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Kasparova E.A.

Krasnov Research Institute of Eye Diseases

Marchenko N.R.

Research Institute of Eye Diseases

Surnina Z.V.

Krasnov Research Institute of Eye Diseases

Mitichkina T.S.

Research Institute of Eye Diseases

Confocal microscopy in the diagnosis of acanthamoeba keratitis

Authors:

Kasparova E.A., Marchenko N.R., Surnina Z.V., Mitichkina T.S.

More about the authors

Journal: Russian Annals of Ophthalmology. 2021;137(5‑2): 201‑208

DOI: 10.17116/oftalma2021137052201

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Table of contents

CONFOCAL MICROSCOPY IN THE DIAGNOSIS OF ACANTHAMOEBA KERATITIS
CONFOCAL MICROSCOPY IN THE DIAGNOSIS OF ACANTHAMOEBA KERATITIS

To cite this article:

Kasparova EA, Marchenko NR, Surnina ZV, Mitichkina TS. Confocal microscopy in the diagnosis of acanthamoeba keratitis. Russian Annals of Ophthalmology. 2021;137(5‑2):201‑208. (In Russ.)
https://doi.org/10.17116/oftalma2021137052201

Подписка 2026 Вестник офтальмологии (Russian Annals of Ophthalmology)
 
МСФ на ЯМ
Использование инфографики авторами научных работ
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Abstract:

Acanthamoeba keratitis (AK) is a dangerous disease of the cornea, its prevalence has increased significantly due to widespread usage of contact lenses. The similarity of the clinical manifestations of AK to other infectious keratitis (especially herpetic keratitis) requires introduction of a rapid diagnosis method into clinical practice.

PURPOSE

To evaluate the capabilities of corneal confocal microscopy (CCM) in the diagnosis of acanthamoeba keratitis.

MATERIAL AND METHODS

We examined 33 patients (35 eyes) with suspected acanthamoeba keratitis using light and laser confocal microscopy of the cornea (ConfoScan and HRT devices, respectively).

RESULTS

CCM was technically feasible in 23 of 35 cases (65.7%). Acanthamoeba cysts were detected in 16 cases (69.6%); trophozoites were visualized in 17.4% of cases; signs of keratoneuritis were detected in 12 eyes (52.2%); in 7 cases (30.4%), hyporeflective honeycomb-like cavities were observed in the stroma; deep stromal striae were observed in 9 cases (39.1%); activated keratocytes were detected in 11 patients (47.8%), and Langerhans cells were detected in 19 cases (82.6%).

CONCLUSIONS

The main diagnostic value of confocal microscopy is the detection of direct markers of AK — cysts and trophozoites of Acanthamoeba. Both light and laser CCM techniques are suitable for the diagnosis of AK, but the resolution of laser confocal microscopy is higher. The non-invasive nature of this method and the possibility of multiple subsequent examinations make it possible to verify the diagnosis of acanthamoeba keratitis and monitor the treatment.

Keywords:

corneal confocal microscopy
HRT
ConfoScan
acanthamoeba keratitis
cysts
trophozoites
keratoneuritis
activated keratocytes
Langerhans cells

Authors:

Kasparova E.A.

Krasnov Research Institute of Eye Diseases

Marchenko N.R.

Research Institute of Eye Diseases

Surnina Z.V.

Krasnov Research Institute of Eye Diseases

Mitichkina T.S.

Research Institute of Eye Diseases

Received:

30.12.2020

Accepted:

09.02.2021

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