Efficiency evaluation of the use of the stromal vascular fraction in facial surgery

Orlova Yu.M.; Manturova N.E.; Verbo E.V.; Ustyugov A.Yu.

doi:https://doi.org/10.17116/plast.hirurgia20210315

Orlova Yu.M.

Pirogov Russian National Research Medical University

ORCID: 0000-0003-0496-6008

Manturova N.E.

Pirogov Russian National Research Medical University;
Institute of Plastic Surgery and Cosmetology

SPIN RSCI: 5232-0412
Scopus AuthorID: 55461712100
ORCID: 0000-0003-4281-1947

Verbo E.V.

Petrovsky Russian Research Center of Surgery;
National Medical Research Center for Dentistry, Oral and Maxillofacial Surgery

ORCID: 0000-0001-9843-5026

Ustyugov A.Yu.

The Russian National Research Medical University named after N.I. Pirogov

ORCID: 0000-0002-4598-1194

Efficiency evaluation of the use of the stromal vascular fraction in facial surgery

Authors:

Orlova Yu.M., Manturova N.E., Verbo E.V., Ustyugov A.Yu.

More about the authors

Journal: Plastic Surgery and Aesthetic Medicine. 2021;(3): 5‑15

DOI: 10.17116/plast.hirurgia20210315

Read: 4382 times

To cite this article:

Orlova YuM, Manturova NE, Verbo EV, Ustyugov AYu. Efficiency evaluation of the use of the stromal vascular fraction in facial surgery. Plastic Surgery and Aesthetic Medicine. 2021;(3):5‑15. (In Russ., In Engl.)
https://doi.org/10.17116/plast.hirurgia20210315

Подписка 2026 Пластическая хирургия и эстетическая медицина (Plastic Surgery and Aesthetic Medicine)

Использование инфографики авторами научных работ

Close metadata

OBJECTIVE

To evaluate the clinical efficacy of the stromal vascular fraction (SVF), its biological properties, to determine the factors influencing the biological properties of SVF.

MATERIAL AND METHODS

We analyzed clinical efficacy of SVF, its biological properties and factors influencing these properties. SVF has a positive effect on fatty autograft engraftment. Better skin quality was noted in some cases. We distinguished the type of cells in SVF (adipose derived stem cells — ADSCs) determining clinical efficacy of SVF. We found positive correlation between proliferative potential of SVF mesenchymal cells and thickness of subcutaneous fat layer over time. No dependence of total number of SVF cells from this parameter was noted. Both local factors and patient characteristics influence proliferative potential of SVF mesenchymal cells.

RESULTS

The authors analyzed own results, presented hypotheses about the mechanism of SVF influence on adipose graft engraftment and role of microenvironment in homeostasis of adipose tissue.

Keywords:

lipofilling

rhytidectomy

cicatricial deformities

stromal vascular fraction

microenvironment

adipose derived stem cells

regenerative medicine

Authors:

Orlova Yu.M.

Pirogov Russian National Research Medical University

ORCID: 0000-0003-0496-6008

Manturova N.E.

Pirogov Russian National Research Medical University;
Institute of Plastic Surgery and Cosmetology

SPIN RSCI: 5232-0412
Scopus AuthorID: 55461712100
ORCID: 0000-0003-4281-1947

Verbo E.V.

Petrovsky Russian Research Center of Surgery;
National Medical Research Center for Dentistry, Oral and Maxillofacial Surgery

ORCID: 0000-0001-9843-5026

Ustyugov A.Yu.

The Russian National Research Medical University named after N.I. Pirogov

ORCID: 0000-0002-4598-1194

Received:

30.04.2021

Accepted:

15.05.2021

Close metadata

References:

Krastev TK, Schop SJ, Hommes J, Piatkowski A, van der Hulst RRWJ. Autologous fat transfer to treat fibrosis and scar-related conditions: a systematic review and meta-analysis. Journal of Plastic, Reconstructive & Aesthetic Surgery. 2020 73(11):2033-2048. Published: August 20, 2020. https://doi.org/10.1016/j.bjps.2020.08.023
Kerastem Technologies, LLC. STYLE — A Trial of Cell Enriched Adipose For Androgenetic Alopecia (STYLE). In: ClinicalTrials.gov [Internet]. Bethesda (MD): National Library of Medicine (US). First Posted July 21, 2015. Accessed April 19, 2021. https://clinicaltrials.gov/ct2/show/NCT02503852
Bank J, Fuller SM, Henry GI, Zachary LS. Fat grafting to the hand in patients with Raynaud phenomenon: a novel therapeutic modality. Plast Reconstr Surg. 2014;133(5):1109-1118.
Granel B, Daumas A, Jouve E, Harlé J-R, Nguyen P-S, Chabannon C, et al. Safety, tolerability and potential efficacy of injection of autologous adipose-derived stromal vascular fraction in the fingers of patients with systemic sclerosis: an open-label phase I trial. Ann Rheum Dis. 2015;74:2175-2182. First published August 11, 2014.
Masgutov RF, Salikhov RZ, Rizvanov AA, Salafutdinov II, Bogov AA, Plakseichuk YuA. Primenenie kletok stromal’noj vaskulyarnoj fraktsii zhirovoj tkani pri lechenii polnoslojnogo defekta gialinovogo khryashcha medial’nogo myshchelka bedrennoj kosti kolennogo sustava: klinicheskij sluchaj. Geny i kletki. 2014;IX(3):303-306. (In Russ.). Accessed April 19, 2021. https://cyberleninka.ru/article/n/primenenie-kletok-stromalnoy-vaskulyarnoy-fraktsii-zhirovoy-tkani-pri-lechenii-polnosloynogo-defekta-gialinovogo-hryascha-medialnogo
Coleman SR. Structural fat grafting: more than a permanent filler. Plast Reconstr Surg. 2006;118:108-120.
Xie Y, Zheng DN, Li QF, et al. An integrated fat grafting technique for cosmetic facial contouring. J Plast Reconstr Aesthet Surg. 2010;63:270-276.
Gutowski CA. Current applications and safety of autologous fat grafts: a report of the ASPS fat graft task force. Plast Reconstr Surg. 2009;124:272-280.
Yoshimura K, Sato K, Aoi N, et al. Cell-assisted lipotransfer for facial lipoatrophy: efficacy of clinical use of ADSCs. Dermatol Surg. 2008;34: 1178-1185.
Charles-de-Sa L, Gontijo-de-Amorim NF, Takiya CM, et al. Antiaging treatment of the facial skin by fat graft and adipose-derived stem cells. Plast Reconstr Surg. 2015;135(4):999-1009.
Gentile P, De Angelis B, Pasin M, et al. Adipose-derived stromal vascular fraction cells and platelet-rich plasma: basic and clinical evaluation for cell-based therapies in patients with scars on the face. J Craniofac Surg. 2014; 25(1):267-272. https://doi.org/10.1097/01.scs.0000436746.21031.ba
Gentile P, De Angelis B, Pasin M, Cervelli G, Curcio CB, Floris M, Di Pasquali C, Bocchini I, Balzani A, Nicoli F, Insalaco C, Tati E, Lucarini L, Palla L, Pascali M, De Logu P, Di Segni C, Bottini DJ, Cervelli V. Adipose-derived stromal vascular fraction cells and platelet-rich plasma: basic and clinical evaluation for cell-based therapies in patients with scars on the face. J Craniofac Surg. 2014;25(1):267-272. PMID: 24406591. https://doi.org/10.1097/01.scs.0000436746.21031.ba
Sasaki GH. The Safety and Efficacy of Cell-Assisted Fat Grafting to Traditional Fat Grafting in the Anterior Mid-Face: An Indirect Assessment by 3D Imaging. Aesthetic Plast Surg. 2015;39(6):833-846. Epub 2015 Sept 03. PMID: 26335660. https://doi.org/10.1007/s00266-015-0533-5
Schendel SA. Enriched autologous facial fat grafts in aesthetic surgery: 3D volumetric results. Aesthet Surg J. 2015;35(8):913-919. Epub 2015 Aug 04. PMID: 26242853. https://doi.org/10.1093/asj/sjv140
Gontijo-de-Amorim NF, Charles-de-Sá L, Rigotti G. Fat Grafting for Facial Contouring Using Mechanically Stromal Vascular Fraction-Enriched Lipotransfer. Clin Plast Surg. 2020;47(1):99-109. Epub 2019 Oct 25. PMID: 31739903. https://doi.org/10.1016/j.cps.2019.08.012
Grishchenko SV, Nadtochy AG, Malitskaya OA. Monitoring sostoyaniya tkanej shchechno-skulovoj oblasti posle lipofilinga na etapakh reabilitatsii. Vestnik vosstanovitel’noj meditsiny. 2015;2:7-12. (In Russ.).
Tsekouras A, Mantas D, Tsilimigras DI, Moris D, Kontos M, Zografos GC. Comparison of the Viability and Yield of Adipose-Derived Stem Cells (ASCs) from Different Donor Areas. In Vivo. 2017;31(6):1229-1234. https://doi.org/10.21873/invivo.11196
Arias JI, Aller MA, Arias J. Surgical inflammation: a pathophysiological rainbow. J Transl Med. 2009;7:19. Published 2009 Mar 23. https://doi.org/10.1186/1479-5876-7-19
Arias J, Aller MA, Arias JI, Lorente L. Traumatismos mecánicos: Contusión. Herida. Fractura. In: Arias J, Aller MA, Arias JI, Lorente L, eds. Fisiopatología Quirúrgica. Chapter 2. Madrid: Ed. Tébar; 1999;35-49.
Rosser BG, Gores GJ. Liver cell necrosis: Cellular mechanisms and clinical implications. Gastroenterology. 1995;108:252-275.
Jackson RM, Ann HS, Oparil S. Hypoxia-induced oxygen tolerante:maintenance of endotelial metabolic function. Exp Lung Res. 1988;14:887-896.
Gimble JM, Katz AJ, Bunnell BA. Adipose-derived stem cells for regenerative medicine. Circ Res. 2007;100:1249-1260.
Cawthorn WP, Scheller EL, MacDougald OA. Adipose tissue stem cells: the great WAT hope. Trends Endocrinol Metab. 2012;23:270-277.
Serena C, Keiran N, Ceperuelo-Mallafre V, et al. Obesity and type 2 diabetes alters the immune properties of human adipose derived stem cells. Stem Cells. 2016;34:2559-2573.
Silva KR, Liechocki S, Carneiro JR, et al. Stromal-vascular fraction content and adipose stem cell behavior are altered in morbid obese and post bariatric surgery ex-obese women. Stem Cell Res Ther. 2015;6:72.
Badimon L, Cubedo J. Adipose tissue depots and in- flammation: effects on plasticity and resident mesenchymal stem cell function. Cardiovasc Res. 2017;113:1064-1073.
Palmer AK, Kirkland JL. Aging and adipose tissue: potential interventions for diabetes and regenerative medicine. Exp Gerontol. 2016;86:97-105.
Festa E, et al. Adipocyte lineage cells contribute to the skin stem cell niche to drive hair cycling. Cell. 2011;146:761-771.
Li H, Xu Y, Fu Q, et al. Effects of multiple agents on epithelial differentiation of rabbit adipose-derived stem cells in 3D culture. Tissue Eng Part A. 2012;18(17-18):1760-1770.
Derby BM, Dai H, Reichensperger J, Cox L, Harrison C, Cosenza N, Neumeister MW. Adipose-Derived Stem Cell to Epithelial Stem Cell Transdifferentiation: A Mechanism to Potentially Improve Understanding of Fat Grafting’s Impact on Skin Rejuvenation. Aesthetic Surgery Journal. 2013; 34(1):142-153.