Results of clinical and immunomorphological analysis in chronic post-traumatic uveitis

Filatova¹, I.A.; Balatskaya N.V.; Kulikova I.G.; Shemetov¹, S.A.; Denisyuk V.O.

doi:https://doi.org/10.17116/oftalma202414006139

Filatova¹, I.A.

Helmholtz National Medical Research Center of Eye Diseases

ORCID: 0000-0002-5930-117X

Balatskaya N.V.

Helmholtz National Medical Research Center of Eye Diseases

ORCID: 0000-0001-8007-6643

Kulikova I.G.

Helmholtz National Medical Research Center of Eye Diseases

ORCID: 0000-0002-1005-5633

Shemetov¹, S.A.

Helmholtz National Medical Research Center of Eye Diseases

ORCID: 0000-0002-4608-5754

Denisyuk V.O.

AO Meditsina (Academician Roytberg’s Clinic)

ORCID: 0000-0003-1769-2843

Results of clinical and immunomorphological analysis in chronic post-traumatic uveitis

Authors:

Filatova¹, I.A., Balatskaya N.V., Kulikova I.G., Shemetov¹, S.A., Denisyuk V.O.

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Journal: Russian Annals of Ophthalmology. 2024;140(6): 39‑44

DOI: 10.17116/oftalma202414006139

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To cite this article:

Filatova¹, IA, Balatskaya NV, Kulikova IG, Shemetov¹, SA, Denisyuk VO. Results of clinical and immunomorphological analysis in chronic post-traumatic uveitis. Russian Annals of Ophthalmology. 2024;140(6):39‑44. (In Russ.)
https://doi.org/10.17116/oftalma202414006139

Подписка 2026 Вестник офтальмологии (Russian Annals of Ophthalmology)

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PURPOSE

This study was conducted to identify the group at highest risk for autoimmune inflammation through a comparative analysis among patients with chronic post-traumatic uveitis (CPTU).

MATERIAL AND METHODS

The clinical group included 50 patients (aged 18 to 87 years, mean age 41±2.6 years) with CPTU resulting from penetrating injury, contusion, or intraocular surgery. All patients underwent comprehensive clinical and diagnostic evaluation, including immunological assessments: leukocyte migration inhibition test (LMIT), analysis of lymphocyte subpopulation composition, and cytokine profile analysis in both blood serum (BS) and aqueous humor (AH).

All patients underwent removal of the blind, non-functional eye with formation of a support-motor stump, followed by morphological examination.

Patients were divided into four groups based on the presence of immune inflammation, confirmed by morphological analysis, as well as organ-specific sensitization to ocular tissue antigens. Group 1 — histology+, LMIT+ (n=36); group 2 — histology+, LMIT– (n=50); group 3 — histology–, LMIT+ (n=12); group 4 — histology–, LMIT– (n=52). The control group consisted of 30 individuals without ocular pathology, matched by sex and age to the main patient groups.

RESULTS

In long-term CPTU, statistically significant alterations in major regulatory subpopulations were observed in group 1, with immune inflammation and a positive LMIT. The findings were confirmed in comparisons with both the control group and across study groups.

CONCLUSION

The clinical and immunomorphological analysis identified an association between decreased levels of transforming growth factor β1 (TGF-β1) in AH and its increased levels in BS, with the risk of autoimmune inflammation in CPTU. The observed changes in the local and systemic cytokine status — reduction of TGF-β1 in AH and increase in BS — may serve as early predictors of autoimmune inflammation, which is important in clinical practice for preventing sympathetic ophthalmia.

Keywords:

chronic post-traumatic uveitis

clinical-immunomorphological analysis

cytokines

autoimmune inflammation prediction

Authors:

Filatova¹, I.A.

Helmholtz National Medical Research Center of Eye Diseases

ORCID: 0000-0002-5930-117X

Balatskaya N.V.

Helmholtz National Medical Research Center of Eye Diseases

ORCID: 0000-0001-8007-6643

Kulikova I.G.

Helmholtz National Medical Research Center of Eye Diseases

ORCID: 0000-0002-1005-5633

Shemetov¹, S.A.

Helmholtz National Medical Research Center of Eye Diseases

ORCID: 0000-0002-4608-5754

Denisyuk V.O.

AO Meditsina (Academician Roytberg’s Clinic)

ORCID: 0000-0003-1769-2843

Received:

05.12.2023

Accepted:

14.01.2024

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References:

Arkhipova LT, Gundorova RA, Kuznetsova IA. Pathogenetic treatment of posttraumatic uveitis. Manual for doctors. Moscow: NIIGB named of Helmholtz; 2002 (In Russ.).
Arkhipova LT. Sympathetic ophthalmia (pathogenesis, pathomorphology, clinic, diagnosis, treatment, prevention). Moscow: Triada; 2006 (In Russ.).
Arkhipova LT, Gundorova RA, Khvatova AV. Epidemiological features and risk factors of sympathetic ophthalmia. Russian Annals of Ophthalmology=Vestnik oftal’mologii. 1996;(4):12-14 (In Russ.).
Khatri A, Timalsena S, Khatri BK, et al. A rare entity: Sympathetic ophthalmia presumably after blunt trauma to the phthisical eye and optical coherence tomography angiography metrics to monitor response to treatment. Clin Case Rep. 2020;8(1):149-154. https://doi.org/10.1002/ccr3.2597
Bechrakis NE, Müller-Stolzenburg NW, Helbig H, et al. Sympathetic ophthalmia following laser cyclocoagulation. Arch Ophthalmol. 1994;112(1):80-84. https://doi.org/10.1001/archopht.1994.01090130090024
Buller AJ, Doris JP, Bonshek R, et al. Sympathetic ophthalmia following severe fungal keratitis. Eye. 2006; 20(11):1306-1307. https://doi.org/10.1038/sj.eye.6702156
Zurochka AV, Maracheva NM, Zurochka VA, et al. Assessment of the level of autoreactive subpopulations of B lymphocytes and regulatory T cells in patients with acute post-traumatic uveitis with penetrating eyeball injury in the dynamics of the inflammatory process. Russian Immunological Journal. 2012;(3):296-303 (In Russ.). https://doi.org/10.21516/2072-0076-2021-14-1-15-20
Maracheva YaM. Immunopathological variants of the course of posttraumatic uveitis with penetrating eyeball injury. Bulletin of the Ural Medical Academic Science. 2012;39(2):42-44 (In Russ.).
Kulikova IG, Slepova OS, Mironkova EA, et al. The role of (sub)population imbalance of blood lymphocytes in the development of systemic organ-specific sensitization in inflammatory and post-traumatic eye pathology. Russian Ophthalmological Journal. 2013;6(4):71-75 (In Russ.).
Filatova IA, Beraya MZ, Harb AH. An improved method of removal of the eyeball with cleavage of the sclera. Ophthalmic Surgery. 2007;(2):28-32 (In Russ.).
Jordan DR, Stoica B. Evisceration with implant placement posterior to posterior sclera. Ophthalm Plast Reconstr Surg. 2016;32(3):178-182. https://doi.org/10.1097/IOP.0000000000000452
Filatova IA, Mohammad IM. Modern approach to eye removal in ophthalmia. Head and Neck. 2017;2(Suppl):52-53 (In Russ.). https://doi.org/10.18821/1993-1859-2017-12-4-210-215
Filatova IA, Mohammad IM, Shemetov S.A. Modification of the eyeball evisceration operation using the technique of radio wave surgery. Russian Ophthalmological Journal. 2017;10(3):84-92 (In Russ.) https://doi.org/10.21516/2072-0076-2017-10-3-84-92
Filatova IA, Mohammad IM. RF Patent 2611932 “Method of eyeball evisceration”. Bul. No. 7. 01.03.2017 (In Russ.).
Filatova IA, Balatskaya NV, Kulikova IG, Denisyuk VO. RF Patent No. 2754292 «A method for predicting a high risk of autoimmune inflammation in post-traumatic uveitis in the long term». Byul. No. 25. 31.08.2021 (In Russ.).