AlphaB-crystallin expression in eutopic and ectopic endometrium among the patients with peritoneal form endometriosis: pregnancy outcome

Burlev V.A.; Il'iasova N.A.

doi:https://doi.org/10.17116/repro201622112-19

Burlev V.A.

Nauchnyĭ tsentr akusherstva, ginekologii i perinatologii im. akad. V.I. Kulakova, Moskva

Il'iasova N.A.

Nauchnyĭ tsentr akusherstva, ginekologii i perinatologii im. akad. V.I. Kulakova, Moskva

AlphaB-crystallin expression in eutopic and ectopic endometrium among the patients with peritoneal form endometriosis: pregnancy outcome

Authors:

Burlev V.A., Il'iasova N.A.

More about the authors

Journal: Russian Journal of Human Reproduction. 2016;22(1): 12‑19

DOI: 10.17116/repro201622112-19

Read: 735 times

Download PDF (RU)

Contact the author

Table of contents

To cite this article:

Burlev VA, Il'iasova NA. AlphaB-crystallin expression in eutopic and ectopic endometrium among the patients with peritoneal form endometriosis: pregnancy outcome. Russian Journal of Human Reproduction. 2016;22(1):12‑19. (In Russ.)
https://doi.org/10.17116/repro201622112-19

Подписка 2026 Проблемы репродукции (Russian Journal of Human Reproduction)

Использование инфографики авторами научных работ

Close metadata

Recommended articles:

Experience with the use of amitriptyline for chronic pelvic pain associated with endometriosis. Russian Journal of Human Reproduction. 2025;(1):86-93

Objectification of Pain in Patients with Endometriosis. (Literature review and own data). Russian Journal of Human Reproduction. 2025;(2):62-85

The role of macrophage polarization in the development of endometriosis and the therapeutic potential of its modulation. Russian Journal of Human Reproduction. 2025;(3):20-33

Visualization of the mitotic spindle in oocytes of patients with ovarian endometriosis as a prognostic factor for the effectiveness of fertilization and early embryonic development in IVF/ICSI programs. Russian Journal of Human Reproduction. 2025;(3):55-62

Influence of carriage of papillomavirus infection on the course of external genital endometriosis in patients with intraepithelial lesion of the cervix. Russian Journal of Human Reproduction. 2025;(3):77-83

The dependence of the ploidic status of embryos on the state of the spindle of oocyte division, assessed using polarization microscopy in IVF programs in women with ovarian endometriosis. Russian Bulletin of Obstetrician-Gynecologist. 2025;(1):44-49

Primary ovarian insufficiency: opportunities and prospects for the realization of reproductive function. Russian Bulletin of Obstetrician-Gynecologist. 2025;(2):42-47

The role of pelvic inflammatory diseases in reproductive function disorders in women. Russian Bulletin of Obstetrician-Gynecologist. 2025;(2):48-54

Characteristics of factors contributing to disorders of reproductive function in patients with adenomyosis. Russian Bulletin of Obstetrician-Gynecologist. 2025;(2):68-74

Use of gonadotropic releasing hormone agonists in hyperplastic syndrome in gynecology. Russian Bulletin of Obstetrician-Gynecologist. 2025;(4):51-58

Abstract:

Objectives. To study the alphaB-crystallin expression and angiogenic level in eutopic and ectopic endometrium in women with endometriosis and to estimate the influence of revealed changes on pregnancy. Material and methods. Immunohistochemistry was used to investigate the alphaB-crystallin expression and angiogenic level in eutopic and ectopic endometrium among the women with endometriosis. AlphaB-crystallin, VEGF-A, sVEGF R1 concentrations in blood and in peritoneal fluid ere measured with the help of ELISA. The pregnancy outcome in patients with endometriosis was accessed in 6 months after the end of the treatment. Immunohistochemistry and ELISA data were compared in women in patients with endometriosis and healthy women with and without pregnancy. Results. AlphaB-crystallin expression in glandular epithelium of eutopic endometrium was lower in patients with endometriosis without pregnancy compared with healthy women and patients with pregnancy. AlphaB-crystallin levels in patients with endometriosis and pregnancy were similar to healthy women. AlphaB-crystallin can be used as biomarker of endometrial receptivity in patients with endometriosis and predict pregnancy outcomes. Conclusion. AlphaB-crystallin level can be used for endometrial receptivity assessment and pregnancy outcomes prediction in patients with endometriosis.

Keywords:

endometriosis

infertility

eutopic endometrium

alphaB-crystallin

pregnancy outcome

Authors:

Burlev V.A.

Nauchnyĭ tsentr akusherstva, ginekologii i perinatologii im. akad. V.I. Kulakova, Moskva

Il'iasova N.A.

Nauchnyĭ tsentr akusherstva, ginekologii i perinatologii im. akad. V.I. Kulakova, Moskva

Close metadata

References:

Burlev VA. Proliferative and angiogenic activity in eutopic and ectopic endometrium in patients with peritoneal endometriosis. Problemy reproduktsii. 2006;1:78-87. (In Russ.). Available at: http://www.mediasphera.ru/journals/reproduction/183/eng/2704/ Accessed November 16,2015.
Burlev VA. Autoparacrine impairments of angiogenesis regulation in proliferative forms of female reproductive diseases. Obstetrics and Gynecology. 2006;3:34-40. (In Russ.). Available at: http://elibrary.ru/item.asp?id=9226321. Accessed November 16,2015.
Burlev VA. The role of soluble and cellular selectins in ligand-receptor interactions between the embryo and endometrium. Problemy reproduktsii. 2014;5:66-72. (In Russ.). Available at: http://www.mediasphera.ru/journals/reproduction/1283/eng/20959/ Accessed November 16,2015.
Burlev VA, Ilyasova NA. The density of nervous fiber and nucleus isoforms expression of estrogen and progesterone receptors in eutopic endometrium in patients with peritoneal endometriosis: pregnancy outcomes. Problemy reproduktsii. 2014;6:61-69. (In Russ.). doi: 10.17116/repro201420661-69.
Burlev VA, Ilyasova NA. Signaling LIF and IL-1 pathways changes in patients with peritoneal endometriosis and infertility. Problemy reproduktsii. 2015;4:99-109. (In Russ.). doi: 10.17116/repro201521499-109.
Burlev VA, Ilyasova NA. Cluster of signaling pathway LIF/LIFR/gp130 activation in embrios during IVF/ICSI. Problemy reproduktsii. 2015;2:8-13. (In Russ.). doi: 10.17116/repro20152128-13.
Burlev VA, Il'iasova NA, Dubinskaia ED, Gasparian SA, Gavrilova LV. Adamian LV. Pro- and antiangiogenic activity in patients with retrocervical endometriosis. Problemy reproduktsii. 2015;2:8-13. (In Russ). Available at: http://www.mediasphera.ru/journals/reproduction/178/2597/. Accessed November 16,2015.
Bourlev V, Volkov N, Pavlovitch S, Lets N, Larsson A, Olovsson M. The relationship between microvessel density, proliferative activity and expression of vascular endothelial growth factor-A and its receptors in eutopic endometrium and endometriotic lesions. Reproduction. 2006;132(3):501-509. doi: 10.1530/rep.1.01110.
Dimberg A, Rylova S, Dieterich LC, Olsson AK, Schiller P, Wikner C, Bohman S, Botling J, Lukinius A, Wawrousek EF, et al. alphaB-crystallin promotes tumor angiogenesis by increasing vascular survival during tube morphogenesis. Blood. 2008;111:2015-2023. doi: http://dx.doi.org/10.1182/blood-2007-04-087841.
Gruidl M, Buyuksal A, Babaknia A, Fazleabas AT, Sivarajah S, Satyaswaroop PG., Tabibzadeh S. The progressive rise in the expression of alpha crystallin B chain in human endometrium is initiated during the implantation window: modulation of gene expression by steroid hormones. Mol Hum Reprod. 1997;3:333-342. doi: 10.1093/molehr/3.4.333.
Kamradt MC, Chen F, Cryns VL. The small heat shock protein alpha B-crystallin negatively regulates cytochrome c- and caspase-8-dependent activation of caspase-3 by inhibiting its autoproteolytic maturation. J Biol Chem. 2001;276:16059-16063. doi: 10.1074/jbc.C100107200.
Kamradt MC, Lu M, Werner ME, Kwan T, Chen F, Strohecker A, Oshita S, Wilkinson JC, Yu C, Oliver PG, et al. The small heat shock protein alpha B-crystallin is a novel inhibitor of TRAIL-induced apoptosis that suppresses the activation of caspase-3. J Biol Chem. 2005;280:11059-11066. doi: 10.1074/jbc.M413382200.
MacIntyre DA, Tyson EK, Read M, Smith R, Yeo G, Kwek K, Chan EC. Contraction in human myometrium is associated with changes in small heat shock proteins. Endocrinology. 2008;149;245-252. doi: http://dx.doi.org/10.1210/en.2007-0662.
Moberg C, Bourlev V, Ilyasova N, Olovsson M. Levels of oestrogen receptor, progesterone receptor and αB-crystallin in eutopic endometrium in relation to pregnancy in women with endometriosis. Hum Fertil (Camb). 2014 Jun 19;1-8. doi: 10.3109/14647273.2014.922705.
Moberg С, Bourlev V, Ilyasova N, Olovsson M. Endometrial expression of LIF and its receptor and peritoneal fluid levels of IL-1a and IL-6 in women with endometriosis are associated with the probability of pregnancy. Arch Gynecol Obstet. 2015;292(2):429-437. doi: 10.1007/s00404-015-3626-0.
Narberhaus F. Alpha-crystallin-type heat shock proteins: socializing minichaperones in the context of a multichaperone network. Microbiol Mol Biol Rev. 2002;66:64-93. doi: 10.1128/MMBR.66.1.64-93.2002.
Revised American Society for Reproductive Medicine classification of endometriosis: 1996. Fertil Steril. 1997;67:817-821. doi: 10.1016/S0015-0282(97)81391-X.
Riesewijk A, Martin J, van Os R, Horcajadas JA, Polman J, Pellicer A, Mosselman S, Simon C. Gene expression profiling of human endometrial receptivity on days LH+2 versus LH+7 by microarray technology. Mol Hum Reprod .2003;9:253-264. doi: 10.1093/molehr/gag037.