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Chub E.V.

State Research Center of Virology and Biotechnology «Vector», 630559, Koltsovo, Russia

Sivolobova G.F.

State Research Center of Virology and Biotechnology «Vector», 630559, Koltsovo, Russia

Netesov S.V.

Novosibirsk State University, Novosibirsk, Russia, 630090

Kochneva G.V.

State Research Center of Virology and Biotechnology «Vector», 630559, Koltsovo, Russia;
Novosibirsk state university, 630090, Novosibirsk, Russia

Recombinant variants of HCV in Siberia, Russia

Authors:

Chub E.V., Sivolobova G.F., Netesov S.V., Kochneva G.V.

More about the authors

Journal: Molecular Genetics, Microbiology and Virology. 2019;37(2): 64‑75

DOI: 10.17116/molgen20193702164

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To cite this article:

Chub EV, Sivolobova GF, Netesov SV, Kochneva GV. Recombinant variants of HCV in Siberia, Russia. Molecular Genetics, Microbiology and Virology. 2019;37(2):64‑75. (In Russ.)
https://doi.org/10.17116/molgen20193702164

 
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Abstract The review summarizes the results of a comprehensive multi-year molecular-epidemiological study of HCV (2001—2016) which started in Altai and Novosibirsk Regions when first eight cases of infection with the recombinant form of CRF01_1b2k HCV were detected. For all CRF01_1b2k isolates, the recombination site in the NS2 gene has been confirmed, that consistent with data for previously identified recombinants of the same form in other regions of Russia and the world. Analysis of the nucleotide sequences of the Core, E1, NS2 and NS5b gene fragments in genomes of eight recombinant 2k/1b isolates found in Siberia showed a close phylogenetic relationship between them, as well as 27 recombinants described to date in St. Petersburg, Azerbaijan, Armenia, Estonia, The Netherlands, Ireland, France and the United States. The level of homology between the genes of the HCV was as follows: for the Core gene — 97%, E1 — 94%, NS2 — 92%, NS5b — 96%. Phylogenetic analysis of genomes of recombinants showed the common origin of isolates of type 2k/1b and their wide circulation in the territory of Russia. Analysis of the 5’ part of the genome of recombinant isolates belonging to the 2k subtype showed that the closest isolates of the 2k subtype earlier, except for Russia, were also found in Moldova and Uzbekistan in 1996—2013. The incidence of recombinant isolates in the Siberian region is estimated at 1%. The molecular clock method has shown that the most probable time for the appearance of the recombinant form CRF01_1b2k is in the interval 1957—1970.

Keywords:

review
HCV
recombinant
form CRF01_1b2k
phylogenetic analysis
recombination point

Authors:

Chub E.V.

State Research Center of Virology and Biotechnology «Vector», 630559, Koltsovo, Russia

Sivolobova G.F.

State Research Center of Virology and Biotechnology «Vector», 630559, Koltsovo, Russia

Netesov S.V.

Novosibirsk State University, Novosibirsk, Russia, 630090

Kochneva G.V.

State Research Center of Virology and Biotechnology «Vector», 630559, Koltsovo, Russia;
Novosibirsk state university, 630090, Novosibirsk, Russia

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