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Pronin I.N.

Burdenko Neurosurgical Center;
Russian Medical Academy of Continuing Professional Education

Postnov A.A.

Burdenko Neurosurgical Center;
The Lebedev Physical Institute of the Russian Academy of Sciences

Lipengolts A.A.

Blokhin National Medical Research Center of Oncology

Pavlova G.V.

Burdenko Neurosurgical Center;
Institute of Higher Nervous Activity and Neurophysiology of the Russian Academy of Sciences

Revishchin A.V.

Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences

Skribitsky V.A.

Blokhin National Medical Research Center of Oncology

Finogenova Yu.A.

Blokhin National Medical Research Center of Oncology

Smirnova A.V.

Blokhin National Medical Research Center of Oncology

Shpakova K.E.

Blokhin National Medical Research Center of Oncology

Grigorieva E.Yu.

Blokhin National Medical Research Center of Oncology

Kozlova Yu.A.

Russian Medical Academy of Continuous Professional Education;
Botkin Hospital

Alekseeva A.I.

Burdenko Neurosurgical Center;
Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences;
Avtsyn Research Institute of Human Morphology of Federal state budgetary scientific institution «Petrovsky National Research Centre of Surgery»

A Novel Rat Glioblastoma 101/8 Model: A Comparative PET-CT Study with C6 Rat model

Authors:

Pronin I.N., Postnov A.A., Lipengolts A.A., Pavlova G.V., Revishchin A.V., Skribitsky V.A., Finogenova Yu.A., Smirnova A.V., Shpakova K.E., Grigorieva E.Yu., Kozlova Yu.A., Alekseeva A.I.

More about the authors

Journal: Burdenko's Journal of Neurosurgery. 2024;88(6): 54‑62

DOI: 10.17116/neiro20248806154

Read: 1714 times

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Table of contents

A NOVEL RAT GLIOBLASTOMA 101/8 MODEL: A COMPARATIVE PET-CT STUDY WITH C6 RAT MODEL
A NOVEL RAT GLIOBLASTOMA 101/8 MODEL: A COMPARATIVE PET-CT STUDY WITH C6 RAT MODEL

To cite this article:

Pronin IN, Postnov AA, Lipengolts AA, et al. . A Novel Rat Glioblastoma 101/8 Model: A Comparative PET-CT Study with C6 Rat model. Burdenko's Journal of Neurosurgery. 2024;88(6):54‑62. (In Russ., In Engl.)
https://doi.org/10.17116/neiro20248806154

Подписка 2025 Журнал «Вопросы нейрохирургии» имени Н.Н. Бурденко (Burdenko's Journal of Neurosurgery)
МСФ на ЯМ
Использование инфографики авторами научных работ
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Abstract:

The development of new drugs in nuclear medicine for diagnosis or treatment (chemotherapy) of brain tumors, in particular gliomas, is inextricably linked with the use of tumor models in animals (usually rats).

OBJECTIVE

To compare the widely used glioma cell model C6 and the new experimental tissue model of glioblastoma 101.8.

MATERIAL AND METHODS

A comparison was made of the diagnostic and morphological characteristics of the presented glioma models in two groups of animals with intracranially implanted tissue strain of experimental glioblastoma 101.8 (n=4) and the C6 glioma cell line (n=4) throughout the tumor development cycle within the rat brain. To monitor the progress of tumor growth and development, each animal underwent repeated diagnostic studies using PET-CT with 18F-FDG and 18F-FET to assess the metabolic activity and volume of the tumor. Also MRI images were collected. After the end of data acquisition, a histological examination of the tumor was carried out.

RESULTS

The tissue model of glioblastoma 101.8 demonstrated rapid growth and pronounced accumulation of the tracers in all animals during the tumor observation cycle. Formation of intratumoral necrosis and signs of disruption of the blood-brain barrier (BBB) were detected. In PET-CT studies of animals with a transplanted C6 tumor, no visible necrosis in the tumor structure was observed. Tumor growth was less rapid than in the case of model 101.8. The obtained morphological characteristics of 101.8 tumors transplanted into the rat brain demonstrated similar properties observed in real clinical conditions in patients with glioblastoma of the brain (necrosis, neovascularization, multiple pseudopalisade structures).

CONCLUSIONS

Tumor model 101.8 can be recommended for scientific research as it most closely reproduces the diagnostic and morphological features of a human glioblastoma.

Keywords:

glioma model 101.8
glioma model C6
micro-PET
glioblastoma
micro-CT with contrast agent
animal MRI

Authors:

Pronin I.N.

Burdenko Neurosurgical Center;
Russian Medical Academy of Continuing Professional Education

Postnov A.A.

Burdenko Neurosurgical Center;
The Lebedev Physical Institute of the Russian Academy of Sciences

Lipengolts A.A.

Blokhin National Medical Research Center of Oncology

Pavlova G.V.

Burdenko Neurosurgical Center;
Institute of Higher Nervous Activity and Neurophysiology of the Russian Academy of Sciences

Revishchin A.V.

Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences

Skribitsky V.A.

Blokhin National Medical Research Center of Oncology

Finogenova Yu.A.

Blokhin National Medical Research Center of Oncology

Smirnova A.V.

Blokhin National Medical Research Center of Oncology

Shpakova K.E.

Blokhin National Medical Research Center of Oncology

Grigorieva E.Yu.

Blokhin National Medical Research Center of Oncology

Kozlova Yu.A.

Russian Medical Academy of Continuous Professional Education;
Botkin Hospital

Alekseeva A.I.

Burdenko Neurosurgical Center;
Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences;
Avtsyn Research Institute of Human Morphology of Federal state budgetary scientific institution «Petrovsky National Research Centre of Surgery»

Received:

15.09.2024

Accepted:

20.09.2024

Close metadata

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