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Bezuglova L.V.

JSC «Vector-Best»

Isaeva O.V.

Russian Medical Academy of Continuous Professional Education;
Mechnikov Research Institute for Vaccines and Sera

Karlsen A.A.

Russian Medical Academy of Continuous Professional Education;
Mechnikov Research Institute for Vaccines and Sera

Ilchenko L.Y.

Mechnikov Research Institute for Vaccines and Sera;
Pirogov Russian National Research Medical University

Sleptsova S.S.

North-Eastern Federal University, Medical Institute

Saryglar A.A.

Kyzyl Infectious Hospital»

Poryvaeva V.A.

JSC «Vector-Best», Research and Production area

Mosina Ya.D.

JSC «Vector-Best», Research and Production area

Agafonova O.A.

JSC «Vector-Best», Research and Production area

Mogilnykh A.K.

JSC «Vector-Best», Research and Production area

Kyuregyan K.K.

Russian Medical Academy of Continuous Professional Education;
Mechnikov Research Institute for Vaccines and Sera

Mikhailov M.I.

Russian Medical Academy of Continuous Professional Education;
Mechnikov Research Institute for Vaccines and Sera

Netesov S.V.

Novosibirsk State University

Netesova I.G.

JSC «Vector-Best»

Hepatitis B virus genotypes in patients with hepatitis D, determined by the panel of own development monoclonal antibodies

Authors:

Bezuglova L.V., Isaeva O.V., Karlsen A.A., Ilchenko L.Y., Sleptsova S.S., Saryglar A.A., Poryvaeva V.A., Mosina Ya.D., Agafonova O.A., Mogilnykh A.K., Kyuregyan K.K., Mikhailov M.I., Netesov S.V., Netesova I.G.

More about the authors

Journal: Molecular Genetics, Microbiology and Virology. 2022;40(2): 43‑50

DOI: 10.17116/molgen20224002143

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Table of contents

HEPATITIS B VIRUS GENOTYPES IN PATIENTS WITH HEPATITIS D, DETERMINED BY THE PANEL OF OWN DEVELOPMENT MONOCLONAL ANTIBODIES
HEPATITIS B VIRUS GENOTYPES IN PATIENTS WITH HEPATITIS D, DETERMINED BY THE PANEL OF OWN DEVELOPMENT MONOCLONAL ANTIBODIES

To cite this article:

Bezuglova LV, Isaeva OV, Karlsen AA, et al. . Hepatitis B virus genotypes in patients with hepatitis D, determined by the panel of own development monoclonal antibodies. Molecular Genetics, Microbiology and Virology. 2022;40(2):43‑50. (In Russ.)
https://doi.org/10.17116/molgen20224002143

Подписка 2025-2 (Molecular Genetics, Microbiology and Virology)
 
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BACKGROUND

Direct genotyping of hepatitis B virus (HBV) in samples from patients with hepatitis delta can be impossible due to the undetectable concentration of HBV DNA. A sufficient amount of surface HBV protein (HBsAg) in such samples makes it possible to determine HBV genotype using enzyme-linked immunosorbent assay (ELISA) of this antigen with a panel of monoclonal antibodies (MAB).

PURPOSE

To compare the results of HBV genotyping using the in-house MAB panel with the results of molecular analysis of samples from patients with chronic hepatitis B (CHB) and to determine HBV genotypes in samples from patients with hepatitis delta.

MATERIAL AND METHODS

Total 122 serum samples from CHB patients from Yakutia and 211 serum samples from hepatitis delta patients from Yakutia (12 samples) and Tuva (199 samples) were collected. HBV serotypes/genotypes were determined by ELISA using the developed reagents. Molecular methods included the isolation of HBV DNA, amplification of the S gene region (713 nt), phylogenetic analysis of the nucleotide sequences.

RESULTS

In a group of samples from CHB patients positive for HBV DNA (86 samples) 95% (82/86) valid results were obtained using our MAB kit. The following genotypes were identified: A — 32 (39%), C — 3 (4%), D — 47 (57%). The results of HBV genotyping using two methods were identical for 81/82 (99%) samples. HBV genotyping using developed reagents gave 96.2% (203/211) of valid results in samples from patients with hepatitis delta compared to 3.8% obtained using standard molecular technic (p<0.001). Following HBV genotypes were identified: A — 17 samples (8.4%) and D — 186 samples (91.6%).

CONCLUSIONS

The developed test with MAB panel is able to reliably determine HBV genotype and has advantages over standard molecular methods for HBV genotyping in patients with hepatitis delta.

Keywords:

HBsAg
hepatitis B virus
genotype
monoclonal antibodies
enzyme-linked immunosorbent assay
hepatitis D

Authors:

Bezuglova L.V.

JSC «Vector-Best»

Isaeva O.V.

Russian Medical Academy of Continuous Professional Education;
Mechnikov Research Institute for Vaccines and Sera

Karlsen A.A.

Russian Medical Academy of Continuous Professional Education;
Mechnikov Research Institute for Vaccines and Sera

Ilchenko L.Y.

Mechnikov Research Institute for Vaccines and Sera;
Pirogov Russian National Research Medical University

Sleptsova S.S.

North-Eastern Federal University, Medical Institute

Saryglar A.A.

Kyzyl Infectious Hospital»

Poryvaeva V.A.

JSC «Vector-Best», Research and Production area

Mosina Ya.D.

JSC «Vector-Best», Research and Production area

Agafonova O.A.

JSC «Vector-Best», Research and Production area

Mogilnykh A.K.

JSC «Vector-Best», Research and Production area

Kyuregyan K.K.

Russian Medical Academy of Continuous Professional Education;
Mechnikov Research Institute for Vaccines and Sera

Mikhailov M.I.

Russian Medical Academy of Continuous Professional Education;
Mechnikov Research Institute for Vaccines and Sera

Netesov S.V.

Novosibirsk State University

Netesova I.G.

JSC «Vector-Best»

Received:

12.08.2020

Accepted:

30.10.2020

List of references:

  1. McMahon BJ. The influence of hepatitis B virus genotype and subgenotype on the natural history of chronic hepatitis B. Hepatology International. 2009;3(2):334-342.  https://doi.org/10.1007/s12072-008-9112-z
  2. Kao JH, Wu NH, Chen PJ, Lai MY, Chen DS. Hepatitis B genotypes and the response to interferon therapy. J Hepatol. 2000;33(6):998-1002. https://doi.org/10.1016/s0168-8278(00)80135-x
  3. Ozasa A, Tanaka Ya, Orito E, Sugiyama M, Kang J, Hige Sh, et al. Influence of Genotypes and Precore Mutations on Fulminant or Chronic Outcome of Acute Hepatitis B Virus Infection. Hepatology. 2006;44(2):326-334.  https://doi.org/10.1002/hep.21249
  4. Kramvis A. Genotypes and genetic variability of hepatitis B virus. Intervirology. 2014;57(3-4):141-150.  https://doi.org/10.1159/000360947
  5. Lin C-L, Kao J-H. Hepatitis B virus genotypes and variants. Cold Spring Harbor Perspectives in Medicine. 2015;5(5):a021436. https://doi.org/10.1101/cshperspect.a021436
  6. Kobayashi M, Suzuki F, Arase Y, Akuta N, Suzuki Y, Hosaka T, et al. Infection with hepatitis B virus genotype A in Tokyo, Japan during 1976 through 2001. J Gastroenterology. 2004;39(9):844-850.  https://doi.org/10.1007/s00535-004-1400-3
  7. Mayerat C, Mantegani A, Frei PC. Does hepatitis B virus (HBV) genotype influence the clinical outcome of HBV infection? J Viral Hepatitis. 1999;6(4):299-304.  https://doi.org/10.1046/j.1365-2893.1999.00174.x
  8. Halfon P, Bourlière M, Pol S, Benhamou Y, Ouzan D, Rotily M, et al. Multicentre study of hepatitis B virus genotypes in France: correlation with liver fibrosis and hepatitis B e antigen status. J Viral Hepatitis. 2006;13(5):329-335.  https://doi.org/10.1111/j.1365-2893.2005.00692.x
  9. Hsieh TH, Tseng TC, Liu CJ, Lai MY, Chen PJ, Hsieh HL, et al. Hepatitis B virus genotype B has an earlier emergence of lamivudine resistance than genotype C. Antiviral Therapy. 2009;14(8):1157-1163. https://doi.org/10.3851/imp1454
  10. Chan H L-Y, Wong ML, Hui AY, Hung L C-T, Chan F K-L, Sung J J-Y. Hepatitis B virus genotype C takes a more aggressive disease course than hepatitis B virus genotype B in hepatitis B e antigen-positive patients. J Clin Microbiol. 2003;41(3):1277-1279. https://doi.org/10.1128/jcm.41.3.1277-1279.2003
  11. Su CW, Huang YH, Huo TI, Shih HH, Sheen IJ, Chen SW, et al. Genotypes and viremia of hepatitis B and D viruses are associated with outcomes of chronic hepatitis D patients. Gastroenterology. 2006;130(6):1625-1635. https://doi.org/10.1053/j.gastro.2006.01.035
  12. Hughes SA, Wedemeyer H, Harrison PM. Hepatitis delta virus. Lancet. 2011;378(9785):73-85.  https://doi.org/10.1016/s0140-6736(10)61931-9
  13. Zachou K, Yurdaydin C, Drebber U, Dalekos GN, Erhardt A, Cakaloglu Y, et al. Quantitative HBsAg and HDV RNA levels in chronic delta hepatitis. Liver International. 2010;30(3):430-437.  https://doi.org/10.1111/j.1478-3231.2009.02140.x
  14. Usuda S, Okamoto H, Iwanari H, Baba K, Tsuda F, Miyakawa Y, et al. Serological detection of hepatitis B virus genotypes by ELISA with monoclonal antibodies to type-specific epitopes in the preS2-region product. J Virolog Methods. 1999;80:97-112.  https://doi.org/10.1016/S0166-0934(99)00039-7
  15. Bezuglova LV, Mosina YaD, Poryvaeva VA, Netesova IG. Determination of the genotype of hepatitis B virus and the HBsAg subtype by enzyme immunoassay. Sbornik trudov VIII Vserossiyskoy nauchno-prakticheskoy konferentsii «Molekulyarnaya diagnostika-2014» s mezhdunarodnym uchastiyem. M. 2014;1:124-125. (In Russ.).
  16. Bezuglova LV, Manuilov VA, Osipova LP, Mosina YaD, Poryvaeva VA, Agafonova OA, et al. The results of testing the laboratory version of the test kit for determination of HBsAg subtypes and hepatitis B virus genotypes in human blood plasma samples using ELISA. Molekuljarnaja genetika mikrobiologija i virusologija. 2020;38(4):188–195. (In Russ.). https://doi.org/10.17116/molgen202038041188
  17. ICTV 9th Report (2011). Accessed 15 July 2020. https://talk.ictvonline.org/ictv-reports/ictv_9th_report/reverse-transcribing-dna-and-rna-viruses-2011/w/rt_viruses/155/hepadnaviridae
  18. PhyML 3.0: new algorithms, methods and utilities. Aaccessed 15 July 2020. https://www.atgc-montpellier.fr/phyml/
  19. Posada D. jModelTest: phylogenetic model averaging. Molecular Biology and Evolution. 2008;25(7):1253-1256. https://doi.org/10.1093/molbev/msn083
  20. A fast implementation of aLRT in PhyML. Accessed 15 July 2020. https://www.atgc-montpellier.fr/phyml/alrt/
  21. https://medstatistic.ru/calculators/calchi.html
  22. Rizzetto M, Canese M, Arico S, Crivell O, Trepo C, Bonino F, et al. Immunofluorescence detection of new antigen—antibody system (delta/anti—delta) associated to hepatitis B virus in liver and in serum of HBsAg carriers. Gut. 1977;18(12):997-1003. https://doi.org/10.1136/gut.18.12.997
  23. Yuen MF, Chen DS, Dusheiko GM, Janssen HLA, Lau DTY, Locarnini SA, et al. Hepatitis B virus infection. Nature Reviews Disease Primers. 2018;4(1). https://doi.org/10.1038/nrdp.2018.35
  24. Yang Y, Sun JW, Zhao LG, Bray F, Xiang YB. Quantitative evaluation of hepatitis B virus mutations and hepatocellular carcinoma risk: a meta-analysis of prospective studies. Chinese J Cancer Research. 2015;27(5):497-508.  https://doi.org/10.3978/j.issn.1000-9604.2015.10.05
  25. Ge Z, Tian T, Meng L, Song C, Yu C, Xu X, et al. HBV mutations in EnhII/BCP/PC region contribute to the prognosis of hepatocellular carcinoma. Cancer Medicine. 2019;8(6):3086-3093. https://doi.org/10.1002/cam4.2169
  26. Gao J, Zuo R, Wang J, Shen T. Characteristics and evolutionary history of hepatitis B virus quasi-subgenotype B3 in Southeast Asia. Virus Research. 2019;273:197762. https://doi.org/10.1016/j.virusres.2019.197762
  27. Purdy MA, Talekar G, Swenson P, Araujo A, Fields H. A new algorithm for deduction of hepatitis B surface antigen subtype determinants from the amino acid sequence. Intervirology. 2007;50(1):45-51.  https://doi.org/10.1159/000096312
  28. Sleptsova SS. A role of the genotypes of hepatitis B, C and D viruses in primary liver cancer progression. VICh-infekcija i immunosupressii. 2012;4(4):67-73. (In Russ.).
  29. Kozhanova TV, Isaeva OV, Klushkina VV, Ourzhak ND, Sayan RM, Alekseeva MN, et al. Primary hepatitis B viral drug resistance to nucleos(t)ides analogues in hepatitis B viral-infected Patients. Dal’nevostochnyj zhurnal infekcionnoj patologii. 2011;18(18):41-47. (In Russ.).
  30. Chiou HL, Lee TS, Kuo J, Mau YC, Ho MS. Altered antigenicity of ‘a’ determinant variants of hepatitis B virus. J Gen Virology. 1997;78(10):2639-2645. https://doi.org/10.1099/0022-1317-78-10-2639
  31. Wu X, Zhou C, Gu WJ, Li HM, Qi ZB. Preparation of the national reference panel for hepatitis B surface antigen. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2008;22(4):311-313. (In Chinese).
  32. Grabarczyk P, Garmiri P, Liszewski G, Doucet D, Sulkowska E, Brojer E, et al. Molecular and serological characterization of hepatitis B virus genotype A and D infected blood donors in Poland. Journal of Viral Hepatitis. 2010;17(6):444-452.  https://doi.org/10.1111/j.1365-2893.2009.01192.x
  33. Heidrich B, Serrano B, Idilman R, Kabacam G, Bremer B, Raupach R, et al. HBeAg-positive hepatitis delta: virological patterns and clinical long-term outcome. Liver International. 2012;32(9):1415-1425. https://doi.org/10.1111/j.1478-3231.2012.02831.x
  34. Guedj J, Rotman Y, Cotler SJ, Koh C, Schmid P, Albrecht J, et al. Understanding early serum hepatitis D virus and hepatitis B surface antigen kinetics during pegylated interferon-alpha therapy via mathematical modeling. Hepatology. 2014;60(6):1902-1910. https://doi.org/10.1002/hep.27357

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