The use of the microbial and entomological data for the diagnostics of prescription of death coming

Prikhod’ko A.N.

Karelian Bureau of Forensic Medical Expertise, Petrozavodsk, Republic of Karelia, Russia, 185003

Lavrukova O.S.

Petrozavodsk State University, Petrozavodsk, Republic of Karelia, Russia, 185910

Lyabzina S.N.

Petrozavodsk State University, Petrozavodsk, Republic of Karelia, Russia, 185910

Sidorova N.A.

Petrozavodsk State University, Petrozavodsk, Russia, 185910

Popov V.L.

Kafedra sudebnoĭ meditsiny i pravovedeniia Sankt-Peterburgskogo gosudarstvennogo meditsinskogo universiteta im. akad. I.P. Pavlova

The use of the microbial and entomological data for the diagnostics of prescription of death coming

Authors:

Prikhod’ko A.N., Lavrukova O.S., Lyabzina S.N., Sidorova N.A., Popov V.L.

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Journal: Forensic Medical Expertise. 2018;61(6): 52‑56

DOI: 10.17116/sudmed20186106152

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To cite this article:

Prikhod’ko AN, Lavrukova OS, Lyabzina SN, Sidorova NA, Popov VL. The use of the microbial and entomological data for the diagnostics of prescription of death coming. Forensic Medical Expertise. 2018;61(6):52‑56. (In Russ.)
https://doi.org/10.17116/sudmed20186106152

Подписка 2025-2 (Forensic Medical Expertise)

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The objective of the present study was to demonstrate the possibility of the diagnostics of prescription of death coming (PDC) based on the results of the studies with the application of the entomological and microbiological methods. The entomological materials included larvae of two dipteran species Stearibia nigriceps (Piophilidae) and Fannia vesparia (Fanniidae) collected from bone tissue fragments and the bony bed. The detailed investigation of the succession of necrophilic insects taking place in the course of decomposition of organic matter and the development of the preimaginal stages of the dipterans made it possible to determine the duration of the period during which the corpse remained in the ambient environment. The microbiological studies made it possible to determine the composition of necrobiome of the bone remains. It included, among other things, the association of soil bacterial forms belonging to the orders Actinomycetales, Pseudomonadales, and Aeromonadales in the combination with the fungi of the order Endomycetales. The enteral microorganisms that trigger putrefaction at the early stages of corpse decomposition were totally absent in the remains that hosted soil microorganisms destroying the hard tissues. The predominance of Actinobacteria in the samples of bone remains gave evidence of active disintegration of the skeletal tissues by the bacterial species inhabiting the local soil milieu. The study demonstrated that the results of the analysis of the microbial constituent of the washouts from the surfaces of the skull and the left upper arm bone of the human corpse were consistent with the conclusion about the prescription of death coming based on the entomological data. It is agued that the development of the methods for the diagnostics of prescription of death coming, including those to be used for the examination of the skeletonized corpses, based on the results of the entomological and microbiological investigations has good prospects as an area of research in the field of forensic medicine.

Keywords:

bone remainss

putrefaction

sceleytonized corpses

prescription of death coming

forensic entomology

necrophilic dipterans

necrobiome

microbiological and entomological investigations

Authors:

Prikhod’ko A.N.

Karelian Bureau of Forensic Medical Expertise, Petrozavodsk, Republic of Karelia, Russia, 185003

Lavrukova O.S.

Petrozavodsk State University, Petrozavodsk, Republic of Karelia, Russia, 185910

Lyabzina S.N.

Petrozavodsk State University, Petrozavodsk, Republic of Karelia, Russia, 185910

Sidorova N.A.

Petrozavodsk State University, Petrozavodsk, Russia, 185910

Popov V.L.

Kafedra sudebnoĭ meditsiny i pravovedeniia Sankt-Peterburgskogo gosudarstvennogo meditsinskogo universiteta im. akad. I.P. Pavlova

List of references:

Dobryak VI. Sudebno-meditsinskaya expertiza skeletirovannogo trupa. Kiev: Gos. med. izdatel’stvo USSR. 1960. (In Russ.)
Evgen’ev-Tish EM. Ustanovlenie davnosti smerti v sudebno-meditsinskoi praktike. Kazan’: Kazansk. gos. med. institut, 1963. (In Russ.)
Solokhin AA, Solokhin YuA. Rukovodstvo po sudebno-meditsinskoi expertize trupa. M.: RMAPO, 1997. (In Russ.)
Samishchenko SS. Sudebnaya meditsina: uchebnik dlya vuzov. M.: Yurait, 2010. (In Russ.)
Lyubovitskii AV. Medico-kriminalisticheskie aspekty osmotra trupa: ucheb.-metod. posobie. Izhevsk: Jus est, 2010. (In Russ.)
Marchenko MI. Effect of Climatic Factors on Duration of the Biological Decomposition of Cadaver by Necrobiont Insect in Northwestern European Part of Russia. Entomologicheskoe obozrenie. 1992;71(3):557-568. (In Russ.)
Kan VB, Belikov IE. Sudebnaya meditsina: Kurs lektsii. Ekaterinburg: Izdatel’stvo Ural’skogo yuridicheskogo instituta MVD Rossii, 2002. (In Russ.)
Marchenko MI, Kononenko VI. Prakticheskoe rukovodstvo po sudebnoi entomologii. Pod red. Rubezhanskogo AF. Khar’kov. 1991.(In Russ.)
Shevchenko IN, Golubovich LL, Kurtev AV. The dynamic of putrefaction of death body. Sudovo-medichna expertiza. 2012;5:26-29. (In Russ.)
Bogomolov DV, Targashin AV, Pavlova AZ, Denisova OP, Amanmuradov AKh, Piryazeva EA, Bobrov AP. Primenenie mikologicheskikh i entomologicheskikh issledovanii pri provedenii sudebno-meditsinskoi ekspertizy trupa dlya ustanovleniya davnosti nastupleniya smerti i vozmozhnosti posmertnogo peremeshcheniya tela: Metodicheskie rekomendatsii. M. 2014. (In Russ.)
Lopatenok AA, Boiko LP, Budyakov OS. The case of using the corpse’s fauna for establishing the time of death. Sudebno-meditsinskaya expertiza. 1964;1:47-50. (In Russ.)
Rubezhanskii AF. Opredelenie po kostnym ostankam davnosti zakhoroneniya trupa. M.: Meditsina, 1978. (In Russ.)
Nainis I-VI, Marchenko MI, Kazak AN. A calculation method for estimating by enthomofauna the period during which the body had remained in the place where it was found. Sudebno-meditsinskaya expertiza. 1982;25(4):21-23.(In Russ.)
Starkeby M. Dead larvae of Cynomya mortuorum (L.) (Diptera, Calliphoridae) as indicators of the postmortem interval — a case history from Norway. Forensic Science International. 2001;129:77-78.
Popov VL, Lavrukova OS, Prikhodko AN, Lyabzina SN. The timing of the settlement of a corpse necrophilic fly Protophormia terraenovae (Diptera, Calliphoridae) to determine the duration of postmortem interval. Vestnik sudebnoi meditsiny. 2016;3:4-8. (In Russ.)
Damann FE, Williams D, Layton A. Potential use of bacterial community succession in decaying human bone for estimating postmortem intervals. Journal of Forensic Science. 2015;60:844-850. https://https://doi.org/10.1111/1556-4029.12744
Matuszewski S, Bajerlein D, Konwerski S, Szpila K. An initial study of insect succession and carrion decomposition in various forest habitats of Central Europe. Forensic Science International. 2008;180:61-69. https://https://doi.org/10.1016/j.forsciint.2008.06.015
Smith KGV. A manual of forensic entomology. London: Trustees of the British Museum. 1986.
Vinogradova EB, Marchenko MI. The use of temperature parameters of ely growth in the medicolegal practice. Sudebno-meditsinskaya ekspertiza. 1984;27(1):16-19. (In Russ.)
Kratkii opredelitel’ bakterii Bergi. Pod red. Khoulta Dzh. M.: Mir, 1980. (In Russ.)
Litvinov MA. Opredelitel’ mikroskopicheskikh pochvennykh gribov. L.: Nauka. 1967. (In Russ.)
Hyde ER, Haarmann DP, Petrosino JF, Lynne AM, Bucheli SR. Initial insights into bacterial succession during human decomposition. International Journal of Legal Medicine. 2014:1-14. https://https://doi.org/10.1007/s00414-014-1128-4
Damann FE, Carter DO. Human decomposition ecology and postmortem microbiology. In: James P, Symes S, Ropper C. (eds.). A Manual of Forensic Taphonomy. Boca Raton: CRC Press; 2013;37-49.
Evans WED. The Chemistry of Death. Springfield: Charles C. Thomas. 1963.
Pinheiro J. Decay process of a cadaver. In: Schmitt A, Cunha E, Pinheiro J. (eds). Forensic anthropology and medicine. New Jersey: Humana Press; 2006;85-116.
Parkinson RA. Bacterial Communities Associated with Human Decomposition. PhD thesis, Victoria University of Wellington, 2009.
Parkinson RA, Dias KR, Horswell J, Greenwood P, Banning N, Tibbett M, Vass AA. Microbial community analysis of human decomposition in soil. In: Ritz K, Dawson LA, Miller D. (eds.). Criminal and Environmental Soil Forensics. New York: Springer; 2009;379-394.
Damann FE. Human Decomposition Ecology at the University of Tennessee Anthropology Research Facility: Dis... PhD University of Tennessee, 2010.
Moorhead DL, Sinsabaugh RL. A theoretical model of litter decay and microbial interaction. Ecological Monographs. 2006;2(76):151-174. https://https://doi.org/10.1890/0012-9615(2006)076[0151:atmold]2.0.co;2
Turnbaugh PJ, Ley RE, Hamady M, Fraser-Liggett C, Knight R, Gordon JI. The human microbiome project. Nature. 2007;7164(449):804-810. https://https://doi.org/10.1038/nature06244
Costello EK, Lauber CL, Hamaday M, Fierer N, Gordon JI, Knight R. Bacterial community variation in human body habitats across space and time. Science. 2009;5960(326):1694-6167. https://https://doi.org/10.1126/science.1177486

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