Background. A study was organized to determine age-specific Russian reference intervals (RIs) for AMH for women and men and to compare the newly derived RIs with those provided in literature for other populations and by the manufacturer of the assay. The correlation between age, AMH concentration and antral follicle count (AFC) was estimated. Materials and methods. By use of CLSI EP 28AC protocol, 460 healthy 18—60 year old (yo) volunteers including 436 women and 24 men were recruited. AMH was measured on the Access 2 immunochemistry analyzer (Beckman Coulter, Inc.) in CIR laboratory in Moscow. Blood collected on any day, AFC determined on day 1—4 of menstrual cycle in women. Transvaginal ultrasound (TVUS) was performed by sonographers using Medison Ultrasound Systems. AFC was defined as the sum of follicles 2—10 mm in diameter in both ovaries. The women were divided into groups with non-detectable, low (<10) and normal (11—20) number of follicles in TVUS. To explore RIs, non-parametric analysis was used (statistical program Stata 11). Pregnant women, women with polycystic ovary syndrome (PCOS) and undergoing ovarian surgery were excluded from the investigation. Partition by age for women from 18 to 60 yo was performed. Results. In the Russian population in groups of women 26—30 and 31—35 yo median and upper limit (UL) for AMH were significantly higher than those provided by the manufacturer (median 3.38 vs. 2.27; 2.85 vs. 1.88; UL 11.03 vs. 7.37, 11.61 vs. 7.35, respectively). In comparison to a Brazilian investigation the median and UL in Russian population were lower for group 18—30 yo (median: 3.35 vs. 3.7; UL 11.7 vs 12.5, respectively). For groups of women 41—45 yo, >46 yo and men RIs were verified to those provided by the manufacturer according to CLSI protocol. Significant negative correlation between AMH and age in women was shown starting from 35 yo (rp= –0.46); correlation between AMH concentration and AFC in both ovaries was demonstrated (rp=0.64; 0.69); the significant difference between AMH levels in groups of the same age but with different AFC (low vs normal number of follicles) was shown (1.15 vs 2.6 respectively). Conclusion. For the first time RIs for AMH, specific for Russian population using Access AMH assay manufactured by Beckman Coulter Inc. were established. The comparison with available investigations dedicated to RIs was performed. Correlation between AMH concentration, age and AFC was shown.