Application of native platelet-rich autoplasma (PA) as a promoter of wound healing and reconstruction of the skin and soft tissue is becoming quite popular in various areas of clinical practice in view of its high safety and autologous origin. These effects are due to platelet-derived growth factors that infiltrate the skin as a result of degranulation of α-granules. Platelet-rich fibrin substrates are also used to accelerate tissue regeneration, but their use is limited since they are difficult to produce and expensive. The use of heat-treated fibrinous modification of PA is a simple and inexpensive method to produce fibrin substrate by heating platelet-rich autoplasma in an oven to the temperature of 56 °C. The use of native PA in combination with its heat-treated fibrinous modification enhances clinical effect of correction of age-related skin changes. Objective — the study was aimed at assessing structural changes in the skin after application of native platelet-rich autoplasma (PA) in combination with its heat-treated fibrinous form for correction of age-related changes. Comparing the dynamics of ultrasound scans of the skin in 20 patients before and after correction of age-related changes in the facial skin using native PA in combination with its fibrinous heat-treated modification. Material and methods. An open comparative prospective study involved 20 patients aged 32 to 63 years with age-related skin changes. We analyzed the results of ultrasound examination of the skin before and after treatment with native PA in combination with its heat-treated fibrinous modification. All patients underwent four procedures of application of native PA in combination with its fibrinous heat-treated modification once per week. Ultrasound study of the skin was carried out on the SonoScape SSI-8000-Pro instrument. Results. Ultrasound study showed that correction of age-related skin changes with native platelet-rich autoplasma (PA) in combination with its heat-treated fibrinous form increased epidermal thickness from 0.523±0.149 to 0.705±0.140 (which is highly significant, p<0.001). The thickness of the dermis as assessed by ultrasound examination increased from 1.391±0.316 to 1.509±0.334 (p=0.019). There were no significant changes in the thickness of subcutaneous fat (SF): 2.5±0.910 before correction, 2.55±0.468 after correction (p=0.559). After treatment, there was increase in point hyperechoic inclusions at the boundary of the epidermis and dermis, clear boundary line between the epidermis and dermis and increase in its unevenness, increase in dermal echogenicity due to increase in hyperechoic linear structures. Conclusions. These results suggest that the use of native PA in combination with its heat-treated fibrinous modification for correction of age-related skin changes causes structural changes in the skin: thickening of the epidermis and dermis and densening of the dermis. The absence of complications during the follow-up is indicative of high level of safety of this treatment.