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Melkumyan A.R.

Ryzhikh National Medical Research Center of Coloproctology

Chistyakova D.A.

Ryzhikh National Medical Research Center of Coloproctology

Shafikova A.A.

Ryzhikh National Medical Research Center of Coloproctology

Spivak M.V.

Ryzhikh National Medical Research Center of Coloproctology

Achkasov S.I.

Ryzhikh National Medical Research Center of Coloproctology

Antibiotic resistance of obligate anaerobic bacteria and the role of molecular methods in their study

Authors:

Melkumyan A.R., Chistyakova D.A., Shafikova A.A., Spivak M.V., Achkasov S.I.

More about the authors

Journal: Laboratory Service. 2024;13(1): 60‑66

Read: 1593 times


To cite this article:

Melkumyan AR, Chistyakova DA, Shafikova AA, Spivak MV, Achkasov SI. Antibiotic resistance of obligate anaerobic bacteria and the role of molecular methods in their study. Laboratory Service. 2024;13(1):60‑66. (In Russ.)
https://doi.org/10.17116/labs20241301160

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References:

  1. Lassmann B, Gustafson DR, Wood CM, Rosenblatt JE. Reemergence of anaerobic bacteremia. Clinical Infectious Diseases. 2007;44(7):895-900. 
  2. Schuetz AN. Antimicrobial resistance and susceptibility testing of anaerobic bacteria. Clinical Infectious Diseases. 2014;59(5):698-705. 
  3. Nagy E, Urbán E, Nord CE; ESCMID Study Group on Antimicrobial Resistance in Anaerobic Bacteria. Antimicrobial susceptibility of Bacteroides fragilis group isolates in Europe: 20 years of experience. Clinical Microbiology and Infection. 2011;17(3):371-379. 
  4. Wright GD. The antibiotic resistome: the nexus of chemical and genetic diversity. Nature Reviews Microbiology. 2007;5(3):175-186. 
  5. Salyers AA, Gupta A, Wang Y. Human intestinal bacteria as reservoirs for antibiotic resistance genes. Trends in microbiology. 2004;12:9:412-416. 
  6. Boente RF, Ferreira LQ, Falcão LS, et al. Detection of resistance genes and susceptibility patterns in Bacteroides and Parabacteroides strains. Anaerobe. 2010;16(3):190-194. 
  7. Snydman DR, Cuchural GJ Jr, McDermott L, Gill M. Correlation of various in vitro testing methods with clinical outcomes in patients with Bacteroides fragilis group infections treated with cefoxitin: A retrospective analysis. Antimicrobial Agents and Chemotherapy. 1992;36(3):540-544. 
  8. Dubreuil L, Veloo AC, Sóki J; ESCMID Study Group for Anaerobic Infections (ESGAI). Correlation between antibiotic resistance and clinical outcome of anaerobic infections; mini-review. Anaerobe. 2021;72:102463.
  9. Chow AW, Guze LB. Bacteroidaceae bacteremia: clinical experience with 112 patients. Medicine. 1974;53(2):93-126. 
  10. McNamara MJ, Pasquale MD, Evans SR. Acute appendicitis and the use of intraperitoneal cultures. Surgery, Gynecology & Obstetrics. 1993;177(4):393-397. 
  11. Hopkins JA, Lee JC, Wilson SE. Susceptibility of intra-abdominal isolates at operation: A predictor of postoperative infection. The American Surgeon. 1993;59(12):791-796. 
  12. Mosdell DM, Morris DM, Voltura A, et al. Antibiotic treatment for surgical peritonitis. Annals of Surgery. 1991;214(5):543-549. 
  13. Dougherty SH, Saltzstein EC, Peacock JB, Mercer LC, Cano P. Perforated or gangrenous appendicitis treated with aminoglycosides: how do bacterial cultures influence management? Archives of Surgery. 1989;124(11):1280-1283.
  14. Fang H, Li X, Yan M-K, et al. Antimicrobial susceptibility of Bacteroides fragilis group organisms in Hong Kong, 2020-2021. Anaerobe. 2023;82:102756.
  15. Píriz S, Vadillo S, Quesada A, et al. Relationship between penicillin-binding protein patterns and β-lactamases in clinical isolates of Bacteroides fragilis with different susceptibility to β-lactam antibiotics. Journal of Medical Microbiology. 2004;53(3):213-221. 
  16. Ezeji JC, Sarikonda DK, Hopperton A, et al. Parabacteroides distasonis: intriguing aerotolerant gut anaerobe with emerging antimicrobial resistance and pathogenic and probiotic roles in human health. Gut Microbes. 2021;13(1):1922241.
  17. Sherwood JE, Fraser S, Citron DM, et al. Multi-drug resistant Bacteroides fragilis recovered from blood and severe leg wounds caused by an improvised explosive device (IED) in Afghanistan. Anaerobe. 2011;17(4):152-155. 
  18. Yekani M, Rezaee MA, Beheshtirouy S, et al. Carbapenem resistance in Bacteroides fragilis: A review of molecular mechanisms. Anaerobe. 2022;76:102606.
  19. Solomkin JS, Mazuski JE, Bradley JS, et al. Diagnosis and management of complicated intra-abdominal infection in adults and children: guidelines by the Surgical Infection Society and the Infectious Diseases Society of America. Surgical Infections. 2010;11(1):79-109. 
  20. Nagy E, Urbán E, Nord CE; ESCMID Study Group on Antimicrobial Resistance in Anaerobic Bacteria. Antimicrobial susceptibility of Bacteroides fragilis group isolates in Europe: 20 years of experience. Clin Microbiol Infect. 2011;17(3):371-379. 
  21. Gal M, Brazier JS. Metronidazole resistance in Bacteroides spp. carrying nim genes and the selection of slow-growing metronidazole-resistant mutants. Journal of Antimicrobial Chemotherapy. 2004;54(1):109-116. 
  22. Snydman DR, Jacobus NV, McDermott LA, et al. Update on resistance of Bacteroides fragilis group and related species with special attention to carbapenems 2006-2009. Anaerobe. 2011;17(4):147-151. 
  23. Niestępski S, Harnisz M, Korzeniewska E, et al. The emergence of antimicrobial resistance in environmental strains of the Bacteroides fragilis group. Environment international. 2019;124:408-419. 
  24. Bahar H, Torun MM, Demirci M, Kocazeybek B. Antimicrobial resistance and β-lactamase production of clinical isolates of Prevotella and Porphyromonas species. Chemotherapy. 2005;51(1):9-14. 
  25. Könönen E, Conrads G, Nagy E. Bacteroides, Porphyromonas, Prevotella, Fusobacterium, and Other Anaerobic Gram‐Negative Rods. Manual of Clinical Microbiology. 2015:967-993. 
  26. Brook I, Wexler HM, Goldstein EJC. Antianaerobic antimicrobials: spectrum and susceptibility testing. Clinical Microbiology Reviews. 2013;26(3):526-546. 
  27. Buboltz JB, Murphy-Lavoie HM. Gas gangrene. StatPearls [Internet]. StatPearls Publishing; 2022.
  28. Murray R, Rosenthal S, Pfaller A. Medical Microbiology. 9th ed. Elsevier Health Sciences: Philadelphia, PA, USA; 2020.
  29. Tansuphasiri U, Matra W, Sangsuk L. Antimicrobial resistance among Clostridium perfringens isolated from various sources in Thailand. Southeast Asian J Trop Med Public Health. 2005 July; 36(4):954-961. 
  30. Banawas SS. Systematic review and meta-analysis on the frequency of antibiotic-resistant clostridium species in Saudi Arabia. Antibiotics. 2022;11(9):1165.
  31. Johansson Å, Greko C, Engström BE, Karlsson M. Antimicrobial susceptibility of Swedish, Norwegian and Danish isolates of Clostridium perfringens from poultry, and distribution of tetracycline resistance genes. Veterinary Microbiology. 2004;99(3-4):251-257. 
  32. Hecht DW. Anaerobes: antibiotic resistance, clinical significance, and the role of susceptibility testing. Anaerobe. 2006;12(3):115-121. 
  33. Camacho N, Espinoza C, Rodríguez C, Rodríguez E. Isolates of Clostridium perfringens recovered from Costa Rican patients with antibiotic-associated diarrhoea are mostly enterotoxin-negative and susceptible to first-choice antimicrobials. Journal of Medical Microbiology. 2008;57(3):343-347. 
  34. Akhi MT, Bidar Asl S, Pirzadeh T, et al. Antibiotic sensitivity of Clostridium perfringens isolated from faeces in Tabriz, Iran. Jundishapur Journal of Microbiology. 2015;8(7):e20863.
  35. Ob utverzhdenii sanitarnykh pravil i norm SanPiN 3.3686-21 «Sanitarno-epidemiologicheskie trebovaniya po profilaktike infektsionnykh boleznej» ot 28 yanvarya 2021. docs.cntd.ru [Elektronnyj resurs]. (In Russ.). Accessed November 14, 2021. https://docs.cntd.ru/document/573660140
  36. Pike CM, Theriot CM. Mechanisms of colonization resistance against Clostridioides difficile. The Journal of Infectious Diseases. 2021;223(Suppl 3):S194-S200.
  37. Goudarzi M, Seyedjavadi SS, Goudarzi H, et al. Clostridium difficile infection: epidemiology, pathogenesis, risk factors, and therapeutic options. Scientifica. 2014;2014:916826.
  38. Evans CT, Safdar N. Current trends in the epidemiology and outcomes of Clostridium difficile infection. Clinical Infectious Diseases. 2015;60(Suppl 2):S66-S71. 
  39. Dmitrieva NV, Klyasova GA, Bakulina NV, et al. A prevalence of Clostridium difficile-associated diarrhea in hospitalized patients (results of a Russian prospective multicenter study). Klinicheskaya mikrobiologiya i antimikrobnaya khimioterapiya. 2017;19(4):268-274. (In Russ.).
  40. Shelygin YuA, Aleshkin VA, Sukhina MA, et al. Clinical Recommendations of the National Association of Specialists for the Healthcare-Related Infections Control and the Russian Association of Coloproctology on Diagnosis, Treatment and Prophylaxis of Clostridium Difficile-Associated Diarrhea (CDI). Koloproktologiya. 2018;3:7-23. (In Russ.).
  41. Hecht DW, Galang MA, Sambol SP, et al. In vitro activities of 15 antimicrobial agents against 110 toxigenic Clostridium difficile clinical isolates collected from 1983 to 2004. Antimicrobial Agents and Chemotherapy. 2007;51(8):2716-2719.
  42. Spigaglia P, Barbanti F, Mastrantonio P; European Study Group on Clostridium difficile (ESGCD). Multidrug resistance in European Clostridium difficile clinical isolates. Journal of Antimicrobial Chemotherapy. 2011;66(10):2227-2234.
  43. Peláez T, Alcalá L, Alonso R, et al. Reassessment of Clostridium difficile susceptibility to metronidazole and vancomycin. Antimicrobial Agents and Chemotherapy. 2002;46(6):1647-1650.
  44. Tkhawkho L, Nitzan O, Pastukh N, et al. Antimicrobial susceptibility of Clostridium difficile isolates in Israel. Journal of global Antimicrobial Resistance. 2017;10:161-164. 
  45. Goldstein EJC, Citron DM, Merriam CV, et al. Comparative in vitro activities of XRP 2868, pristinamycin, quinupristin-dalfopristin, vancomycin, daptomycin, linezolid, clarithromycin, telithromycin, clindamycin, and ampicillin against anaerobic gram-positive species, actinomycetes, and lactobacilli. Antimicrobial Agents and Chemotherapy. 2005;49(1):408-413. 
  46. Nagy E. Anaerobic infections: update on treatment considerations. Drugs. 2010;70:841-858. 
  47. Veloo ACM, Welling GW, Degener JE. Antimicrobial susceptibility of clinically relevant Gram-positive anaerobic cocci collected over a three-year period in the Netherlands. Antimicrobial Agents and Chemotherapy. 2011;55(3):1199-1203.
  48. International Organization for Standardization (ISO). Clinical Laboratory Testing and In Vitro Diagnostic Test Systems-Susceptibility Testing of Infectious Agents and Evaluation of Performance of Antimicrobial Susceptibility Test Devices: Reference Method for Testing the In Vitro Activity of Antimicrobial Agents Against Rapidly Growing Aerobic Bacteria Involved in Infectious Diseases. ISO, 2006.
  49. Opredelenie chuvstvitel’nosti mikroorganizmov k antimikrobnym preparatam. Klinicheskie rekomendatsii. Versiya 2021-01. 222 s. (In Russ.).
  50. Kuzmenkov AYu, Vinogradova AG. Antimicrobial resistance monitoring: a review of information resources. Bulletin of Siberian Medicine. 2020;19(2):163-170. 
  51. Ferreira L, Sánchez-Juanes F, Porras-Guerra I, et al. Microorganisms direct identification from blood culture by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Clinical Microbiology and Infection. 2011;17(4):546-551. 
  52. Barnini S, Ghelardi E, Brucculeri V, Morici P, Lupetti A. Rapid and reliable identification of Gram-negative bacteria and Gram-positive cocci by deposition of bacteria harvested from blood cultures onto the MALDI-TOF plate. BMC Microbiology. 2015;15:124. 
  53. Florio W, Tavanti A, Ghelardi E, Lupetti A. MALDI-TOF MS applications to the detection of antifungal resistance: State of the art and future perspectives. Frontiers in Microbiology. 2018;9:2577.
  54. Priputnevich TV, Ilina EN, Kafarskaya LI, et al. Matrichno-aktivirovannaya lazernaya desorbtsionnaya/ionizatsionnaya vremyaproletnaya mass-spektrometriya (MALDI-TOF MS) v klinicheskoj mikrobiologii. M. 2020:60. (In Russ.).
  55. Melkumyan AR, Shiyan OV, Mitichkin AE, et al. Primenenie mass-spektrometricheskogo metoda tipirovaniya vysokovirulentnykh klonov streptokokka gruppy B v prognozirovanii riska razvitiya neonatal’nykh infektsij. Akusherstvo i ginekologiya. Novosti. Mneniya. Obuchenie. 2020;8(1/27):98. (In Russ.).
  56. Nagy E, Becker S, Sóki J, Urbán E, Kostrzewa M. Differentiation of division I (cfiA-negative) and division II (cfiA-positive) Bacteroides fragilis strains by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Journal of Medical Microbiology. 2011;60(11):1584-1590.
  57. Johansson Å, Nagy E, Sóki J; ESGAI (ESCMID Study Group on Anaerobic Infections). Detection of carbapenemase activities of Bacteroides fragilis strains with matrix-assisted laser desorption ionization — time of flight mass spectrometry (MALDI-TOF MS). Anaerobe. 2014;26:49-52. 

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