The site of the Media Sphera Publishers contains materials intended solely for healthcare professionals.
By closing this message, you confirm that you are a certified medical professional or a student of a medical educational institution.

Login
EN
+7 (495) 482-4118
+7 (495) 482-0604
+8 800 250-18-12
  • (toll-free number for subscriptions)
    Mon-Fri from 10 a.m. to 6 p.m.

  • © 1998-2025
+7 (495) 482-43-29
EN
All journals
Journal
Year
Year
Search result: 0

Brovkina A.F.

Russian Medical Academy of Continuous Professional Education;
Botkin Hospital

Kabardikova L.A.

Russian Medical Academy of Continuous Professional Education;
Moscow Multidisciplinary Scientific and Clinical Center named after S.P. Botkin

Shcherbakov P.A.

Moscow Multidisciplinary Scientific and Clinical Center named after S.P. Botkin

Bure I.V.

Russian Medical Academy of Continuous Professional Education

Mirzaev K.B.

Russian Medical Academy of Continuous Postgraduate Education

Uveal melanoma and its local inflammation — good or bad for the patient?

Authors:

Brovkina A.F., Kabardikova L.A., Shcherbakov P.A., Bure I.V., Mirzaev K.B.

More about the authors

Journal: Russian Annals of Ophthalmology. 2025;141(1): 5‑12

DOI: 10.17116/oftalma20251410115

Read: 1125 times

Buy for 300₽
Contact the author
Table of contents

UVEAL MELANOMA AND ITS LOCAL INFLAMMATION — GOOD OR BAD FOR THE PATIENT?
UVEAL MELANOMA AND ITS LOCAL INFLAMMATION — GOOD OR BAD FOR THE PATIENT?

To cite this article:

Brovkina AF, Kabardikova LA, Shcherbakov PA, Bure IV, Mirzaev KB. Uveal melanoma and its local inflammation — good or bad for the patient? Russian Annals of Ophthalmology. 2025;141(1):5‑12. (In Russ.)
https://doi.org/10.17116/oftalma20251410115

Подписка 2026 Вестник офтальмологии (Russian Annals of Ophthalmology)
 
МСФ на ЯМ
Использование инфографики авторами научных работ
Recommended articles:
Glial fibrillary acidic protein: is there a rela­tionship with the course of multiple scle­rosis and traumatic brain injury before the onset of multiple scle­rosis?. S.S. Korsakov Journal of Neurology and Psychiatry. 2024;(10):80-84
The role of some markers of the infe­ctious process in the early diagnosis and evaluation of the effe­ctiveness of anti­biotic therapy in obstetric sepsis. Russian Bulletin of Obstetrician-Gynecologist. 2025;(2):15-20
Triglycerides-Glucose Index as an inte­gral biomarker of insu­lin resi­stance and cardiovascular diseases. Russian Journal of Preventive Medi­cine. 2025;(5):124-129
Myokines — the cardiometabolic risk pote­ntial biomarkers. Russian Journal of Preventive Medi­cine. 2025;(7):119-126
Abstract:

The 21st century is characterized by the study of the features of malignant tumor infiltration by cellular components of the immune system. While some findings suggest a favorable role of lymphoid infiltration in tumors and its positive impact on survival due to immune defense, the opposite is observed in uveal melanoma (UM): lymphoid infiltration significantly worsens the patient’s prognosis. Research on this topic is limited, with some studies confined to experimental models.

PURPOSE

This study investigated the correlation between microRNA-155 expression in the plasma of UM patients after primary enucleation and with pathomorphological inflammatory changes.

MATERIAL AND METHODS

Eighty-three patients with UM were examined, in 21 cases lymphoid infiltration of the primary tumor was detected, in 6 cases accompanied by a microenvironment. The degree of tumor infiltration with lymphocytes was estimated as a percentage of the total number of cells in the analyzed field, ranging from 1% to 25%. Real-time polymerase chain reaction was used to determine microRNA-155 expression in the plasma of all patients before and after enucleation, and its correlation with the level of lymphoid infiltration was analyzed. Postoperative follow-up lasted 6 to 53 months (mean 21.5±14.9 months). In two cases, in the presence of a tumor microenvironment, metastases to the liver were detected 6 and 10 months after enucleation. MicroRNA-155 levels in a volunteer group were used as the control baseline, set at 100% for clarity and convenience.

RESULTS

MicroRNA-155 expression levels were higher in patients with lymphoid infiltration (p<0.05) and tended to increase with the degree of primary tumor infiltration (p<0.001), which in itself, with the appearance of a perifocal lymphoid environment, indicated a poor prognosis.

CONCLUSION

Monitoring microRNA-155 expression levels in the plasma of UM patients may help predict occult metastasis prior to enucleation. This is not only crucial for clinical follow-up but may also play a significant role in the development of targeted therapies.

Keywords:

uveal melanoma
microRNA-155
biomarker
lymphoid infiltration

Authors:

Brovkina A.F.

Russian Medical Academy of Continuous Professional Education;
Botkin Hospital

Kabardikova L.A.

Russian Medical Academy of Continuous Professional Education;
Moscow Multidisciplinary Scientific and Clinical Center named after S.P. Botkin

Shcherbakov P.A.

Moscow Multidisciplinary Scientific and Clinical Center named after S.P. Botkin

Bure I.V.

Russian Medical Academy of Continuous Professional Education

Mirzaev K.B.

Russian Medical Academy of Continuous Postgraduate Education

Received:

16.09.2024

Accepted:

06.10.2024

Close metadata

References:

  1. Cavallo F, De Giovanni C, Nanni P, Forni G, Lollini PL. 2011: the immune hallmarks of cancer. Cancer Immunol Immunother. 2011;60(3):319-326.  https://doi.org/10.1007/s00262-010-0968-0
  2. O’Connell RM, Kahn D, Gibson WSJ, Round JL, Scholz RL, Chaudhuri AA, Kahn ME, Rao DS, Baltimore D. MicroRNA-155 Promotes Autoimmune Inflammation by Enhancing Inflammatory T Cell Development. Immunity. 2010;33(4):607-619.  https://doi.org/10.1016/j.immuni.2010.09.009
  3. Vigorito E, Kohlhaas S, Lu D, Leyland R. miR-155: an ancient regulator of the immune system. Immunol Rev. 2013;253(1):146-157.  https://doi.org/10.1111/imr.12057
  4. Morozov VI. Hematoophthalmic barrier: structural and functional features. Rossijskij oftal’mologicheskij zhurnal. 2017;10(4):68-72 (In Russ.). https://doi.org/10.21516/2072-0076-2017-10-4-68-72
  5. Hodge J, Wang F, Wang J, Liu Q, Saaoud F, Wang Y, Singh UP, Chen H, Luo M, Ai W, Fan D. Overexpression of microRNA-155 enhances the efficacy of dendritic cell vaccine against breast cancer. Oncoimmunology. 2020; 9(1):e1724761. https://doi.org/10.1080/2162402x.2020.1724761
  6. Monnot GC, Martinez-Usatorre A, Lanitis E, Lopes SF, Cheng WC, Ho PC, Irving M, Coukos G, Donda A, Romero P. miR-155 Overexpression in OT-1 CD8+ T Cells Improves Anti-Tumor Activity against Low-Affinity Tumor Antigen. Mol Ther Oncolytics. 2020;16:111-123.  https://doi.org/10.1016/j.omto.2019.12.008
  7. Croci DO, Zacarías Fluck MF, Rico MJ, Matar P, Rabinovich GA, Scharovsky OG. Dynamic cross-talk between tumor and immune cells in orchestrating the immunosuppressive network at the tumor microenvironment. Cancer Immunol Immunother. 2007;56(11):1687-1700. https://doi.org/10.1007/s00262-007-0343-y
  8. Durante MA, Rodriguez DA, Kurtenbach S, Kuznetsov JN, Sanchez MI, Decatur CL, Snyder H, Feun LG, Livingstone AS, Harbour JW. Single-cell analysis reveals new evolutionary complexity in uveal melanoma. Nat Commun. 2020;11(1):496.  https://doi.org/10.1038/s41467-019-14256-1
  9. Kujala E, Mäkitie T, Kivelä T. Very Long-Term Prognosis of Patients with Malignant Uveal Melanoma. Invest Ophthalmol Vis Sci. 2003;44(11):4651-4659. https://doi.org/10.1167/iovs.03-0538
  10. Bronkhorst IH, Jager MJ. Uveal Melanoma: The Inflammatory Microenvironment. J Innate Immun. 2012;4(5-6):454-462.  https://doi.org/10.1159/000334576
  11. Saakyan SV, Zakharova GP, Myakoshina EB. Cellular microenvironment of uveal melanoma: clinical and morphological correlations and predictors of unfavorable prognosis. Molekulyarnaya medicina. 2020;18(3):27-33 (In Russ.). https://doi.org/10.29296/24999490-2020-03-04
  12. Shamanova AYu, Kazachkov EL, Panova IE, Vazhenin AV, Shamaeva TN, Alymov EA. Features of the lymphocytic microenvironment in metastatic uveal melanoma. Ural’skij medicinskij zhurnal. 2021;20(2):36-42 (In Russ.). https://doi.org/10.52420/2071-5943-2021-20-2-36-42
  13. Gasparini P, Lovat F, Fassan M, Casadei L, Cascione L, Jacob NK, Carasi S, Palmieri D, Costinean S, Shapiro CL, Huebner K, Croce CM. Protective role of miR-155 in breast cancer through RAD51 targeting impairs homologous recombination after irradiation. Proc Natl Acad Sci U S A. 2014;111(12):4536-4541. https://doi.org/10.1073/pnas.1402604111
  14. Kong W, He L, Richards EJ, Challa S, Xu CX, Permuth-Wey J, Lancaster JM, Coppola D, Sellers TA, Djeu JY, Cheng JQ. Upregulation of miRNA-155 promotes tumour angiogenesis by targeting VHL and is associated with poor prognosis and triple-negative breast cancer. Oncogene. 2014;33(6):679-689.  https://doi.org/10.1038/onc.2012.636
  15. Van Roosbroeck K, Fanini F, Setoyama T, Ivan C, Rodriguez-Aguayo C, Fuentes-Mattei E, Xiao L, Vannini I, Redis RS, D’Abundo L, Zhang X, Nicoloso MS, Rossi S, Gonzalez-Villasana V, Rupaimoole R, Ferracin M, Morabito F, Neri A, Ruvolo PP, Calin GA. Combining Anti-Mir-155 with Chemotherapy for the Treatment of Lung Cancers. Clin Cancer Res. 2017; 23(11):2891-2904. https://doi.org/10.1158/1078-0432.ccr-16-1025
  16. Tili E, Croce CM, Michaille JJ. miR-155: On the Crosstalk Between Inflammation and Cancer. Int Rev Immunol. 2009;28(5):264-284.  https://doi.org/10.1080/08830180903093796
  17. Zhang J, Cheng C, Yuan X, He JT, Pan QH, Sun FY. microRNA-155 acts as an oncogene by targeting the tumor protein 53-induced nuclear protein 1 in esophageal squamous cell carcinoma. Int J Clin Exp Pathol. 2014;7(2): 602-610.  https://doi.org/10.1016/j.ijcep.2014.01.015
  18. Mahesh G, Biswas R. MicroRNA-155: A Master Regulator of Inflammation. J Interferon Cytokine Res. 2019;39(6):321-330.  https://doi.org/10.1089/jir.2018.0155
  19. Brovkina AF, Yarovaya GA, Tsybikova ND. MicroRNA in Ophthalmology. Ophthalmology in Russia. 2021;18(2):188-197 (In Russ.). https://doi.org/10.18008/1816-5095-2021-2-188-197
  20. Wang J, Wang Q, Guan Y, et al. Breast cancer cell–derived microRNA-155 suppresses tumor progression via enhancing immune cell recruitment and antitumor function. J Clin Invest. 2022;132(19):e157248. https://doi.org/10.1172/jci157248
  21. Guo J, Jiang W, Xu X, Zheng X. Serum microRNA-155 in early diagnosis and prognosis of breast cancer. Int J Clin Exp Med. 2016;9(6):10289-10295. https://doi.org/10.18203/ijcem.2016.06.10289
  22. Shooshtarian B, Mohammadi-Asl J, Kohan L. The diagnostic value of miR-155 expression in the serum of patients with breast cancer according to molecular subtypes of breast cancer. Jentashapir J Health Res. 2017;8(2). https://doi.org/10.5812/jjhr.59937
  23. Hosseini Mojahed F, Ghasemi A, Rahimi M, Shafiee M, Azizi E, Mohammadi M. Clinical Evaluation of the Diagnostic Role of MicroRNA-155 in Breast Cancer. Int J Genomics. 2020;2020:9514831. https://doi.org/10.1155/2020/9514831
  24. Li Z, Yu X, Shen J, Jiang Y. MicroRNA dysregulation in uveal melanoma: A new player enters the game. Oncotarget. 2015;6(7):4562-4568. https://doi.org/10.18632/oncotarget.2923
  25. Peng J, Liu H, Liu C. MiR-155 Promotes Uveal Melanoma Cell Proliferation and Invasion by Regulating NDFIP1 Expression. Technol Cancer Res Treat. 2017;16(6):1160-1167. https://doi.org/10.1177/1533034617737923
  26. Aughton K, Kalirai H, Coupland SE. MicroRNAs and Uveal Melanoma: Understanding the Diverse Role of These Small Molecular Regulators. Int J Mol Sci. 2020;21(16):5648. https://doi.org/10.3390/ijms21165648
  27. Kozomara A, Birgaoanu M, Griffiths-Jones S. miRBase: from microRNA sequences to function. Nucleic Acids Res. 2019;47(1):155-162.  https://doi.org/10.1093/nar/gky1141
  28. Rodríguez MFB, Fernandez MB, Baameiro LN, et al. Blood Biomarkers of Uveal Melanoma: Current Perspectives. Clin Ophthalmol. 2020;14:157-169.  https://doi.org/10.2147/OPTH.S199064
  29. Brovkina AF, Tsybikova ND. MicroRNA as a Biomarker of Choroidal Melanoma Aggressiveness. Rossiyskiy oftalmologicheskiy zhurnal. 2022;15(1):7-12 (In Russ.). https://doi.org/10.21516/2072-0076-2022-15-1-7-12
  30. He M, Xu Z, Ding T, Kuang DM, Zheng L. MicroRNA-155 regulates inflammatory cytokine production in tumor-associated macrophages via targeting C/EBPbeta. Cell Mol Immunol. 2009;6(5):343-352.  https://doi.org/10.1038/cmi.2009.45
  31. Azimi F, Scolyer RA, Rumcheva P, Moncrieff M, Murali R, McCarthy SW, Saw RP, Thompson JF. Tumor-Infiltrating Lymphocyte Grade Is an Independent Predictor of Sentinel Lymph Node Status and Survival in Patients With Cutaneous Melanoma. J Clin Oncol. 2012;30(21):2678-2683. https://doi.org/10.1200/JCO.2011.37.8539
  32. Narasimhaiah D, Legrand C, Damotte D, Remark R, Munda M, De Potter P, Coulie PG. DNA alteration-based classification of uveal melanoma gives better prognostic stratification than immune infiltration, which has a neutral effect in high-risk group. Cancer Med. 2019;8(5):2122-2131. https://doi.org/10.1002/cam4.2122
  33. Durie FH, Campbell AM, Lee WR, Damato BE. Analysis of lymphocytic infiltration in uveal melanoma. Investigative Ophthalmology & Visual Science. 1990; 31(10): 2106—2110.
  34. Lagouros E, Salomao D, Thorland E, Hodge DO, Vile R, Pulido JS. Infiltrative T regulatory cells in enucleated uveal melanomas. Trans Am Ophthalmol Soc. 2009;107:223-228.  https://doi.org/10.5555/20126498
  35. Mougiakakos D, Johansson CC, Trocme E, All-Ericsson C, Economou MA, Larsson O, Seregard S, Kiessling R. Intratumoral forkhead box P3-positive regulatory T cells predict poor survival in cyclooxygenase-2-positive uveal melanoma. Cancer. 2010;116(9):2224-2233. https://doi.org/10.1002/cncr.24999
  36. Eremeeva ER, Setdikova GR, Grishina EE, Kim ID, Shikina VE. Analysis of Lymphoid Infiltration in Uveal Melanoma. Zlokachestvennye opukholi. 2022;12(3S1):188 (In Russ.).
  37. Wu R, Forget MA, Chacon J, Bernatchez C, Haymaker C, Chen JQ, Hwu P, Radvanyi LG. Adoptive T-cell Therapy Using Autologous Tumor-infiltrating Lymphocytes for Metastatic Melanoma: Current Status and Future. Cancer J. 2012;18(2):160-175.  https://doi.org/10.1097/PPO.0b013e318247bcec
  38. Krishna Y, McCarthy C, Kalirai H, Coupland SE. Inflammatory cell infiltrates in advanced metastatic uveal melanoma. Hum Pathol. 2017;66:159-166.  https://doi.org/10.1016/j.humpath.2017.06.005
  39. Javed A, Milhem M. Role of Natural Killer Cells in Uveal Melanoma. Cancers (Basel). 2020;12(12):3694. https://doi.org/10.3390/cancers12123694
  40. Gencia I, Baderca F, Avram S, Gogulescu A, Marcu A, Seclaman E, Marian C, Solovan C. A preliminary study of microRNA expression in different types of primary melanoma. Bosn J Basic Med Sci. 2020;20(2):197-208.  https://doi.org/10.17305/bjbms.2020.4271
Contact the author
Email
Message
The publishing house "Media Sphere" does not provide treatment services, does not give recommendations on contacting medical institutions and specific specialists, on the prescription of medicines and dietary supplements.

Email Confirmation

An email was sent to test@gmail.com with a confirmation link. Follow the link from the letter to complete the registration on the site.

Email Confirmation

Contact us
If you have any questions, complaints or suggestions, please use this feedback form.
Email
Message
The publishing house "Media Sphere" does not provide treatment services, does not give recommendations on contacting medical institutions and specific specialists, on the prescription of medicines and dietary supplements.
Submit Application

You can become an author of one of our magazines, send a request and we will consider it in during the day.

Name
Phone
E-mail
Message
Login
Registration
E-mail
Password
Forgot Password?

Log in to the site using your account in one of the services

Password recovery
E-mail
Login
Register

We use cооkies to improve the performance of the site. By staying on our site, you agree to the terms of use of cооkies. To view our Privacy and Cookie Policy, please. click here.