Objective. To investigate parameters of insulin resistance and arterial stiffness in different stages of cardiovascular autonomic neuropathy in patients with diabetes mellitus type 2 (DM2). Material and methods. The study involved 65 patients with DM: 12 - without verified cardiovascular disease (CVD) and cardiovascular autonomic neuropathy (CAN), 14 - with subclinical, 18 - functional, 21 - functional-organic stage of CAN. A set of methods for assessment of cardiovascular system and main properties of arteries was implemented. Results and conclusions. The development of CAN in patients with type 2 DM is accompanied with the increased level of immunoreactive insulin, insulin-resistance index. A trend towards increasing vascular wall stiffness in DM patients without CVD and CAN. The subclinical stage of CAN was characterized by increased pulse wave velocity in the aorta (PWV), aortic and brachial (AIXbr) augmentation indexes throughout the day, active and passive periods of day; functional - increased AIXbr during the night, PWV throughout the day, active and passive periods of day compared to subclinical stage; functional-organic - by increased and higher pathological values of vascular wall stiffness. Daily values of PWV and ambulatory arterial stiffness index in patients with functional-organic CAN were statistically significantly higher compared to groups with subclinical and functional stages of CAN.