Prevalence of antibiotic resistance among bacterial strains isolated in chronic periodontitis and in healthy people

Arutyunyan A.A.; Tsareva T.V.; Ippolitov E.V.; Sarkisyan M.A.; Ponomareva A.G.

doi:https://doi.org/10.17116/rosstomat20231601119

Arutyunyan A.A.

Evdokimov Moscow State University of Medicine and Dentistry

Tsareva T.V.

Moscow State University of Medicine and Dentistry

Ippolitov E.V.

Evdokimov Moscow State University of Medicine and Dentistry

Sarkisyan M.A.

Evdokimov Moscow State University of Medicine and Dentistry

Ponomareva A.G.

Evdokimov Moscow State University of Medicine and Dentistry

Prevalence of antibiotic resistance among bacterial strains isolated in chronic periodontitis and in healthy people

Authors:

Arutyunyan A.A., Tsareva T.V., Ippolitov E.V., Sarkisyan M.A., Ponomareva A.G.

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Journal: Russian Journal of Stomatology. 2023;16(1): 19‑23

DOI: 10.17116/rosstomat20231601119

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To cite this article:

Arutyunyan AA, Tsareva TV, Ippolitov EV, Sarkisyan MA, Ponomareva AG. Prevalence of antibiotic resistance among bacterial strains isolated in chronic periodontitis and in healthy people. Russian Journal of Stomatology. 2023;16(1):19‑23. (In Russ.)
https://doi.org/10.17116/rosstomat20231601119

Подписка 2026 Российская стоматология (Russian Journal of Stomatology)

Использование инфографики авторами научных работ

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THE AIM OF THE STUDY

Was to establish the prevalence of strains of microorganisms resistant to antibacterial drugs isolated from subgingival dental plaques of periodontitis patients in the acute and remission stages.

MATERIALS AND METHODS

For the study, patients (90 people) were selected who were diagnosed with chronic periodontitis (K05.3) as a result of clinical examination and subsequent treatment on the basis of the Clinical Center of Dentistry of the Moscow State Medical University named after A.I. Evdokimov of the Ministry of Health of the Russian Federation and in the dental departments of the Moscow Medical Institution (№23, 29). The research methods included culture (disco-diffusion method) and molecular biological technologies (PCR), which allows to identify resistant strains of bacteria isolated from subgingival biofilm.

RESULTS

Based on the inclusion/exclusion criteria, 3 comparison groups were formed: 1 — chronic periodontitis in the acute phase (they applied for the main dental disease); 2 — chronic periodontitis in the remission phase (they applied for dental implantation); 3 — practically healthy with intact periodontitis (they applied for therapeutic and preventive measures). Analysis of the frequency of detection of R-genes in general showed that the markers ermB and Mef, Van A, B (40—50%), as well as Bla DHA were most often detected in representatives of the microbiota in patients with chronic periodontitis in the acute phase. Detection of CTX-M, Tet M and INT was at an average level (approximately ¹/₃ of the strains). The minimum level of detection of genetic markers of resistance was noted for the markers Mes A and Tet Q (17.4%). A similar level was in the remission phase. A lower level was noted in patients with intact periodontal disease.

CONCLUSION

The results obtained allow us to justify the return to treatment regimens using doxycycline, as one of the most adequate domestic tetracycline drugs.

Keywords:

chronic periodontitis

disco-diffusion method

PCR

R-genes

Authors:

Arutyunyan A.A.

Evdokimov Moscow State University of Medicine and Dentistry

Tsareva T.V.

Moscow State University of Medicine and Dentistry

Ippolitov E.V.

Evdokimov Moscow State University of Medicine and Dentistry

Sarkisyan M.A.

Evdokimov Moscow State University of Medicine and Dentistry

Ponomareva A.G.

Evdokimov Moscow State University of Medicine and Dentistry

Received:

21.11.2022

Accepted:

12.12.2022

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References:

Yanushevich OO, Akhmedov GD, Panin AM, et al. Microecology of the oral cavity and infectious and inflammatory complications in surgical dentistry (monography). M.: Practical Medicine; 2019. (In Russ.).
Kuzmina EM, Yanushevich OO, Kuzmina IN. Dental morbidity of the Russian population. Epidemiological dental examination of the population of Russia. M.: MGMSU; 2019. (In Russ.).
Makeeva IM, Daurova FYu, Byakova SF, et al. Sensitivity of microbial associations of periodontal pocket exudate and odontogenic focus to antibacterial drugs. Dentistry. 2016;95(3):26-30. (In Russ.).
Ushakov RV, Tsarev VN. Antimicrobial therapy in dentistry. Principles and algorithms (a guide for doctors). M.: Practical Medicine; 2019. (In Russ.).
Chaplin AV, Korzhanova M, Korostin DO. Identification of bacterial antibiotic resistance genes in genome-wide sequencing data (literature review). Clinical laboratory diagnostics. 2021;11(66):684-688. (In Russ.). https://doi.org/10.51620/0869-2084-2021-66-11-684-688
Caton JG, Armitage G, Berglundh T, et al. A new classification scheme for periodontal and peri-implant diseases and conditions — Introduction and key changes from the 1999 classification. J Periodontol. 2018;89(suppl 1):1-8. https://doi.org/10.1111/jcpe.12935
Graetz C, Mann L, Krois J, Salzer S, Kahl M, Springer C, et al. Comparison of periodontitis patients’ classification in the 2018 versus 1999 classification. J Clin Periodontol. 2019;46(9): 908-917. https://doi.org/10.1111/jcpe.13157
Ushakov RV, Nuruev NN, Ushakova TV, et al. Combined antimicrobial chemotherapy (fluoroquinolones and imidazoles) in the complex treatment of inflammatory periodontal diseases. Clinical dentistry. 2021;1(97):60-65. (In Russ.).
Ushakov RV, Tsarev VN, Robustova TG, et al. Substantiation of algorithms of antimicrobial chemotherapy in the complex treatment of head and neck phlegmon. Clinical dentistry. 2021;24(3):14-21. (In Russ.). https://doi.org/10.37988/1811-153_2021_3_14
Ilyin VK, Solovyova ZO, Giesinger OA, et al. Comparison of the PCR diagnostic method and the method of mass spectrometry of microbial markers in relation to the assessment of oral microbiota. Clinical laboratory diagnostics. 2022;8(67):484-488. (In Russ.). https://doi.org/10.51620/0869-2084-2022-67-8-484-488
Samusenkov VO, Tsarev VN, Ippolitov EV, et al. Results of clinical and laboratory studies in the complex treatment of chronic periodontitis of moderate severity using photodynamic therapy. Russian dentistry. 2021;14(3):3-8. (In Russ.). https://doi.org/10.17116/rosstomat 2021140313
Balmasova IP, Tsarev VN, Yanushevich OO, et al. Microecology of periodontal disease. The relationship of systemic and local effects (monography). M.: Practical Medicine; 2021. (In Russ.).
Ilyin VK, Tsarev VN, Skedina MA, et al. The state of barriers to microbial colonization of the dental system in simulated weightlessness. Pathological physiology and experimental therapy. 2020;64(1):76-83. (In Russ.).
Arredondo A, Blanc V, Mor C, et al. Resistance to β-lactams and distribution of β-lactam resistance genes in subgingival microbiota from Spanish patients with periodontitis. Clin Oral Investig. 2020. https://doi.org/10.1007/s00784-020-03333-1
Broom LJ. The sub-inhibitory theory for antibiotic growth promoters. Poult Sci. 2017;96(9):3104-3108. [PubMed] [Google Scholar].
Lu L, Liu J, Li Z, et al. Occurrence and distribution of tetracycline antibiotics and resistance genes in longshore sediments of the three gorges reservoir. China. Front Microbiol. 2018;9:1911. [PMC free article] [PubMed] [Google Scholar].
Collins JR, Arredondo A, Roa A, et al. Periodontal pathogens and tetracycline resistance genes in subgingival biofilm of periodontally healthy and diseased Dominican adults. Clin Oral Investig. 2016;20(2):349-356. [PMC free article] [PubMed] [Google Scholar].
Checchi L, Gatto MR, Checchi V, et al. Bacteria prevalence in a large Italian population sample: a clinical and microbiological study. J Biol Regul Homeost Agents. 2016;30(S2):199-208. [PubMed] [Google Scholar].
Larsen JM. The immune response to Prevotella bacteria in chronic inflammatory disease. Immunology. 2017;151(4):363-374. [PubMed] [Google Scholar].
Ioannidis I, Sakellari D, Spala A, et al. Prevalence of tetM, tetQ, nim and blaTEM genes in the oral cavities of Greek subjects: a pilot study. J Clin Periodontol. 2009;36(7):569-574. [PubMed] [Google Scholar].
Kim S-M, Kim HC, Lee S-WS. Characterization of antibiotic resistance determinants in oral biofilms. J Microbiol. 2011;49(4):595-602. [PubMed] [Google Scholar].