Innovative experimental approaches to improving anti-infective safety measures and disinfection in the practice of an orthopedic dentist

Akavov AN, Arutyunov DS, Deshev AV, Karpova VM, Tsareva EV, Podporin MS, Tsarev VN. Innovative experimental approaches to improving anti-infective safety measures and disinfection in the practice of an orthopedic dentist. Russian Journal of Stomatology. 2023;16(4):36‑43. (In Russ.)
https://doi.org/10.17116/rosstomat20231604136

Подписка 2026 Российская стоматология (Russian Journal of Stomatology)

Использование инфографики авторами научных работ

Close metadata

RELEVANCE

It is believed that on average to 9% of hospital patients annually suffer from the development and severe course of infectious complications caused by infectious agents associated with the provision of medical care (ISMP). Therefore, the solution of infectious safety issues, including in the practical work of a dentist, is an extremely urgent problem.

OBJECTIVE

To apply an innovative method of experimental microbiological study of programmable cultivation to select optimal disinfectants and methods of effective disinfection of dental impressions.

MATERIAL AND METHODS

The microbiota was analyzed on alginate dental impressions (30 impressions). Then, in an in vitro experiment, the effect of preparations from the group of quaternary ammonium derivatives (QAD) recommended for chemical disinfection was evaluated: benzalkonium chloride (BAC), cytilpyridinium chloride (CPC), benzyldimethyl[3-(myristoylamino)propyl] ammonium chloride (BMC).

RESULTS

The bactericidal concentration at which the growth of aerobic (S. aureus) and anaerobic bacteria (F. necroforum) was completely blocked was 0.05% for BAC, 0.05—0.025% for CPC and 0.025—0.012% for BMC. Activity against strains of yeast fungi C. albicans and C. krusei was slightly lower and amounted to 0.1 and 0.2%, respectively, in BMC.

CONCLUSION

The bactericidal concentration of BMC was 2 times lower compared to all tested strains, which can be noted as an important advantage of the drug.

Keywords:

infections related to medical care

chemical disinfection

dental impressions

bioreactor

quaternary ammonium derivatives

Authors:

Akavov A.N.

Evdokimov Moscow State University of Medicine and Dentistry;
Dagestan State Medical University

ORCID: 0009-0000-6987-6308

Arutyunov D.S.

Evdokimov Moscow State University of Medicine and Dentistry

ORCID: 0009-0002-9325-2751

Deshev A.V.

Evdokimov Moscow State University of Medicine and Dentistry;
Berbekov Kabardino-Balkar State University

ORCID: 0009-0005-0583-5044

Karpova V.M.

Evdokimov Moscow State University of Medicine and Dentistry

SPIN RSCI: 5404-1770
ORCID: 0000-0003-1003-6667

Tsareva E.V.

Evdokimov Moscow State University of Medicine and Dentistry

SPIN RSCI: 8851-3338
ORCID: 0000-0001-8600-0447

Podporin M.S.

Evdokimov Moscow State University of Medicine and Dentistry

ORCID: 0000-0001-6785-0016

Tsarev V.N.

Evdokimov Moscow State University of Medicine and Dentistry

ORCID: 0000-0002-3311-0367

Received:

22.05.2023

Accepted:

11.07.2023

Close metadata

References:

Arutyunov SD, Yushchuk ND, Martynov YuV, Kukhtevich EV. Dental medical organization: sanitary-hygienic and anti-epidemic regime. M.: Practical Medicine; 2021. (In Russ.).
Rutala WA, Weber DJ. Guideline for disinfection and sterilization in healthcare facilities, 2008. Infection Control and Hospital Epidemiology. 2008;18:240-264.
Nyhsen CM, Humphreys H, Koerner RJ, et al. Infection prevention and control in ultrasound — best practice recommendations from the European Society of Radiology Ultrasound Working Group. Insights Imaging. 2017;8:523-535.
Pereverzeva EV, Melnichuk VI. Disinfection. Sterilization: Methodological recommendations. Minsk: BSMU; 2019. (In Russ.).
Tsarev VN, Ippolitov EV, Lashchenko ES, Arutyunov SD. Technologies of chemical disinfection of dental instruments with the help of formaldehyde-free disinfectants of a new generation. Russian Journal of Stomatology. 2014;7(3):43-48. (In Russ.).
Kamilov RI. Application of «Okadez M» for chemical disinfection of dental impressions: Diss. ... kand. med. nauk. M.: MGMSU; 2004. (In Russ.).
Sarkisyan MS, Ushakov RV, Tsarev VN. Microbial contamination of casts in the clinic of orthopedic dentistry. Dentistry for Everyone. 2000;2:20. (In Russ.).
Sakieva ZV. Disinfection of dental impressions with non-aldehyde disinfectants of a new generation: Diss. ... kand. med. nauk. M.: MGMSU; 2015. (In Russ.).
Arutyunov SD, Khasigova ZV, Kamilov RI, et al. Comparative evaluation of the effect of new chemical disinfectants on the physical and microbiological characteristics of dental impressions. Russian Dental Journal. 2013;5:34-38. (In Russ.).
Tsarev VN, Arutyunov SD, Ostroukhova AA, et al. The effectiveness of the combined effect of chemical disinfectant and plasma sterilization on dental instruments and impression materials. Dentistry. 2002;81(4):24-28. (In Russ.).
Rutala WA, Weber DJ. ERCP scopes: what can we do to prevent infections? Infect Control Hosp Epidemiol. 2015;36(6):643-648.
Qiu Q-Q, Sun W-Q, Connor J. Sterilization of biomaterials of synthetic and biological origin. Comprehensive Biomaterials. 2011;4:127-144.
Salimon AI, Statnik ES, Kan Yu, et al. Comparative study of biomaterial surface modification due to subcritical CO2 and autoclave disinfection treatment. J Supercritical Fluids. 2022;191(105789). https://doi.org/10.1016/j.supflu.2022.105789
Arutyunov SD, Yanushevich OO, Korsunsky AM, et al. Comparative analysis of the effectiveness of modern methods of sterilization of instruments and the place of gas-dynamic treatment with carbon dioxide. Russian Journal of Stomatology. 2022;15(1):12-19. (In Russ.). https://doi.org/10.17116/rosstomat20221501112
Trezubov VN, Arutyunov SD, Mishnev LM, et al. Clinical dentistry. Hospital course. Vol. I: Organizational and preventive aspects of clinical dentistry. 3rd edn. M.: Practical Medicine; 2020. (In Russ.).
Willey JM, Woolverton CJ, Sherwood L. Prescott’s Principles of Microbiology. 2008:126-153.
Akimkin VG, Kuzin SN, Kolosovskaya EN, et al. Characteristics of the epidemiological situation of COVID-19 in St. Petersburg. Zhurnal microbiologii, epidemiologii i immunobiologii. 2021;98(5):497-511. (In Russ.).
Chen Z, Wherry JE. T cell responses in patients with COVID-19. Nat Rev Immunol. 2020;20(9):529-536. https://doi.org/10.1038/s41577-020-0402-6
Ivanisenko NV, Seyrek K, Kolchanov NA, et al. The role of death domain proteins in host response upon SARS-CoV-2 infection: modulation of programmed cell death and translational applications. Cell Death Discov. 2020;6:101. https://doi.org/10.1038/s41420-020-00331-w
Khrapunova IA, Shestopalov NV. The role of disinfection measures in the prevention of infections associated with the provision of medical care (based on the materials of new regulatory and methodological documents). Disinfection Business. 2022;2(120):30-37. (In Russ.). https://doi.org/10.35411/2076-457X-2022-2-30-37
Podporin MS, Tsarev VN, Ippolitov EV, et al. Experimental substantiation of the development of the dosage form of lactoferrin with enamel matrix derivatives for use in periodontology. Clinical Dentistry (Russia). 2022;25(4):74-80. (In Russ.). https://doi.org/10.37988/1811-153X_2022_4_74
Microbiology, virology, immunology of the oral cavity: Textbook. Edited by Tsarev VN. 2nd edn., reprint. and add. M.: GEOTAR-Media; 2019. (In Russ.).
Balmasova IP, Tsarev VN, Yanushevich OO, et al. Microecology of periodontal disease. The relationship of local and systemic effects: monograph. M.: Practical Medicine; 2021. (In Russ.).
Frolov GA, Abdukadirov AA, Lundovskikh IA, et al. Experimental evaluation in in vitro and in vivo experiments of the sensitivity of Yersinia pseudotuberculosis bacteria to antimicrobial compositions based on copper nanoparticles. Disinfection Business. 2023;1(123):5-23. (In Russ.). https://doi.org/10.35411/2076-457X-2023-1-5-23
Kuzin VV, Kolupaeva NV, Kuzina ES, Potapov VD. Investigation of decontaminating activity of disinfectants of various classes in relation to nucleic acids. Disinfection Business. 2022;1(119):14-20. (In Russ.). https://doi.org/10.35411/2076-457X-2022-1-14-20