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Isaeva O.V.
Russian Medical Academy of Continuous Professional Education;
Mechnikov Research Institute for Vaccines and Sera
Karlsen A.A.
Russian Medical Academy of Continuous Professional Education;
Mechnikov Research Institute for Vaccines and Sera
Ilchenko L.Y.
Mechnikov Research Institute for Vaccines and Sera;
Pirogov Russian National Research Medical University
Sleptsova S.S.
North-Eastern Federal University, Medical Institute
Saryglar A.A.
Kyzyl Infectious Hospital»
Poryvaeva V.A.
JSC «Vector-Best», Research and Production area
Mosina Ya.D.
JSC «Vector-Best», Research and Production area
Agafonova O.A.
JSC «Vector-Best», Research and Production area
Mogilnykh A.K.
JSC «Vector-Best», Research and Production area
Kyuregyan K.K.
Russian Medical Academy of Continuous Professional Education;
Mechnikov Research Institute for Vaccines and Sera
Mikhailov M.I.
Russian Medical Academy of Continuous Professional Education;
Mechnikov Research Institute for Vaccines and Sera
Hepatitis B virus genotypes in patients with hepatitis D, determined by the panel of own development monoclonal antibodies
Journal: Molecular Genetics, Microbiology and Virology. 2022;40(2): 43‑50
Views: 2515
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To cite this article:
Bezuglova LV, Isaeva OV, Karlsen AA, et al. . Hepatitis B virus genotypes in patients with hepatitis D, determined by the panel of own development monoclonal antibodies. Molecular Genetics, Microbiology and Virology.
2022;40(2):43‑50. (In Russ.)
https://doi.org/10.17116/molgen20224002143
Direct genotyping of hepatitis B virus (HBV) in samples from patients with hepatitis delta can be impossible due to the undetectable concentration of HBV DNA. A sufficient amount of surface HBV protein (HBsAg) in such samples makes it possible to determine HBV genotype using enzyme-linked immunosorbent assay (ELISA) of this antigen with a panel of monoclonal antibodies (MAB).
To compare the results of HBV genotyping using the in-house MAB panel with the results of molecular analysis of samples from patients with chronic hepatitis B (CHB) and to determine HBV genotypes in samples from patients with hepatitis delta.
Total 122 serum samples from CHB patients from Yakutia and 211 serum samples from hepatitis delta patients from Yakutia (12 samples) and Tuva (199 samples) were collected. HBV serotypes/genotypes were determined by ELISA using the developed reagents. Molecular methods included the isolation of HBV DNA, amplification of the S gene region (713 nt), phylogenetic analysis of the nucleotide sequences.
In a group of samples from CHB patients positive for HBV DNA (86 samples) 95% (82/86) valid results were obtained using our MAB kit. The following genotypes were identified: A — 32 (39%), C — 3 (4%), D — 47 (57%). The results of HBV genotyping using two methods were identical for 81/82 (99%) samples. HBV genotyping using developed reagents gave 96.2% (203/211) of valid results in samples from patients with hepatitis delta compared to 3.8% obtained using standard molecular technic (p<0.001). Following HBV genotypes were identified: A — 17 samples (8.4%) and D — 186 samples (91.6%).
The developed test with MAB panel is able to reliably determine HBV genotype and has advantages over standard molecular methods for HBV genotyping in patients with hepatitis delta.
Keywords:
Authors:
Isaeva O.V.
Russian Medical Academy of Continuous Professional Education;
Mechnikov Research Institute for Vaccines and Sera
Karlsen A.A.
Russian Medical Academy of Continuous Professional Education;
Mechnikov Research Institute for Vaccines and Sera
Ilchenko L.Y.
Mechnikov Research Institute for Vaccines and Sera;
Pirogov Russian National Research Medical University
Sleptsova S.S.
North-Eastern Federal University, Medical Institute
Saryglar A.A.
Kyzyl Infectious Hospital»
Poryvaeva V.A.
JSC «Vector-Best», Research and Production area
Mosina Ya.D.
JSC «Vector-Best», Research and Production area
Agafonova O.A.
JSC «Vector-Best», Research and Production area
Mogilnykh A.K.
JSC «Vector-Best», Research and Production area
Kyuregyan K.K.
Russian Medical Academy of Continuous Professional Education;
Mechnikov Research Institute for Vaccines and Sera
Mikhailov M.I.
Russian Medical Academy of Continuous Professional Education;
Mechnikov Research Institute for Vaccines and Sera
Received:
12.08.2020
Accepted:
30.10.2020
List of references:
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