Neutrophil extracellular traps in a patient with ulcerative colitis

Novikov D.G.; Zolotov A.N.; Bikbavova G.R.; Livzan M.A.; Telyatnikova L.I.

doi:https://doi.org/10.17116/dokgastro20221102131

Novikov D.G.

Omsk State Medical University

Zolotov A.N.

Omsk State Medical University

Bikbavova G.R.

Omsk State Medical University

Livzan M.A.

Omsk State Medical University

ORCID: 0000-0002-6581-7017

Telyatnikova L.I.

Clinical and Diagnostic Center Ultramed

Neutrophil extracellular traps in a patient with ulcerative colitis

Authors:

Novikov D.G., Zolotov A.N., Bikbavova G.R., Livzan M.A., Telyatnikova L.I.

More about the authors

Journal: Russian Journal of Evidence-Based Gastroenterology. 2022;11(2): 31‑38

DOI: 10.17116/dokgastro20221102131

Read: 2214 times

Download PDF (RU)

Contact the author

Table of contents

To cite this article:

Novikov DG, Zolotov AN, Bikbavova GR, Livzan MA, Telyatnikova LI. Neutrophil extracellular traps in a patient with ulcerative colitis. Russian Journal of Evidence-Based Gastroenterology. 2022;11(2):31‑38. (In Russ.)
https://doi.org/10.17116/dokgastro20221102131

Подписка 2026 Доказательная гастроэнтерология (Russian Journal of Evidence-Based Gastroenterology)

Использование инфографики авторами научных работ

Close metadata

OBJECTIVE

To evaluate neutrophils extracellular traps (NETs) formation in a patient with ulcerative colitis (UC).

MATERIAL AND METHODS

NETs formation was studied on neutrophils isolated from the whole blood of a UC patient on a ficol-verografin gradient. We compared NETs formation in UC patient and healthy volunteers (controls) in intact neutrophils and after 30 minutes of probiotics stimulation.

RESULTS

Neutrophils in a patient with UC demonstrated a more pronounced ability to NETs formation both intact and after stimulation. NETs formed in a UC patient after probiotics stimulation had significant network size, exceeding the neutrophil size by 10—18 times, while NETs in controls were only 4—8 times larger. The NETs of UC patients demonstrated a more pronounced ability to capture bacilliform bacteria, with 21 bacilli captured, compared to controls Me 7.5 (LQ 4.75; HQ 10.5). Except for evaluating the NETs formation, UC activity in a patient was assessed by colonoscopy, histology, complete blood count, C-reactive protein, and fecal calprotectin.

CONCLUSION

NETs formation in UC patient was more pronounced than in healthy controls. Proinflammatory proteins, cytokines, and other inflammatory mediators in the blood of UC patients may change the composition and density of receptors on the neutrophil membrane and trigger receptor-mediated NETosis.

Keywords:

ulcerative colitis

NETosis

neutrophil extracellular traps

neutrophils

Authors:

Novikov D.G.

Omsk State Medical University

Zolotov A.N.

Omsk State Medical University

Bikbavova G.R.

Omsk State Medical University

Livzan M.A.

Omsk State Medical University

ORCID: 0000-0002-6581-7017

Telyatnikova L.I.

Clinical and Diagnostic Center Ultramed

Received:

03.03.2022

Accepted:

22.03.2022

Close metadata

References:

Ramos G, Papadakis K. Mechanisms of Disease: Inflammatory Bowel Diseases. Mayo Clinic Proceedings. 2019;94(1):155-165. https://doi.org/10.1016/j.mayocp.2018.09.013
GBD 2017 Inflammatory Bowel Disease Collaborators. The global, regional, and national burden of inflammatory bowel disease in 195 countries and territories, 1990—2017: a systematic analysis for the Global Burden of Disease Study 2017. Lancet. Gastroenterology and Hepatology. 2020;5(1):17-30. https://doi.org/10.1016/S2468-1253(19)30333-4
Nikitin AV, Khavkin AI, Skvortsova TA, Volynets GV, Atameeva AO. Combination of ulcerative colitis with cirrhosis of the liver in the outcome of primary sclerosing cholangitis. Eksperimental’naya i klinicheskaya gastroenterologiya. 2020;174(5):104-107. (In Russ.). https://doi.org/10.31146/1682-8658-ecg-177-5-104-107
Bikbavova GR, Livzan MA, Sovalkin VI, Turchaninov DV, Lopatina OE, Tretyakova TV, Gordienko TS, Ahramovich AP. Modern lifestyle and its impact on the ulcerative colitis incidence. Arhiv vnutrennej mediciny. 2019;9(3):188-194. (In Russ.). https://doi.org/10.20514/2226-6704-2019-9-3-188-193
Belousova EA, Abdulganieva DI, Alekseeva OP, Alekseenko SA, Baranovskij AYu, Valujskih EYu, Golovenko AO, Golovenko OV, Zhigalova TN, Knyazev OV, Kulyapin AV, Lahin AV, Livzan MA, Lubyanskaya TG, Nikolaeva NN, Nikitina NV, Nikulina IV, Osipenko MF, Pavlenko VV, Parfenov AI, Rogachikov YE, Svetlova IO, Tkachev AV, Tkachenko EI, Khalif IL, Chashkova EY, Shchukina OB, Yazenok NS, Yakovlev AA. Social and demographic characteristics, features of disease course and treatment options of inflammatory bowel disease in Russia: results of two multicenter studies. Al’manah klinicheskoj mediciny. 2018;46(5):445-463. (In Russ.). https://doi.org/10.18786/2072-0505-2018-46-5-445-463
Kaistha A, Levine J. Inflammatory bowel disease: the classic gastrointestinal autoimmune disease. Current Problems in Pediatric and Adolescent Health Care. 2014;44(11):328-334. https://doi.org/10.1016/j.cppeds.2014.10.003
Emelyanova VA, Demidov AA, Kostenko NV, Chernysheva EN. Inflammatory bowel disease: cytochemical activity of intracellular enzymes of peripheral blood neutrophils and monocytes in diagnosis. Kubanskij nauchnyj medicinskij vestnik. 2017;24(3):137-141. (In Russ.). https://doi.org/10.25207/1608-6228-2017-24-3-137-141
De Bont CM, Boelens WC, Pruijn GJM. NETosis, complement, and coagulation: a triangular relationship. Cellular and Molecular Immunology. 2019;16(1):19-27. https://doi.org/10.1038/s41423-018-0024-0
Ivashkin VT, Shelygin YuA, Halif IL, Belousova EA, Shifrin OS, Abdulganieva DI, Abdulhakov RA, Alekseeva OP, Alekseenko SA, Achkasov SI, Baranovskij AYu, Bolihov KV, Valujskih EYu, Vardanyan AV, Veselov AV, Veselov VV, Golovenko AO, Golovenko OV, Grigor’ev EG, Gubonina IV, Zhigalova TN, Kashnikov VN, Kizova EA, Knyazev OV, Kostenko NV, Kulyapin AV, Morozova NA, Murav’ev AV, Nizov AA, Nikitina NV, Nikolaeva NN, Nikulina NV, Odincova AH, Osipenko MF, Pavlenko VV, Parfenov AI, Poluektova EA, Potapov AS, Rumyancev VG, Svetlova IO, Sitkin SI, Timerbulatov VM, Tkachev AV, Tkachenko EI, Frolov SA, Hubezov DA, Chashkova EYu, Shapina MV, Shchukina OB, Yakovlev AA. Clinical guide of Russian Association of Gastroenterology and Russian Association of Coloproctology on diagnostics and treatment of ulcerative colitis. Koloproktologia. 2017;59(1):6-30. (In Russ.).
Abd El Hafez A, Mohamed AS, Shehta A, Sheta HAEAS. Neutrophil extracellular traps-associated protein peptidyl arginine deaminase 4 immunohistochemical expression in ulcerative colitis and its association with the prognostic predictors. Pathology, Research and Practice. 2020;216(10):153102. https://doi.org/10.1016/j.prp.2020.153102
Bennike TB, Carlsen TG, Ellingsen T, Bonderup OK, Glerup H, Bøgsted M, Christiansen G, Birkelund S, Stensballe A, Andersen V. Neutrophil Extracellular Traps in Ulcerative Colitis: A Proteome Analysis of Intestinal Biopsies. Inflammatory Bowel Diseases. 2015;21(9):2052-2067. https://doi.org/10.1097/MIB.0000000000000460
Dinallo V, Marafini I, Di Fusco D, Laudisi F, Franzè E, Di Grazia A, Figliuzzi MM, Caprioli F, Stolfi C, Monteleone I, Monteleone G. Neutrophil Extracellular Traps Sustain Inflammatory Signals in Ulcerative Colitis. Journal of Crohn’s and Colitis. 2019;13(6): 772-784. https://doi.org/10.1093/ecco-jcc/jjy215
Turner D, Ricciuto A, Lewis A, D’Amico F, Dhaliwal J, Griffiths AM, Bettenworth D, Sandborn WJ, Sands BE, Reinisch W, Schölmerich J, Bemelman W, Danese S, Mary JY, Rubin D, Colombel JF, Peyrin-Biroulet L, Dotan I, Abreu MT, Dignass A; International Organization for the Study of IBD. STRIDE-II: An Update on the Selecting Therapeutic Targets in Inflammatory Bowel Disease (STRIDE) Initiative of the International Organization for the Study of IBD (IOIBD): Determining Therapeutic Goals for Treat-to-Target strategies in IBD. Gastroenterology. 2021;160(5):1570-1583. https://doi.org/10.1053/j.gastro.2020.12.031