Influence of connective tissue differentiation on scar tissue formation in children

Minaev S.V.; Grigorova A.N.; Vladimirova O.V.; Timofeev S.I.; Sirak A.G.; Vladimirov V.I.; Pogosyan A.A.; Zelenskaya M.V.

doi:https://doi.org/10.17116/hirurgia202105172

Minaev S.V.

Stavropol State Medical University

Grigorova A.N.

Stavropol State Medical University

Vladimirova O.V.

Stavropol State Medical University

Timofeev S.I.

Magadan Regional Children’s Hospital

Sirak A.G.

Stavropol State Medical University

Vladimirov V.I.

Pyatigorsk Interdistrict Oncology Dispensary

Pogosyan A.A.

Stavropol State Medical University

Zelenskaya M.V.

Stavropol State Medical University

Influence of connective tissue differentiation on scar tissue formation in children

Authors:

Minaev S.V., Grigorova A.N., Vladimirova O.V., Timofeev S.I., Sirak A.G., Vladimirov V.I., Pogosyan A.A., Zelenskaya M.V.

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Journal: Pirogov Russian Journal of Surgery. 2021;(5): 72‑77

DOI: 10.17116/hirurgia202105172

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To cite this article:

Minaev SV, Grigorova AN, Vladimirova OV, et al. . Influence of connective tissue differentiation on scar tissue formation in children. Pirogov Russian Journal of Surgery. 2021;(5):72‑77. (In Russ.)
https://doi.org/10.17116/hirurgia202105172

Подписка 2026 Хирургия. Журнал им. Н.И. Пирогова (Pirogov Russian Journal of Surgery)

Использование инфографики авторами научных работ

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OBJECTIVE

To study the immunohistochemical features of various scar tissues in children without connective tissue pathology and with undifferentiated connective tissue dysplasia syndrome.

MATERIAL AND METHODS

Tissue biopsy was performed in 217 children who underwent surgical treatment for various lesions, such as injuries, burns, as well as other procedures. There were 127 boys (58.5%) and 90 (41.5%) girls. The main group consisted of 98 (48.2%) children with scar tissue; group of UCTD syndrome — 65 (30.0%) children; control group — 43 (24.8%) patients without pathological scars. Histological examination of scar tissue and intact skin was carried out during primary or redo reconstructive surgery. Immunohistochemical study of antibodies against CD34, CD105, CD140b, PDGFs, COL types I, III and IV was performed.

RESULTS

The study showed a quantitative characteristic of expression of COL type I in hypertrophic scar with predominance in the main group (77.5±5.4%; p<0.05), and decrease in COL type IV. Keloid form was associated with predominance of granulation tissue in all layers of dermis and high levels of all types of collagen. In the group of UCTD, COL type III prevailed in all pathological forms of the scar. We determined quantitative indicators of expression of vascularization factors (CD34; CD105) and fibroblastic activity (CD140b; PDGFs).

CONCLUSION

Understanding the process of fibrinogenesis and analysis of stages of triggering mechanisms are essential for development of preventive algorithms. Individualized approach should be considered in the treatment. These studies are especially important in children with UCTD syndrome as high-risk group for pathological scarring. Thus, further research is required.

Keywords:

scars

scar tissue

collagen fibers

immunohistochemistry

children

Authors:

Minaev S.V.

Stavropol State Medical University

Grigorova A.N.

Stavropol State Medical University

Vladimirova O.V.

Stavropol State Medical University

Timofeev S.I.

Magadan Regional Children’s Hospital

Sirak A.G.

Stavropol State Medical University

Vladimirov V.I.

Pyatigorsk Interdistrict Oncology Dispensary

Pogosyan A.A.

Stavropol State Medical University

Zelenskaya M.V.

Stavropol State Medical University

Received:

27.05.2020

Accepted:

10.06.2020

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References:

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