Objective: to evaluate the efficiency of ultrasound-guided multifocal core needle biopsy in the complex diagnosis of lymphoma. Subjects and methods. From January 2015 to June 2016, 89 patients with suspected lymphoma underwent ultrasound-guided multifocal core needle biopsy from the most accessible suspicious focus, followed by a morphological examination. Specimens were obtained from the altered peripheral lymph nodes (LNs) in 50 patients, from the mediastinum in 14, from retroperitoneal LNs in 13, from chest wall infiltration in 4, from the liver in 2, from a soft-tissue component of bone tumor in 2, from the small pelvis in 2, from the breast in 1, and from the testicle in 1. Results. Core needle biopsy, followed by a morphological examination, could establish the diagnosis in 86 (96.6%) patients. The latter could not be made after core needle biopsy in 3 (3.4%) cases; excisional LN biopsy was required. The diagnosis of lymphoma was morphologically verified in 80 (93%) patients; another diagnosis different from that of lymphoma was established in 6 (7%) patients. Conclusion. Ultrasound-guided multifocal core needle biopsy is a highly effective and safe technique to obtain specimens for morphological examinations in the diagnosis of lymphoma.