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Zorina O.A.

GBOU VPO "Pervyĭ MGMU imeni I.M. Sechenova"

Petrukhina N.B.

Kafedra stomatologii FPPOV GBOU VPO "Pervyĭ Moskovskiĭ gosudarstvennyĭ meditsinskiĭ universitet im. I.M. Sechenova" Minzdrava RF

Basova А.А.

Department of Stomatology, Institute of Continuous Education, I.M. Sechenov First Moscow State Medical University, Moscow

Shibaeva A.V.

Institut biokhimicheskoĭ fiziki im. N.M. Émanuélia RAN, Moskva;
Institut poliomielita i virusnykh éntsefalitov im. M.P. Chumakova RAMN, Moskva

Trubnikova E.V.

Kurskiĭ gosudarstvennyĭ universitet, Kursk

Shevelev A.B.

Institut poliomielita i virusnykh éntsefalitov im. M.P. Chumakova RAMN, Moskva

Identification of key markers of normal and pathogenic microbiota determining health of periodontium by NGS-sequencing 16S-rDNA libraries of periodontal swabs

Authors:

Zorina O.A., Petrukhina N.B., Basova А.А., Shibaeva A.V., Trubnikova E.V., Shevelev A.B.

More about the authors

Journal: Stomatology. 2014;93(6): 25‑31

DOI: 10.17116/stomat201493625-31

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Table of contents

IDENTIFICATION OF KEY MARKERS OF NORMAL AND PATHOGENIC MICROBIOTA DETERMINING HEALTH OF PERIODONTIUM BY NGS-SEQUENCING 16S-RDNA LIBRARIES OF PERIODONTAL SWABS
IDENTIFICATION OF KEY MARKERS OF NORMAL AND PATHOGENIC MICROBIOTA DETERMINING HEALTH OF PERIODONTIUM BY NGS-SEQUENCING 16S-RDNA LIBRARIES OF PERIODONTAL SWABS

To cite this article:

Zorina OA, Petrukhina NB, Basova АА, Shibaeva AV, Trubnikova EV, Shevelev AB. Identification of key markers of normal and pathogenic microbiota determining health of periodontium by NGS-sequencing 16S-rDNA libraries of periodontal swabs. Stomatology. 2014;93(6):25‑31.
https://doi.org/10.17116/stomat201493625-31

Подписка 2026 Стоматология (Stomatology)
 
МСФ на ЯМ
Использование инфографики авторами научных работ
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Abstract:

By using NGS-sequencing libraries of DNA from periodontal swabs with primers specific to V6 region of 16S rDNA prevalence of bacterial genera and species in periodontal microbiota of patients with aggressive periodontitis and healthy donors was analyzed. Six genera of putative periodontal protectors and eight periodontal pathogens were identified with respect to aggressive (but not chronic) periodontitis. Statistically relevant over-colonization by general Porphyromonas, Treponema, Synergistes, Tannerella, Filifactor, Ruminococcus, Parvimonas and Mycoplasma was found to be associated with the condition. From these, only three genera Porphyromonas, Treponema and Tannerella are traditionally considered as periodontal pathogens. Statistically confidential over-colonization by genus Veillonella was found in healthy patients. This genus should be considered as a relevant marker of a healthy periodontium. Genera Streptococcus, Bergeyella, Granulicatella, Kingella and Corynebacterium may be considered as putative periodontal protectors. Comparison of data of NGS-sequencing and real-time PCR demonstrated a good agreement if different PCR efficiency using independent primer pairs is taken into account.

Keywords:

periodontium
aggressive periodontitis
periodontal pathogen
genom
16S rRNA
16S rDNA
PCR
RT-PCR
sequencing
NGS

Authors:

Zorina O.A.

GBOU VPO "Pervyĭ MGMU imeni I.M. Sechenova"

Petrukhina N.B.

Kafedra stomatologii FPPOV GBOU VPO "Pervyĭ Moskovskiĭ gosudarstvennyĭ meditsinskiĭ universitet im. I.M. Sechenova" Minzdrava RF

Basova А.А.

Department of Stomatology, Institute of Continuous Education, I.M. Sechenov First Moscow State Medical University, Moscow

Shibaeva A.V.

Institut biokhimicheskoĭ fiziki im. N.M. Émanuélia RAN, Moskva;
Institut poliomielita i virusnykh éntsefalitov im. M.P. Chumakova RAMN, Moskva

Trubnikova E.V.

Kurskiĭ gosudarstvennyĭ universitet, Kursk

Shevelev A.B.

Institut poliomielita i virusnykh éntsefalitov im. M.P. Chumakova RAMN, Moskva

Close metadata

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