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Starikova E.A.

Institute of Experimental Medicine;
Pavlov First Saint Petersburg State Medical University;
Institute of Medical Education, Almazov National Medical Research Center

Mammedova J.T.

Institute of Experimental Medicine

Sokolov A.V.

Institute of Experimental Medicine

Podoplelova N.A.

Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology

Malashicheva A.B.

Almazov National Medical Research Center;
Cytology Institute of the Russian Academy of Sciences

Porembskaya O.Ya.

Institute of Experimental Medicine;
Mechnikov North-Western State Medical University

Zinserling V.A.

Almazov National Medical Research Center

Solovyev N.V.

Marimix LLC

Antithrombotic Effects of Blue Mussel Acid Hydrolysate

Authors:

Starikova E.A., Mammedova J.T., Sokolov A.V., Podoplelova N.A., Malashicheva A.B., Porembskaya O.Ya., Zinserling V.A., Solovyev N.V.

More about the authors

Journal: Journal of Venous Disorders. 2023;17(4): 302‑311

DOI: 10.17116/flebo202317041302

Read: 3023 times

Contact the author
Table of contents

ANTITHROMBOTIC EFFECTS OF BLUE MUSSEL ACID HYDROLYSATE
ANTITHROMBOTIC EFFECTS OF BLUE MUSSEL ACID HYDROLYSATE

To cite this article:

Starikova EA, Mammedova JT, Sokolov AV, et al. Antithrombotic Effects of Blue Mussel Acid Hydrolysate. Journal of Venous Disorders. 2023;17(4):302‑311. (In Russ., In Engl.)
https://doi.org/10.17116/flebo202317041302

Подписка 2026 Флебология (Journal of Venous Disorders)
МСФ на ЯМ
Использование инфографики авторами научных работ
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Abstract:

Thrombosis is a venous or arterial vascular that can sometimes cause fatal outcomes. Development of effective pharmacological agents for antithrombotic therapy is still an urgent scientific and practical objective.

OBJECTIVE

To study the antithrombotic effects of blue mussel (Mytilus edulis) hydrolysate preparation.

MATERIAL AND METHODS

Biochemical methods were used to analyze the effect of blue mussel hydrolysate on enzymatic activity of key serine proteases in clotting cascade. We assessed the structure of actin cytoskeleton and endothelial monolayer morphology in vitro to investigate the effect of this drug on biological activity of thrombin. Anticoagulant effect of hydrolysate was investigated in a model of posterior vena cava thrombosis in rats.

RESULTS

The drug inhibits serine proteinases of clotting cascade (FIIa, FXa, FXIa, FXIIa), in particular, enzymatic activity of thrombin (FIIa) against fibrinogen and synthetic substrates Tos-Gly-Pro-Arg-pNA and Ala-Ala-Arg-pNA. Anti-thrombin activity of undiluted drug was about 6.5 AT-U/ml. Hydrolysate inhibited the ability of thrombin to cause actin cytoskeleton remodeling and endothelial monolayer disintegration. Subcutaneous injections significantly changed thrombus structure and reduced the probability of clotting in a model of posterior vena cava thrombosis in rats.

CONCLUSION

M. edulis hydrolysate may be a basis for development of new drugs for therapy of hypercoagulable conditions.

Keywords:

M.edulis hydrolysate
venous thrombosis
antithrombotic therapy
thrombin
endothelial cells
anticoagulation

Authors:

Starikova E.A.

Institute of Experimental Medicine;
Pavlov First Saint Petersburg State Medical University;
Institute of Medical Education, Almazov National Medical Research Center

Mammedova J.T.

Institute of Experimental Medicine

Sokolov A.V.

Institute of Experimental Medicine

Podoplelova N.A.

Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology

Malashicheva A.B.

Almazov National Medical Research Center;
Cytology Institute of the Russian Academy of Sciences

Porembskaya O.Ya.

Institute of Experimental Medicine;
Mechnikov North-Western State Medical University

Zinserling V.A.

Almazov National Medical Research Center

Solovyev N.V.

Marimix LLC

Received:

03.10.2023

Accepted:

14.10.2023

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References:

  1. Porembskaya OYa, Lobastov KV, Kravchuk VN, Toropova YaG, Laberko LA, Chesnokov MSh, Bulavinova NI, Chervyak MV, Saiganov SA. Pulmonary embolism — scattered elements of incomplete puzzle. Flebologiya. 2021;15(3):188-198. (In Russ.). https://doi.org/10.17116/flebo202115031188
  2. Yang R, Liu G, Deng C. Pulmonary embolism with chronic obstructive pulmonary disease. Chronic Dis Transl Med. 2021;7(3):149-156.  https://doi.org/10.1016/j.cdtm.2021.04.001
  3. Lee PH, Fu PK. Pulmonary Embolism and Severe Asthma: Case Report and Literature Review. Medicina (Kaunas). 2019;55(10):647.  https://doi.org/10.3390/medicina55100647
  4. Lizarralde-Iragorri MA, Shet AS. Sickle Cell Disease: A Paradigm for Venous Thrombosis Pathophysiology. Int J Mol Sci. 2020;21(15):5279. PMID: 32722421; PMCID: PMC7432404. https://doi.org/10.3390/ijms21155279
  5. Kalinin RE, Suchkov IA, Filimonov VB, Mzhavanadze ND, Agapov AB, Golovchenko AS. Venous Thromboembolism in COVID-19 Patients During the First and the Second Waves of the Pandemic: Real-Practice Data. Flebologiya. 2022;16(2):122-129. (In Russ.). https://doi.org/10.17116/flebo202216021122
  6. Chapin JC, Hajjar KA. Fibrinolysis and the control of blood coagulation. Blood Rev. 2015;29(1):17-24.  https://doi.org/10.1016/j.blre.2014.09.003
  7. De Pablo-Moreno JA, Serrano LJ, Revuelta L, Sánchez MJ, Liras A. The Vascular Endothelium and Coagulation: Homeostasis, Disease, and Treatment, with a Focus on the Von Willebrand Factor and Factors VIII and V. Int J Mol Sci. 2022;23(15):8283. https://doi.org/10.3390/ijms23158283
  8. Dwivedi R, Pomin VH. Marine Antithrombotics. Mar Drugs. 2020;18(10):514.  https://doi.org/10.3390/md18100514
  9. Fernando IPS, Jayawardena TU, Wu J, Fernando IPS, Jayawardena TU, Wu J. Marine proteins and peptides: Production, biological activities, and potential applications. Food Innovation and Advances. 2023;2(2):69-84.  https://doi.org/10.48130/FIA-2023-0009
  10. Jung WK, Kim SK. Isolation and characterisation of an anticoagulant oligopeptide from blue mussel, Mytilus edulis. Food Chemistry. 2009;117(4):687-692.  https://doi.org/10.1016/j.foodchem.2009.04.077
  11. Feng L, Tu M, Qiao M, Fan F, Chen H, Song W, Du M. Thrombin inhibitory peptides derived from Mytilus edulis proteins: identification, molecular docking and in silico prediction of toxicity. Eur Food Res Technol. 2018;244(2):207-217.  https://doi.org/10.1007/s00217-017-2946-7
  12. Qiao M, Tu M, Wang Z, Mao F, Chen H, Qin L, Du M. Identification and Antithrombotic Activity of Peptides from Blue Mussel (Mytilus edulis) Protein. Int J Mol Sci. 2018;19(1):138.  https://doi.org/10.3390/ijms19010138
  13. Qiao M, Tu M, Chen H, Mao F, Yu C, Du M. Identification and In Silico Prediction of Anticoagulant Peptides from the Enzymatic Hydrolysates of Mytilus edulis Proteins. Int J Mol Sci. 2018;19(7):2100. https://doi.org/10.3390/ijms19072100
  14. Starikova E, Mammedova J, Ozhiganova A, Lebedeva A, Malashicheva A, Semenova D, Khokhlova E, Mameli E, Caporali A, Wills J, Sokolov A. Protective Role of Mytilus edulis Hydrolysate in Lipopolysaccharide-Galactosamine Acute Liver Injury. Front Pharmacol. 2021;12:667572. https://doi.org/10.3389/fphar.2021.667572
  15. Kotova YN, Podoplelova NA, Obydennyy SI, Kostanova EA, Ryabykh AA, Demyanova AS, Biriukova MI, Rosenfeld MA, Sokolov AV, Chambost H, Kumskova MA, Ataullakhanov FI, Alessi M-C, Panteleev MA. Binding of Coagulation Factor XIII Zymogen to Activated Platelet Subpopulations: Roles of Integrin αIIbβ3 and Fibrinogen. Thromb Haemost. 2019;119(6): 906-915.  https://doi.org/10.1055/s-0039-1683912
  16. Yang WG, Wang Z, Xu SY. A new method for determination of antithrombotic activity of egg white protein hydrolysate by microplate reader. Chinese Chemical Letters. 2007;18(4):449-451.  https://doi.org/10.1016/j.cclet.2007.02.014
  17. Krupyanko IV. Correction of equations for calculating constants of two-parameter types of inhibition and activation of enzymes. Biochemistry. 2007; 72(4):473-485. (In Russ.).
  18. Baudin B, Bruneel A, Bosselut N, Vaubourdolle M. A protocol for isolation and culture of human umbilical vein endothelial cells. Nat Protoc. 2007;2(3):481-485.  https://doi.org/10.1038/nprot.2007.54
  19. Varma MR, Varga AJ, Knipp BS, Sukheepod P, Upchurch GR, Kunkel SL, Wakefield TW, Henke PK. Neutropenia impairs venous thrombosis resolution in the rat. J Vasc Surg. 2003;38(5):1090-1098. https://doi.org/10.1016/s0741-5214(03)00431-2
  20. Melnichnikova OS, Semenov AP, Panov AV, Sirotkina OV, Nazarova IA, Karpenko AM, Vavilova TV. Investigation of thrombin generation in patients with stable ischemic disease with previous myocardial infarction. Translational Medicine. 2019;6(2):37-45. (In Russ.). https://doi.org/10.18705/2311-4495-2019-6-2-37-45
  21. Giri H, Srivastava AK, Naik UP. Apoptosis signal-regulating kinase-1 regulates thrombin-induced endothelial permeability. Vascul Pharmacol. 2022; 145:107088. https://doi.org/10.1016/j.vph.2022.107088
  22. Khismatullin RR, Shakirova AZ, Weisel JW, Litvinov RI. Age-Dependent Differential Staining of Fibrin in Blood Clots and Thrombi. BioNanoSci. 2020;10(1):370-374.  https://doi.org/10.1007/s12668-019-00701-4
  23. Litvinov RI, Weisel JW. Blood clot contraction: Mechanisms, pathophysiology, and disease. Res Pract Thromb Haemost. 2023;7(1):100023. PMID: 36760777; PMCID: PMC9903854. https://doi.org/10.1016/j.rpth.2022.100023
  24. Gillespie AH, Doctor A. Red Blood Cell Contribution to Hemostasis. Front Pediatr. 2021;9:629824. https://doi.org/10.3389/fped.2021.629824
  25. Popović M, Smiljanić K, Dobutović B, Syrovets T, Simmet T, Isenović ER. Thrombin and vascular inflammation. Mol Cell Biochem. 2012;359(1):301-313.  https://doi.org/10.1007/s11010-011-1024-x
  26. Andrikopoulos P, Kieswich J, Harwood SM, Baba A, Matsuda T, Barbeau O, Jones K, Eccles SA, Yaqoob MM. Endothelial Angiogenesis and Barrier Function in Response to Thrombin Require Ca2+ Influx through the Na+/Ca2+ Exchanger. Journal of Biological Chemistry. 2015;290(30):18412-18428. PMID: 25979335; PMCID: PMC4513102. https://doi.org/10.1074/jbc.M114.628156
  27. Wang B, Li L, Chi CF, Ma JH, Luo HY, Xu Y. Purification and characterisation of a novel antioxidant peptide derived from blue mussel (Mytilus edulis) protein hydrolysate. Food Chem. 2013;138(2-3):1713-1719. https://doi.org/10.1016/j.foodchem.2012.12.002
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