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Chebotar' I.V.

GOU VPO Nizhegorodskaia gosudarstvennaia meditsinskaia akademiia Minzdravsotsrazvitiia RF, Nizhniĭ Novgorod

Polikarpova S.V.

Association of laboratory specialists and organizations «Federation of Laboratory Medicine»;
Moscow City Hospital №15 named after O.M. Filatov

Bocharova Yu.A.

National Medical Research Center of Children’s Health, Moscow, Russia

Mayansky N.A.

National Medical Research Center of Children’s Health, Moscow, Russia

Use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for identification of bacteria and fungi of the pathogenicity group III and IV

Authors:

Chebotar' I.V., Polikarpova S.V., Bocharova Yu.A., Mayansky N.A.

More about the authors

Journal: Laboratory Service. 2018;7(2): 78‑86

DOI: 10.17116/labs20187278-86

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USE OF MATRIX-ASSISTED LASER DESORPTION/IONIZATION TIME-OF-FLIGHT MASS SPECTROMETRY (MALDI-TOF MS) FOR IDENTIFICATION OF BACTERIA AND FUNGI OF THE PATHOGENICITY GROUP III AND IV
USE OF MATRIX-ASSISTED LASER DESORPTION/IONIZATION TIME-OF-FLIGHT MASS SPECTROMETRY (MALDI-TOF MS) FOR IDENTIFICATION OF BACTERIA AND FUNGI OF THE PATHOGENICITY GROUP III AND IV

To cite this article:

Chebotar' IV, Polikarpova SV, Bocharova YuA, Mayansky NA. Use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for identification of bacteria and fungi of the pathogenicity group III and IV. Laboratory Service. 2018;7(2):78‑86. (In Russ.)
https://doi.org/10.17116/labs20187278-86

 
 
Подписка 2025-2 (Laboratory Service)
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This document provides information on one of the promising technologies for taxonomic identification of microorganisms of the pathogenicity group III and IV, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), including methodological and organizational demands for correct and safe execution of this diagnostic method.

Keywords:

microbiology
identification
diagnostics
bacteria
micromycetes
matrix-assisted laser desorption ionization mass spectrometry

Authors:

Chebotar' I.V.

GOU VPO Nizhegorodskaia gosudarstvennaia meditsinskaia akademiia Minzdravsotsrazvitiia RF, Nizhniĭ Novgorod

Polikarpova S.V.

Association of laboratory specialists and organizations «Federation of Laboratory Medicine»;
Moscow City Hospital №15 named after O.M. Filatov

Bocharova Yu.A.

National Medical Research Center of Children’s Health, Moscow, Russia

Mayansky N.A.

National Medical Research Center of Children’s Health, Moscow, Russia

List of references:

  1. European Centre for Disease Prevention and Control (ECDC). http://ecdc.europa.eu (Accessed September 2015).
  2. Бочарова Ю.А., Чеботарь И.В., Маянский Н.А. Возможности, проблемы и перспективы масс-спектрометрических технологий в медицинской микробиологии. Клиническая лабораторная диагностика. 2016;61(4):249-256. [ Bocharova YuA, Chebotar IV, Mayanskii NA. The possibilities, problems and perspectives of mass-spectrometry in medical microbiology: publications review. Klin Lab Diagnost. 2016;61(4): 249-256. (In Russ.)]. https://doi.org/10.18821/0869-2084-2016-61-4-249-256
  3. Angeletti S. Matrix assisted laser desorption time of flight mass spectrometry (MALDI-TOF MS) in clinical microbiology. Journal of microbiological methods. 2017;138:20-29. https://doi.org/10.1016/j.mimet.2016.09.003
  4. Patel R. MALDI-TOF MS for the diagnosis of infectious diseases. Clin Chem. 2015;61(1):100-110. https://doi.org/10.1373/clinchem.2014.221770
  5. Chen JH, Ho PL, Kwan GS, She KK, Siu GK, Cheng VC, Yuen KY, Yam WC. Direct bacterial identification in positive blood cultures by use of two commercial matrix-assisted laser desorption ionization — time of flight mass spectrometry systems. J Clin Microbiol. 2013;51(6):1733-1739. https://doi.org/10.1128/JCM.03259-12
  6. Ferreira L, Sánchez-Juanes F, González-Avila M, Cembrero-Fuciños D, Herrero-Hernández A, González-Buitrago JM, Muñoz-Bellido JL. Direct identification of urinary tract pathogens from urine samples by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol. 2010;48(6):2110-2115. https://doi.org/10.1128/JCM.02215-09
  7. Segawa S, Sawai S, Murata S, Nishimura M, Beppu M, Sogawa K, Watanabe M, Satoh M, Matsutani T, Kobayashi M, Iwadate Y, Kuwabara S, Saeki N, Nomura F. Direct application of MALDI-TOF mass spectrometry to cerebrospinal fluid for rapid pathogen identification in a patient with bacterial meningitis. Clin Chim Acta. 2014;435:59-61. https://doi.org/10.1016/j.cca.2014.04.024
  8. Lau AF, Drake SK, Calhoun LB, Henderson CM, Zelazny AM. Development of a clinically comprehensive database and a simple procedure for identification of molds from solid media by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol. 2013;51(3):828-834. https://doi.org/10.1128/JCM.02852-12

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