Comparative evaluation of analytical sensitivity of microscopic methods (wet mount and fixed) and nucleic acid amplification assays (real time PCR and NASBA) for the detection of Trichomonas vaginalis

Ryzhikh P.G.

Federal state unitary enterprise "Central Research Institute of Epidemiology", Russian Federal Consumer Rights Protection and Human Health Control Service (Rospotrebnadzor)

Gushchin A.E.

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Welfare, Moscow, Russia

ORCID: 0000-0002-0399-1167

Savochkina Iu.A.

Federal state unitary enterprise "Central Research Institute of Epidemiology", Russian Federal Consumer Rights Protection and Human Health Control Service (Rospotrebnadzor)

Comparative evaluation of analytical sensitivity of microscopic methods (wet mount and fixed) and nucleic acid amplification assays (real time PCR and NASBA) for the detection of Trichomonas vaginalis

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Ryzhikh P.G., Gushchin A.E., Savochkina Iu.A.

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Journal: Russian Journal of Clinical Dermatology and Venereology. 2011;9(2): 28‑35

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Ryzhikh PG, Gushchin AE, Savochkina IuA. Comparative evaluation of analytical sensitivity of microscopic methods (wet mount and fixed) and nucleic acid amplification assays (real time PCR and NASBA) for the detection of Trichomonas vaginalis. Russian Journal of Clinical Dermatology and Venereology. 2011;9(2):28‑35. (In Russ.)

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There are three principal methods currently used in our country and worldwide to detect Trichomonas vaginalis, viz. microscopic study of native and stained preparations, culturing of microorganisms, and identification of their nucleic acids by the nucleic acid amplification (NAA) techniques. The objective of the present study was to directly compare detection limits for T. vaginalis using microscopy of native or stained preparations and AmpliSens commercial assay kits (manufactured by the Central Research Institute of Epidemiology) for real time PCR and NASBA. It was shown that detection limits of real time PCR and NASBA are 100-1000 lower than those of the microscopic techniques. It suggests a higher analytical sensitivity of NAA assays.

Keywords:

T. vaginalis

PCR

NASBA

microscopy

Authors:

Ryzhikh P.G.

Federal state unitary enterprise "Central Research Institute of Epidemiology", Russian Federal Consumer Rights Protection and Human Health Control Service (Rospotrebnadzor)

Gushchin A.E.

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Welfare, Moscow, Russia

ORCID: 0000-0002-0399-1167

Savochkina Iu.A.

Federal state unitary enterprise "Central Research Institute of Epidemiology", Russian Federal Consumer Rights Protection and Human Health Control Service (Rospotrebnadzor)

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