Development of an enzyme-linked immunosorbent assay system to confirm the presence of immunoglobulins of class G to Treponema pallidum in serum, plasma and cerebrospinal fluid by immune blotting (Western blot format)

Avdonina A.S.

Ecolab, CJCS

Mardanly S.G.

Ecolab, CJCS;
State University of Humanities and Technology

Development of an enzyme-linked immunosorbent assay system to confirm the presence of immunoglobulins of class G to Treponema pallidum in serum, plasma and cerebrospinal fluid by immune blotting (Western blot format)

Authors:

Avdonina A.S., Mardanly S.G.

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Journal: Russian Journal of Clinical Dermatology and Venereology. 2020;19(4): 465‑469

DOI: 10.17116/klinderma202019041465

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To cite this article:

Avdonina AS, Mardanly SG. Development of an enzyme-linked immunosorbent assay system to confirm the presence of immunoglobulins of class G to Treponema pallidum in serum, plasma and cerebrospinal fluid by immune blotting (Western blot format). Russian Journal of Clinical Dermatology and Venereology. 2020;19(4):465‑469. (In Russ.)
https://doi.org/10.17116/klinderma202019041465

Подписка 2025-2 (Russian Journal of Clinical Dermatology and Venereology)

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RELEVANCE

In laboratory diagnostics of syphilis, the most effective confirmatory diagnostic technique is immune blotting. In the Russian Federation, reagent kits for these diagnostic techniques were registered only in the Line blot format using the recombinant analogues of Treponema pallidum antigens.

OBJECTIVE

To develop a reagent kit for the diagnosis of syphilis by immune blotting in Western blot format using native Treponema pallidum antigens.

MATERIAL AND METHODS

The immunosorbent was prepared using native T. pallidum lysate (Nichols strain) of our own production and commercial preparations necessary for electrophoresis and electrotransfer of pathogen proteins to the nitrocellulose membrane. The optimal mode of conducting these procedures was selected. To assess the sensitivity and specificity of the new test system, commercial panels of serums containing and not containing antibodies to T. pallidum were used, as well as clinical materials - samples of serum, blood plasma, and cerebrospinal fluid.

RESULTS

A new test system has been developed to assess the presence of antibodies of class G in the blood and cerebrospinal fluid against specific T. pallidum antigens by Western blot immune blotting, which is not inferior in effectiveness to the imported counterpart.

Keywords:

diagnostics

syphilis

immune blotting

Authors:

Avdonina A.S.

Ecolab, CJCS

Mardanly S.G.

Ecolab, CJCS;
State University of Humanities and Technology

Received:

24.04.2020

Accepted:

25.05.2020

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