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Gorbunov A.S.

Cardiology Research Institute, Tomsk National Research Medical Center

Sidorova M.V.

Chazov National Medical Research Center of Cardiology

Palkeeva M.E.

Chazov National Medical Research Center of Cardiology

Molokoedov A.S.

Chazov National Medical Research Center of Cardiology

Pisarenko O.I.

Chazov National Medical Research Center of Cardiology

Exogenous GalR2 receptor agonist reduces reperfusion injury in isolated rat heart

Authors:

Gorbunov A.S., Sidorova M.V., Palkeeva M.E., Molokoedov A.S., Pisarenko O.I.

More about the authors

Journal: Russian Cardiology Bulletin. 2024;19(3): 37‑42

DOI: 10.17116/Cardiobulletin20241903137

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Table of contents

EXOGENOUS GALR2 RECEPTOR AGONIST REDUCES REPERFUSION INJURY IN ISOLATED RAT HEART
EXOGENOUS GALR2 RECEPTOR AGONIST REDUCES REPERFUSION INJURY IN ISOLATED RAT HEART

To cite this article:

Gorbunov AS, Sidorova MV, Palkeeva ME, Molokoedov AS, Pisarenko OI. Exogenous GalR2 receptor agonist reduces reperfusion injury in isolated rat heart. Russian Cardiology Bulletin. 2024;19(3):37‑42. (In Russ.)
https://doi.org/10.17116/Cardiobulletin20241903137

Подписка 2025-2 (Russian Cardiology Bulletin)
 
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OBJECTIVE

To study the effect of exogenous GalR2 receptor agonist (peptide H-Trp-Thr-Leu-Asn-Ser-Ala-Gly-Tyr-Leu-Leu-Gly-Pro-βAla-His-OH) (G) on reperfusion injury after total ischemia in isolated rat heart model.

MATERIAL AND METHODS

Isolated hearts of Wistar rats perfused with Krebs-Henseleit solution (KHS) according to the Langendorff method were subjected to total normothermic ischemia and reperfusion. In the group G, rat heart was reperfused by KHS containing peptide 5 μg/ml within 10 min. No peptide was infused for further 20min reperfusion. Reperfusion by KHS was carried out in the control group. We estimated recovery of cardiac function and necrotic damage to cardiomyocytes.

RESULTS

Recovery of left ventricle pressure, heart rate, maximum rate of left ventricular contraction and relaxation (+dP/dt and — dP/dt, respectively) was more effective under the influence of G compared to the control group (p<0.05—0.01). End-diastolic pressure significantly decreased in the same group. Total release of creatine phosphokinase into perfusate was significantly less in the group G compared to the control group.

CONCLUSION

These results indicate possible direct effect of G on myocardium. Lower cardiac reperfusion injury is associated with kinase cascades following activation of GalR2 receptor by G agonist.

Keywords:

isolated perfused rat heart
ischemia
reperfusion
GalR2 receptor agonist
intracellular signaling

Authors:

Gorbunov A.S.

Cardiology Research Institute, Tomsk National Research Medical Center

Sidorova M.V.

Chazov National Medical Research Center of Cardiology

Palkeeva M.E.

Chazov National Medical Research Center of Cardiology

Molokoedov A.S.

Chazov National Medical Research Center of Cardiology

Pisarenko O.I.

Chazov National Medical Research Center of Cardiology

Received:

31.01.2024

Accepted:

17.04.2024

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