The site of the Media Sphera Publishers contains materials intended solely for healthcare professionals.
By closing this message, you confirm that you are a certified medical professional or a student of a medical educational institution.

Login
EN
+7 (495) 482-4118
+7 (495) 482-4329
+8 800 250-18-12
  • (toll-free number for subscriptions)
    Mon-Fri from 10 a.m. to 6 p.m.

  • © 1998-2025
+7 (495) 482-43-29
EN
All journals
Journal
Year
Year
Search result: 0

Kalinin D.V.

National Medical Research Center of Surgery named after A.V. Vishnevsky of Ministry of health of the Russian Federation

Samoylova D.V.

N.N. Blokhin National Medical Research Center of Oncology

Danilova N.V.

Lomonosov Moscow State University

Germanovich N.Yu.

A.V. Vishnevsky National Medical Research Center of Surgery

Kuznetsova O.A.

Russian Medical Academy of Continuous Professional Education

Zavalishina L.E.

Russian Medical Academy of Continuous Professional Education

Comparative immunohistochemistry study of different antibodies clones for detection of breast cancer markers (estrogen receptor, progesterone receptor, HER2/neu, Ki-67)

Authors:

Kalinin D.V., Samoylova D.V., Danilova N.V., Germanovich N.Yu., Kuznetsova O.A., Zavalishina L.E.

More about the authors

Journal: Russian Journal of Archive of Pathology. 2024;86(2): 14‑20

DOI: 10.17116/patol20248602114

Read: 1190 times

Download PDF (RU)
Contact the author
Table of contents

COMPARATIVE IMMUNOHISTOCHEMISTRY STUDY OF DIFFERENT ANTIBODIES CLONES FOR DETECTION OF BREAST CANCER MARKERS (ESTROGEN RECEPTOR, PROGESTERONE RECEPTOR, HER2/NEU, KI-67)
COMPARATIVE IMMUNOHISTOCHEMISTRY STUDY OF DIFFERENT ANTIBODIES CLONES FOR DETECTION OF BREAST CANCER MARKERS (ESTROGEN RECEPTOR, PROGESTERONE RECEPTOR, HER2/NEU, KI-67)

To cite this article:

Kalinin DV, Samoylova DV, Danilova NV, Germanovich NYu, Kuznetsova OA, Zavalishina LE. Comparative immunohistochemistry study of different antibodies clones for detection of breast cancer markers (estrogen receptor, progesterone receptor, HER2/neu, Ki-67). Russian Journal of Archive of Pathology. 2024;86(2):14‑20. (In Russ.)
https://doi.org/10.17116/patol20248602114

Подписка 2026 Архив патологии (Russian Journal of Archive of Pathology)
МСФ на ЯМ
Использование инфографики авторами научных работ
Close metadata
Recommended articles:
NDRG1 protein expression in breast cancer samples, rela­tionship with lymph nodes meta­stasis. Russian Journal of Archive of Pathology. 2024;(6):36-42
Morphofunctional characteristics of the endo­metrium in patients of reproductive age with chro­nic salpingo-oophoritis. Russian Journal of Archive of Pathology. 2024;(6):49-57
Cardiac myxoma: biological features, morphology, differential diagnosis. Russian Journal of Archive of Pathology. 2024;(6):74-81
Capsular contracture after reco­nstructive plastic surgery for breast cancer. P.A. Herzen Journal of Onco­logy. 2024;(6):78-83
Impact of tumor size on actuarial survival of patients with primary breast sarcoma: a case-control study. Piro­gov Russian Journal of Surgery. 2024;(12):38-43
Asse­ssment of risk factors for breast cancer deve­lopment in women living in the Sara­tov region. Russian Bulletin of Obstetrician-Gynecologist. 2024;(6):100-106
Differential Diagnosis of Perianal Skin Neoplasms. Russian Journal of Clinical Dermatology and Vene­reology. 2024;(6):673-678
Clinical Case of HIV-Associated Kaposi’s Sarcoma. Russian Journal of Clinical Dermatology and Vene­reology. 2024;(6):782-786
Left ventricular myocardial performance index (Tei index) as an early marker of cardiotoxicity in anti­tumor therapy. Russian Cardiology Bulletin. 2024;(4-2):137-143
Nottingham Prognostic Index rela­tion to cyto­kine-producing tumor mole­cular subtypes in breast cancer. Russian Journal of Preventive Medi­cine. 2025;(1):63-68
Abstract:

OBJECTIVE

A comparative study of detection of breast cancer markers (estrogen receptors, progesterone receptors, HER2/neu, Ki-67) by immunohistochemical method with antibodies produced by PrimeBioMed (Russia) and antibodies produced by Roche Ventana (USA).

MATERIAL AND METHODS

Surgical specimens and biopsies from 37 patients with invasive breast cancer were used. Sections were stained with antibodies of clones ER SP1 and GM030, PR 1E2 and PBM-5B8, HER2/neu 4B5 and PBM-46A6, Ki-67 30-9 and GM010.

RESULTS

There was a high positive and significant correlation between the immunohistochemistry results and antibodies of the clones ER-SP1 and GM030, PR1E2 and PBM-5B8, HER2/neu4B5 and PBM-46A6, Ki-67 30-9 and GM010.

CONCLUSION

The study showed the possibility of using antibodies of clones GM030, HER2/neu 4B5, PBM-46A6, GM010 (PrimeBioMed) on the Ventana Bench Marck Ultra automatic immunostainer using the detection system UltraView Universal DAB Detection Kit.

Keywords:

breast cancer
estrogen receptors
progesterone receptors
HER2/neu
Ki-67
immunohistochemistry

Authors:

Kalinin D.V.

National Medical Research Center of Surgery named after A.V. Vishnevsky of Ministry of health of the Russian Federation

Samoylova D.V.

N.N. Blokhin National Medical Research Center of Oncology

Danilova N.V.

Lomonosov Moscow State University

Germanovich N.Yu.

A.V. Vishnevsky National Medical Research Center of Surgery

Kuznetsova O.A.

Russian Medical Academy of Continuous Professional Education

Zavalishina L.E.

Russian Medical Academy of Continuous Professional Education

Received:

29.02.2024

Accepted:

04.03.2024

Close metadata

References:

  1. Ferlay J, Colombet M, Soerjomataram I, Mathers C, Parkin DM, Piñeros M, Znaor A, Bray F. Estimating the global cancer incidence and mortality in 2018: GLOBOCAN sources and methods. Int J Cancer. 2019;144(8):1941-1953. https://doi.org/10.1002/ijc.31937
  2. Kaprin AD, Starinskii VV, Shakhzadova AO, eds. Zlokachestvennye novoobrazovaniya v Rossii v 2020 godu (zabolevaemost’ i smertnost’). Moscow: MNIOI im. P.A. Gertsena — filial FGBU «NMITs radiologii Ministerstva zdravookhraneniya Rossiiskoi Federatsii; 2021. (In Russ.).
  3. Giaquinto AN, Sung H, Miller KD, Kramer JL, Newman LA, Minihan A, Jemal A, Siegel RL. Breast cancer statistics, 2022. CA Cancer J Clin. 2022;72(6):524-541.  https://doi.org/10.3322/caac.21754
  4. Ministerstvo zdravookhraneniya Rossiiskoi Federatsii. Klinicheskie rekomendatsii. Rak molochnoi zhelezy. 2021. Accessed 14.08.023. (In Russ.). https://cr.minzdrav.gov.ru/recomend/379_4
  5. Frank GA, Zavalishina LE, Andreeva YuYu, eds. Breast cancer. Morphological diagnostics and genetics. A guide for doctors. 2nd ed. Moscow: Prakticheskaya meditsina; 2021. (In Russ.).
  6. Kurozumi S, Matsumoto H, Hayashi Y, Tozuka K, Inoue K, Horiguchi J, Takeyoshi I, Oyama T, Kurosumi M. Power of PgR expression as a prognostic factor for ER-positive/HER2-negative breast cancer patients at intermediate risk classified by the Ki67 labeling index. BMC Cancer. 2017;17(1):354.  https://doi.org/10.1186/s12885-017-3331-4
  7. Zavalishina LE, Olyushina EM, Andreeva YuYu, Kuznetsova OA, Moskvina LV, Frank GA. Updated CAP guidelines for determining molecular biological subtypes of breast cancer. Arkhiv Patologii. 2023;85(4):39-46. (In Russ.). https://doi.org/10.17116/patol20238504139
  8. Wolff AC, Somerfield MR, Dowsett M, Hammond MEH, Hayes DF, McShane LM, Saphner TJ, Spears PA, Allison KH. Human epidermal growth factor receptor 2 testing in breast cancer. Arch Pathol Lab Med. 2023;147(9):993-1000. https://doi.org/10.5858/arpa.2023-0950-SA
  9. Schnitt SJ, Tarantino P, Collins LC. The American Society of Clinical Oncology-College of American Pathologists Guideline Update for human epidermal growth factor receptor 2 testing in breast cancer. Arch Pathol Lab Med. 2023;147(9):991-992.  https://doi.org/10.5858/arpa.2023-0187-ED
  10. Allred DC, Harvey JM, Berardo M, Clark GM. Prognostic and predictive factors in breast cancer by immunohistochemical analysis. Mod Pathol. 1998;11(2):155-168. 
  11. The WHO Classification of Tumours Editorial Board. WHO Classification of Tumours. Breast tumours. 5th ed. Vol. 2. Lyon: IARC-Press; 2019.
  12. Wolff AC, Hammond ME, Hicks DG, Dowsett M, McShane LM, Allison KH, Allred DC, Bartlett JM, Bilous M, Fitzgibbons P, et al.; American Society of Clinical Oncology; College of American Pathologists. Recommendations for human epidermal growth factor receptor 2 testing in breast cancer: American Society of Clinical Oncology/College of American Pathologists clinical practice guideline update. Arch Pathol Lab Med. 2014;138(2):241-256.  https://doi.org/10.5858/arpa.2013-0953-SA
  13. Lott R, Tunnicliffe J, Sheppard E, Santiago J, Hladik C, Nasim M, Zeitner K, Haas T, Kohl S, Movahedi-Lankareni S. Practical guide to specimen handling in surgical pathology. College of American Pathologists. Version: 10.0. 2022.
  14. Rüschoff J, Friedrich M, Nagelmeier I, Kirchner M, Andresen LM, Salomon K, Portier B, Sredni ST, Schildhaus HU, Jasani B, et al. Comparison of HercepTest mAb pharmDx (Dako Omnis, GE001) with Ventana PATHWAY anti-HER-2/neu (4B5) in breast cancer: correlation with HER2 amplification and HER2 low status. Virchows Arch. 2022;481(5):685-694.  https://doi.org/10.1007/s00428-022-03378-5
Contact the author
Email
Message
The publishing house "Media Sphere" does not provide treatment services, does not give recommendations on contacting medical institutions and specific specialists, on the prescription of medicines and dietary supplements.

Email Confirmation

An email was sent to test@gmail.com with a confirmation link. Follow the link from the letter to complete the registration on the site.

Email Confirmation

Contact us
If you have any questions, complaints or suggestions, please use this feedback form.
Email
Message
The publishing house "Media Sphere" does not provide treatment services, does not give recommendations on contacting medical institutions and specific specialists, on the prescription of medicines and dietary supplements.
Submit Application

You can become an author of one of our magazines, send a request and we will consider it in during the day.

Name
Phone
E-mail
Message
Login
Registration
E-mail
Password
Forgot Password?

Log in to the site using your account in one of the services

Password recovery
E-mail
Login
Register

We use cооkies to improve the performance of the site. By staying on our site, you agree to the terms of use of cооkies. To view our Privacy and Cookie Policy, please. click here.