The site of the Media Sphera Publishers contains materials intended solely for healthcare professionals.
By closing this message, you confirm that you are a certified medical professional or a student of a medical educational institution.

Login
EN
+7 (495) 482-4118
+7 (495) 482-4329
+8 800 250-18-12
  • (toll-free number for subscriptions)
    Mon-Fri from 10 a.m. to 6 p.m.

  • © 1998-2026
+7 (495) 482-43-29
EN
All journals
Journal
Year
Year
Search result: 0

Demura T.A.

Sechenov First Moscow State Medical University

Fisenko N.V.

M.M. Krasnov Research Institute of Eye Diseases

Osipyan G.A.

M.M. Krasnov Research Institute of Eye Diseases

Afonina M.A.

I.M. Sechenov First Moscow State Medical University

Corneal changes in Fuchs endothelial corneal dystrophy and bullous keratopathy

Authors:

Demura T.A., Fisenko N.V., Osipyan G.A., Afonina M.A.

More about the authors

Journal: Russian Journal of Archive of Pathology. 2023;85(5): 29‑35

DOI: 10.17116/patol20238505129

Read: 2172 times

Download PDF (RU)
Contact the author
Table of contents

CORNEAL CHANGES IN FUCHS ENDOTHELIAL CORNEAL DYSTROPHY AND BULLOUS KERATOPATHY
CORNEAL CHANGES IN FUCHS ENDOTHELIAL CORNEAL DYSTROPHY AND BULLOUS KERATOPATHY

To cite this article:

Demura TA, Fisenko NV, Osipyan GA, Afonina MA. Corneal changes in Fuchs endothelial corneal dystrophy and bullous keratopathy. Russian Journal of Archive of Pathology. 2023;85(5):29‑35. (In Russ.)
https://doi.org/10.17116/patol20238505129

Подписка 2026 Архив патологии (Russian Journal of Archive of Pathology)
МСФ на ЯМ
Использование инфографики авторами научных работ
Close metadata
Recommended articles:
Morphometric analysis of small reti­noblastomas following combination chemotherapy. Russian Annals of Ophthalmology. 2025;(2):38-43
Intravascular physiology and imaging in dete­rmining the indi­cations for reva­scularization in patients with inte­rmediate coro­nary stenosis. Russian Journal of Cardiology and Cardiovascular Surgery. 2025;(3):255-260
Diagnostic capa­bilities of opti­cal cohe­rence tomo­graphy in obstruction of the upper lacrimal pathway. Russian Annals of Ophthalmology. 2025;(3):37-47
OCT patterns as a source of errors in the diagnosis of intraocular tumors. Russian Annals of Ophthalmology. 2025;(3):63-70
Nano­second reti­nal laser therapy: three years of practical expe­rience. Russian Annals of Ophthalmology. 2025;(4):60-65
Mole­cular diagnostic biomarkers of subretinal fluid in asse­ssing the seve­rity and prognosis of rhegmatogenous reti­nal deta­chment. Russian Annals of Ophthalmology. 2025;(6):119-126
Clinical and economic impact of broader implementation of opti­cal cohe­rence tomo­graphy and anti-VEGF therapy for diabetic reti­nopathy and macu­lar edema in type 1 diabetes. Medi­cal Technologies. Asse­ssment and Choice. 2026;(1):122-133
Expe­rimental study of a new anti-glaucoma intrascleral implant. Russian Annals of Ophthalmology. 2026;(2):16-20
Clinical case of an inhe­rited reti­nal disease with late mani­festation. Russian Annals of Ophthalmology. 2026;(2):50-55
Antiangiogenic therapy after cata­ract surgery in neovascular age-related macu­lar dege­neration (clinical obse­rvation). Russian Annals of Ophthalmology. 2026;(2):57-62
Abstract:

OBJECTIVE

Evaluation of structural and immunohistochemical features of cornea in Fuchs endothelial corneal dystrophy (FECD) and bullous keratopathy (BK).

MATERIAL AND METHODS

Group 1 — 44 patients (46 eyes) with FECD, group 2 — 42 patients (42 eyes) with BK. All patients underwent keratoplasty. Preoperative anterior segment optical coherence tomography (AS-OCT, RTVue-100, Optovue, USA) was performed. Endothelium—Descemet membrane (EDM) complexes, corneal buttons were obtained intraoperatively. Morphological (H&E staining) and immunohistochemical (primary antibodies to pancytokeratin, vimentin, fibronectin) studies were performed at the light microscope level (Leica DM-2500, Leica Application Suite V4.8, Leica Microsystems, Switzerland).

RESULTS

A direct correlation is found between the results of DM analysis in vivo with OCT and ex vivo with light microscopy. DM thickness (AS-OCT) was significantly greater in FECD (23.0 [19.0; 27.0] μm), than in BK (13.0 [12.0; 14.0] μm). Morphological study of EDM and corneal buttons showed similar difference in DM thickness: 17.9 [16.1; 20.0] μm in FECD and 11.9 [11.3; 13.0] μm in BK. Irregular optical density of stroma is a feature of edema and local fibrosis. In FECD and BK pancytokeratin is expressed in epithelial and endothelial cells, vimentin — in keratocytes, macrophages and vascular endothelium, fibronectin — in DM. In FECD, vimentin is expressed in endothelial cells.

CONCLUSION

FECD and BK are associated with different DM’ and endothelium’ abnormalities, which lead to similar changes of stroma and epithelium. AS-OCT is a useful method of FECD and BK in vivo diagnostics and the selection of treatment option.

Keywords:

Fuchs endothelial corneal dystrophy
bullous keratopathy
optical coherence tomography

Authors:

Demura T.A.

Sechenov First Moscow State Medical University

Fisenko N.V.

M.M. Krasnov Research Institute of Eye Diseases

Osipyan G.A.

M.M. Krasnov Research Institute of Eye Diseases

Afonina M.A.

I.M. Sechenov First Moscow State Medical University

Received:

14.04.2023

Accepted:

21.06.2023

Close metadata

References:

  1. Feizi S. Corneal endothelial cell dysfunction: etiologies and management. Ther Adv Ophthalmol. 2018;10:2515841418815802. https://doi.org/10.1177/2515841418815802
  2. Price MO, Mehta JS, Jurkunas UV, Price FW Jr. Corneal endothelial dysfunction: evolving understanding and treatment options. Prog Retin Eye Res. 2021;82:100904. https://doi.org/10.1016/j.preteyeres.2020.100904
  3. Ang M, Baskaran M, Werkmeister RM, Chua J, Schmidl D, Aranha Dos Santos V, Garhöfer G, Mehta JS, Schmetterer L. Anterior segment optical coherence tomography. Prog Retin Eye Res. 2018;66:132-156.  https://doi.org/10.1016/j.preteyeres.2018.04.002
  4. Gupta N, Varshney A, Ramappa M, Basu S, Romano V, Acharya M, Gaur A, Kapur N, Singh A, Shah G, et al. Role of AS-OCT in managing corneal disorders. Diagnostics (Basel). 2022;12(4):918.  https://doi.org/10.3390/diagnostics12040918
  5. Azarcon CP, Wells JR, Grossniklaus HE. Multimodal imaging and histopathology features of a corneal keloid. Ophthalmology. 2022;129(4):378.  https://doi.org/10.1016/j.ophtha.2021.09.017
  6. Shousha MA, Perez VL, Wang J, Ide T, Jiao S, Chen Q, Chang V, Buchser N, Dubovy SR, Feuer W, et al. Use of ultra-high-resolution optical coherence tomography to detect in vivo characteristics of Descemet’s membrane in Fuchs’ dystrophy. Ophthalmology. 2010;117(6):1220-1227. https://doi.org/10.1016/j.ophtha.2009.10.027
  7. Vajzovic LM, Karp CL, Haft P, Shousha MA, Dubovy SR, Hurmeric V, Yoo SH, Wang J. Ultra high-resolution anterior segment optical coherence tomography in the evaluation of anterior corneal dystrophies and degenerations. Ophthalmology. 2011;118(7):1291-1296. https://doi.org/10.1016/j.ophtha.2010.12.015
  8. Brockmann T, Brockmann C, Maier AB, Schroeter J, Bertelmann E, Torun N. Primary Descemet’s membrane endothelial keratoplasty for Fuchs endothelial dystrophy versus bullous keratopathy: histopathology and clinical results. Curr Eye Res. 2018;43(10):1221-1227. https://doi.org/10.1080/02713683.2018.1490773
  9. Sridhar MS. Anatomy of cornea and ocular surface. Indian J Ophthalmol. 2018;66(2):190-194.  https://doi.org/10.4103/ijo.IJO_646_17
  10. Kasparova EuA, Subbot AM, Kalinina DB. Proliferative potential of human corneal endothelium. Vestnik Oftal’mologii. 2013; 129(3):82-88. (In Russ.).
  11. Hidayat AA, Cockerham GC. Epithelial metaplasia of the corneal endothelium in Fuchs endothelial dystrophy. Cornea. 2006;25(8): 956-959.  https://doi.org/10.1097/01.ico.0000228786.84581.ee
  12. Okumura N, Minamiyama R, Ho LT, Kay EP, Kawasaki S, Tourtas T, Schlötzer-Schrehardt U, Kruse FE, Young RD, Quantock AJ, et al. Involvement of ZEB1 and Snail1 in excessive production of extracellular matrix in Fuchs endothelial corneal dystrophy. Lab Invest. 2015;95(11):1291-1304. https://doi.org/10.1038/labinvest.2015.111
  13. Weller JM, Zenkel M, Schlötzer-Schrehardt U, Bachmann BO, Tourtas T, Kruse FE. Extracellular matrix alterations in late-onset Fuchs’ corneal dystrophy. Invest Ophthalmol Vis Sci. 2014;55(6):3700-3708. https://doi.org/10.1167/iovs.14-14154
  14. Kaluzny BJ, Szkulmowska A, Szkulmowski M, Bajraszewski T, Kowalczyk A, Wojtkowski M. Fuchs’ endothelial dystrophy in 830-nm spectral domain optical coherence tomography. Ophthalmic Surg Lasers Imaging. 2009;40(2):198-200.  https://doi.org/10.3928/15428877-20090301-11
  15. De Roo AK, Janssens T, Foets B, van den Oord JJ. Immunohistochemical profiling of corneas with Fuchs endothelial corneal dystrophy. Cornea. 2017;36(7):866-874.  https://doi.org/10.1097/ICO.0000000000001212
  16. Liu T, Xu Y, Sun D, Xie L. Histological evaluation of corneal scar formation in pseudophakic bullous keratopathy. PLoS One. 2012;7(6):e39201. https://doi.org/10.1371/journal.pone.0039201
  17. Ljubimov AV, Burgeson RE, Butkowski RJ, Couchman JR, Wu RR, Ninomiya Y, Sado Y, Maguen E, Nesburn AB, Kenney MC. Extracellular matrix alterations in human corneas with bullous keratopathy. Invest Ophthalmol Vis Sci. 1996;37(6):997-1007.
  18. Morishige N, Sonoda KH. Bullous keratopathy as a progressive disease: evidence from clinical and laboratory imaging studies. Cornea. 2013;32(suppl 1):77-83.  https://doi.org/10.1097/ICO.0b013e3182a1bc65
  19. Wertheimer CM, Elhardt C, Wartak A, Luft N, Kassumeh S, Dirisamer M, Siedlecki J, Vounotrypidis E, Priglinger SG, Mayer WJ. Corneal optical density in Fuchs endothelial dystrophy determined by anterior segment optical coherence tomography. Eur J Ophthalmol. 2021;31(4):1771-1778. https://doi.org/10.1177/1120672120944796
  20. Castro-Muñozledo F, Meza-Aguilar DG, Domínguez-Castillo R, Hernández-Zequinely V, Sánchez-Guzmán E. Vimentin as a marker of early differentiating, highly motile corneal epithelial cells. J Cell Physiol. 2017;232(4):818-830.  https://doi.org/10.1002/jcp.25487
  21. Thaung C, Davidson AE. Fuchs endothelial corneal dystrophy: current perspectives on diagnostic pathology and genetics-Bowman Club Lecture. BMJ Open Ophthalmol. 2022;7(1):e001103. https://doi.org/10.1136/bmjophth-2022-001103
Contact the author
Email
Message
The publishing house "Media Sphere" does not provide treatment services, does not give recommendations on contacting medical institutions and specific specialists, on the prescription of medicines and dietary supplements.

Email Confirmation

An email was sent to test@gmail.com with a confirmation link. Follow the link from the letter to complete the registration on the site.

Email Confirmation

Submit Application

You can become an author of one of our magazines, send a request and we will consider it in during the day.

Name
Phone
E-mail
Message
Login
Registration
E-mail
Password
Forgot Password?

Log in to the site using your account in one of the services

Password recovery
E-mail
Login
Register

We use cооkies to improve the performance of the site. By staying on our site, you agree to the terms of use of cооkies. To view our Privacy and Cookie Policy, please. click here.