuroscience perspective. The Clinical Neuropsychologist. 2009;23(8):1400-1415. https://doi.org/10.1080/13854040903369433
Niogi SN, et al. Extent of microstructural white matter injury in postconcussive syndrome correlates with impaired cognitive reaction time: a 3T diffusion tensor imaging study of mild traumatic brain injury. American Journal of Neuroradiology. 2008;29:5:967-973. https://doi.org/10.3174/ajnr.A0970
Wilde EA, et al. Diffusion tensor imaging of acute mild traumatic brain injury in adolescents. Neurology. 2008;70:12:948-955. https://doi.org/10.1212/01.wnl.0000305961.68029.54
Smits M, et al. Microstructural brain injury in post-concussion syndrome after minor head injury. Neuroradiology. 2011;53:8:553-563. https://doi.org/10.1007/s00234-010-0774-6
Unden J, Ingebrigtsen T, Romner B. Scandinavian guidelines for initial management of minimal, mild and moderate head injuries in adults: An evidence and consensus-based update. BMC Med. 2013;11:50. https://doi.org/10.1186/1741-7015-11-50
Johnson VE, Stewart W, Smith DH. Axonal pathology in traumatic brain injury. Exp Neurol. 2012;246:35-43. https://doi.org/10.1016/j.expneurol.2012.01.013
Hjalmarsson C, Bjerke M, Andersson B, Blennow K, Zetterberg H, Aberg N, Olsson B, Eckerström C, Bookmark L, Wallin A. Neuronal and glia-related biomarkers in cerebrospinal fluid of patients with acute ischemic stroke. J Cent Nerv Syst Dis. 2014;19:6:51-58. https://doi.org/10.4137/JCNSD.S13821
Kochanek PM, Berger RP, Bayr H, Wagner AK, Jenkins LW, Clark RS. Biomarkers of primary and evolving damage in traumatic and ischemic brain injury: Diagnosis, prognosis, probing mechanisms, and therapeutic decision making. Curr Opin Crit Care. 2008;14:135-141. https://doi.org/10.1097/MCC.0b013e3282f57564
Metting Z, Wilczak N, Rodiger LA, Schaaf JM, van der Naalt J. GFAP and S100B in the acute phase of mild traumatic brain injury. Neurology. 2012;78:1428-1433. https://doi.org/10.1212/WNL.0b013e318253d5c7
Shahim P, Tegner Y, Wilson DH, Randall J, Skillback T, Pazooki D, Kallberg B, Blennow K, Zetterberg H. Blood biomarkers for brain injury in concussed professional ice hockey players. JAMA Neurol. 2014;71:684-691. https://doi.org/10.1001/jamaneurol.2014.367
Papa L, Ramia MM, Kelly JM, Burks SS, Pawlowicz A, Berger RP. Systematic review of clinical research on biomarkers for pediatric traumatic brain injury. J Neurotrauma. 2013;30:324-338. https://doi.org/10.1089/neu.2012.2545
Papa L, Edwards D, Ramia M. Chapter 22 Exploring Serum Biomarkers for Mild Traumatic Brain Injury. Brain Neurotrauma: Molecular, Neuropsychological, and Rehabilitation Aspects. 2015 by Taylor & Francis Group, LLC. Bookshelf ID: NBK299199PMID: 26269900.
Jagoda AS, Bazarian JJ, Bruns JJ Jr, Cantrill SV, Gean AD, Howard PK, et al. Clinical policy: Neuroimaging and decisionmaking in adult mild traumatic brain injury in the acute setting. Ann Emerg Med. 2008;52:714-748. https://doi.org/10.1016/j.jen.2008.12.010
Huang MX, Theilmann RJ, Robb A, Angeles A, Nichols S, Drake A, et al. Integrated imaging approach with MEG and DTI to detect mild traumatic brain injury in military and civilian patients. J Neurotrauma. 2009;26:1213-1226. https://doi.org/10.1089/neu.2008.0672
Galgano M, Toshkezi G, Qiu X, Russell T, Chin L, Zhao LR. Traumatic Brain Injury. Current Treatment Strategies and Future Endeavors. Cell Transplant. 2017;26(7):1118-1130. https://doi.org/10.1177/0963689717714102