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Negasheva E.S.

Moscow Scientific and Practical Center of Dermatovenerology and Cosmetology

Vasiltsova M.Yu.

Moscow Scientific and Practical Center of Dermatovenerology and Cosmetology

Frigo N.V.

Moscow Scientific and Practical Center of Dermatovenerology and Cosmetology;
State Research Center — Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency

Gushchin A.E.

Moscow Scientific and Practical Center of Dermatovenerology and Cosmetology

Dolya O.V.

Moscow Scientific and Practical Center of Dermatovenerology and Cosmetology;
Peoples’ Friendship University of Russia

Kitaeva N.V.

Moscow Scientific and Practical Center of Dermatovenerology and Cosmetology

Mardanli S.G.

State University of Humanities and Technology (GGTU)

Zatorskaya N.F.

Moscow Scientific and Practical Center of Dermatovenerology and Cosmetology

Laboratory diagnosis of «key» sexually transmitted infections. Part II

Authors:

Negasheva E.S., Vasiltsova M.Yu., Frigo N.V., Gushchin A.E., Dolya O.V., Kitaeva N.V., Mardanli S.G., Zatorskaya N.F.

More about the authors

Journal: Russian Journal of Clinical Dermatology and Venereology. 2024;23(1): 6‑11

DOI: 10.17116/klinderma2024230116

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To cite this article:

Negasheva ES, Vasiltsova MYu, Frigo NV, et al. . Laboratory diagnosis of «key» sexually transmitted infections. Part II. Russian Journal of Clinical Dermatology and Venereology. 2024;23(1):6‑11. (In Russ.)
https://doi.org/10.17116/klinderma2024230116

Подписка 2025 (Russian Journal of Clinical Dermatology and Venereology)
 
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The review summarizes information on laboratory methods for the diagnosis of sexually transmitted infections (STIs) prevalent in the Russian Federation caused by bacterial and protozoan pathogens, namely T. vaginalis and M. genitalium. The estimation and location of each method in the diagnosis of these infections were given. It was shown that the absolute priority in the diagnosis of infections caused by T. vaginalis and M. genitalium are direct methods of identification, among which preference is currently given to molecular biological methods with high sensitivity and specificity, rapid results (compared to the culture method) without the presence of a living agent that allows the simultaneous identification of several pathogens in one sample of biomaterial and quantification DNA/RNA pathogens (Real-time PCR). The nucleic acid amplification technique (NAAT) for simultaneous identification of 4 «key» STIs pathogens (N. gonorrhoeae, C. trachomatis, M. genitalium, T. vaginalis), which increases the effectiveness of diagnosis of these infections, has been noted to be extremely popular in modern conditions. In addition, the clinical material for the study in NAAT in men is the first portion of urine, the urethral discharge, in women — the discharge from urethra, cervical canal, the first portion of urine.

Keywords:

sexually transmitted infections
diagnosis
M.genitalium
T.vaginalis
N.gonorrhoeae
C.trachomatis
molecular biological methods
NAAT

Authors:

Negasheva E.S.

Moscow Scientific and Practical Center of Dermatovenerology and Cosmetology

Vasiltsova M.Yu.

Moscow Scientific and Practical Center of Dermatovenerology and Cosmetology

Frigo N.V.

Moscow Scientific and Practical Center of Dermatovenerology and Cosmetology;
State Research Center — Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency

Gushchin A.E.

Moscow Scientific and Practical Center of Dermatovenerology and Cosmetology

Dolya O.V.

Moscow Scientific and Practical Center of Dermatovenerology and Cosmetology;
Peoples’ Friendship University of Russia

Kitaeva N.V.

Moscow Scientific and Practical Center of Dermatovenerology and Cosmetology

Mardanli S.G.

State University of Humanities and Technology (GGTU)

Zatorskaya N.F.

Moscow Scientific and Practical Center of Dermatovenerology and Cosmetology

Received:

09.03.2023

Accepted:

16.11.2023

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