Objective - to investigate the association of the single nucleotide polymorphisms (SNPs) rs10757278 and rs1333049 of the 9p21.3 locus with the long-term outcomes of myocardial infarction (MI) during a two-year follow-up. The investigation enrolled of 477 Caucasian patients aged ≤65 years (mean age 52.36±7.45 years) with myocardial infarction (MI) who had been discharged from hospital; all the participants wrote informed consent. There was a male predominance [n=390 (81.8%)] in the study group. Genomic DNA was extracted from venous blood leukocytes using phenol-chloroform extraction. Gene polymorphism was tested using real-time polymerase chain reaction according to the manufacturer's protocol (primers and probes TaqMan, AB 7900HT). The prospective follow-up after MI lasted 2 years. There was no association of the polymorphic variants of the locus 9p21.3 with in-hospital mortality and MI outcomes (mortality, recurrent MI, hospitalization due to unstable angina pectoris, and percutaneous coronary interventions) during long-term (6-, 12-, 24-month) follow-up periods. The risk genotypes of the SNPs rs10757278 and rs1333049 of the locus 9p21.3 exert no impact on MI outcomes.