Host genetic polymorphisms as risk factors for H. pylori-positive peptic ulcer

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OBJECTIVE

To study the role of genetic variants of candidate genes (rs2294008 of the PSCA gene, rs6136 of the SELP gene, rs505922, rs8176720, rs2519093, rs507666 of the ABO gene, rs651007, rs579459, and rs649129 of the ABO/RF00019 gene) in the development of H.pylori-positive PUD.

MATERIALS AND METHODS

In the current study, there were 347 individuals in the control group and 98 patients with H.pylori-positive PUD. The assessment of the regulatory potential of the SNPs selected for study was carried out using the Internet resources HaploReg v. 4.1, PolyPhen-2, and GTEx Portal, and genotyping was carried out by the polymerase chain reaction (PCR). The logistic regression method was used for association analysis (allelic, additive, dominant, and recessive genetic models).

RESULTS

The C allele of the SELP gene (rs6136) is a genetic risk factor for the development of H. pylori-positive PUD (OR=1.66; 95% CI=1.02—2.71; p=0.041; p_perm=0.042). The rs6136 polymorphism is non-synonymous and has a strong regulatory potential. The rs6136 allele C, which raises the chance of developing H.pylori-positive PUD, is linked to increased F5 gene expression and decreased BLZF1 gene alternative splicing.

CONCLUSIONS

The C allele of the SELP gene (rs6136) is a genetic risk factor for the development of H.pylori-positive PUD.

Keywords:

Single nucleotide polymorphism

Helicobacter pylori

peptic ulcer

risk factors

genotype

Authors:

Rashina O.V.

Belgorod National Research University

Churnosov M.I.

Belgorod State National Research University

ORCID: 0000-0003-1254-6134

Received:

02.10.2022

Accepted:

18.01.2023

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