Alteration follicular fluid aminoacid profile after COVID-19

doi:https://doi.org/10.17116/repro20232905125

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INTRODUCTION

Follicular fluid is important component of follicle growth and development. Level of impact of COVID-19 on follicle function is remains understudied.

OBJECTIVE

The aim of the current study is analysis of alteration of follicular fluid aminoacid profile and evalution of assisted reproductive technology program result after COVID-19 for potential prognostic biomarker detection.

MATERIAL AND METHODS

Follicular fluid samples from 237 women were included in the study. Aminoacid profile was analyzed by mass-spectrometry. Manna—Whitney test with significant threshold were used for determination aminoacids with statistical significant level alteration by various COVID-19 anamnesis. For model for determination of outcome result of IVF, which takes into account the severity of COVID-19 logistic regression is used. As independent variable values of level of aminoacids, values of product of levels of aminoacids, values of square of levels of aminoacids. Aminoacids-markers were added in the model by bruteforce with selection according maximal value of area under receive operative curve, which was constructed by 100-times cross-validation with random participation 9/1. Pathway enrichment of aminoacids, dependent on COVID-19 anamnesis, and which included in models, analysis was provided by MetabolAnalyst.

RESULTS

Pathway enrichment of aminoacids, which levels depend on COVID-19 anamnesis and which are included in diagnostic models, analysis demonstrated connection between arginine and arginine’s metabolites levels and success of ART program, probably, by angiogenesis process regulation and homeostasis. Study results indicates possible disruption of regulatory balance of inflammatory response after implantation and early invasion in case of patients after COVID-19, which may be key pathogenetic mechanism in the implementation of reproductive failures.

CONCLUSION

Severe COVID-19 in anamnesis before pregnancy initiation can involve on its outcome. Follicular fluid aminoacids profiling of patients, which take part in ART program after COVID-19 can be used in personalized approach to the choice of the preconception preparation. Study results make possible to assume, that pregnancy initiation and its outcome after COVID-19 can be connected with follicular fluid aminoacids metabolism alteration. It can be used in case of compiling clinical guidelines for women, which are included in the ART program.

Keywords:

SARS-cov-2

COVID-19

IVF

follicular fluid

amino acid profile

metabolomics

mass spectrometry

biomarkers

Received:

18.11.2022

Accepted:

25.12.2022

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References:

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