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Yusef Yu.

Krasnov Research Institute of Eye Diseases;
I.M. Sechenov First Moscow State Medical University (Sechenov University)

Petrachkov D.V.

Krasnov Research Institute of Eye Diseases

Baryshev K.V.

Krasnov Research Institute of Eye Diseases

Matyuschenko A.G.

Krasnov Research Institute Of Eye Diseases

The effect of vitrectomy on changes in molecular biomarker levels of the vitreous cavity in the early postoperative period in proliferative diabetic retinopathy

Authors:

Yusef Yu., Petrachkov D.V., Baryshev K.V., Matyuschenko A.G.

More about the authors

Journal: Russian Annals of Ophthalmology. 2025;141(2): 30‑36

DOI: 10.17116/oftalma202514102130

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Table of contents

THE EFFECT OF VITRECTOMY ON CHANGES IN MOLECULAR BIOMARKER LEVELS OF THE VITREOUS CAVITY IN THE EARLY POSTOPERATIVE PERIOD IN PROLIFERATIVE DIABETIC RETINOPATHY
THE EFFECT OF VITRECTOMY ON CHANGES IN MOLECULAR BIOMARKER LEVELS OF THE VITREOUS CAVITY IN THE EARLY POSTOPERATIVE PERIOD IN PROLIFERATIVE DIABETIC RETINOPATHY

To cite this article:

Yusef Yu, Petrachkov DV, Baryshev KV, Matyuschenko AG. The effect of vitrectomy on changes in molecular biomarker levels of the vitreous cavity in the early postoperative period in proliferative diabetic retinopathy. Russian Annals of Ophthalmology. 2025;141(2):30‑36. (In Russ.)
https://doi.org/10.17116/oftalma202514102130

Подписка 2026 Вестник офтальмологии (Russian Annals of Ophthalmology)
 
МСФ на ЯМ
Использование инфографики авторами научных работ
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Abstract:

Determining the levels of molecular biomarkers detected in the vitreous body in various pathological conditions (including diabetic retinopathy), allows for a better understanding of their pathogenesis and optimization of treatment approaches. The multiplex assay is a modern alternative to enzyme-linked immunosorbent assay (ELISA), enabling the simultaneous analysis of a large number of biomarkers.

PURPOSE

This study was performed to identify changes in the levels of vitreous biomarkers in patients with proliferative diabetic retinopathy (PDR) in the early postoperative period following 25-gauge vitrectomy.

MATERIAL AND METHODS

The study included 24 patients (24 eyes) with type 2 diabetes mellitus complicated by PDR requiring surgical intervention for tractional retinal detachment, subtotal or total vitreous hemorrhage, or tractional diabetic macular edema (DME). Vitreous samples were collected from all patients at the first stage of vitrectomy and one month after surgery.

RESULTS

One month after surgery, the level of the pro-inflammatory cytokine vascular endothelial growth factor (VEGF) decreased from 203.8±228.0 to 113±95 pg/ml; however, the difference was not statistically significant (p=0.307). A significant reduction was observed in the concentrations of the following pro-inflammatory biomarkers: bFGF, CTACK, Eotaxin, HGF, IL-1β, -1α, -4, -12 (p40), -16, -18, IL-2Rα, IP-10, MIG, SCF, SCGF-β, SDF-1α, MIF, and M-CSF.

CONCLUSION

The main effect of vitrectomy on pro-inflammatory cytokines is reduction of their concentration, which confirms the buffering role of the vitreous body in the accumulation of molecular biomarkers in diabetic retinopathy.

Keywords:

diabetic retinopathy
molecular biomarkers
vitrectomy
multiplex assay

Authors:

Yusef Yu.

Krasnov Research Institute of Eye Diseases;
I.M. Sechenov First Moscow State Medical University (Sechenov University)

Petrachkov D.V.

Krasnov Research Institute of Eye Diseases

Baryshev K.V.

Krasnov Research Institute of Eye Diseases

Matyuschenko A.G.

Krasnov Research Institute Of Eye Diseases

Received:

10.11.2024

Accepted:

12.12.2024

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