Comparative evaluation of analytical sensitivity of microscopic methods (wet mount and fixed) and nucleic acid amplification assays (real time PCR and NASBA) for the detection of Trichomonas vaginalis

Ryzhikh P.G.; Gushchin A.E.; Savochkina Iu.A.

doi:https://doi.org/

Ryzhikh P.G.

Federal state unitary enterprise "Central Research Institute of Epidemiology", Russian Federal Consumer Rights Protection and Human Health Control Service (Rospotrebnadzor)

Gushchin A.E.

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Welfare, Moscow, Russia

ORCID: 0000-0002-0399-1167

Savochkina Iu.A.

Federal state unitary enterprise "Central Research Institute of Epidemiology", Russian Federal Consumer Rights Protection and Human Health Control Service (Rospotrebnadzor)

Comparative evaluation of analytical sensitivity of microscopic methods (wet mount and fixed) and nucleic acid amplification assays (real time PCR and NASBA) for the detection of Trichomonas vaginalis

Authors:

Ryzhikh P.G., Gushchin A.E., Savochkina Iu.A.

More about the authors

Journal: Russian Journal of Clinical Dermatology and Venereology. 2011;9(2): 28‑35

Read: 3265 times

Download PDF (RU)

Contact the author

Table of contents

To cite this article:

Ryzhikh PG, Gushchin AE, Savochkina IuA. Comparative evaluation of analytical sensitivity of microscopic methods (wet mount and fixed) and nucleic acid amplification assays (real time PCR and NASBA) for the detection of Trichomonas vaginalis. Russian Journal of Clinical Dermatology and Venereology. 2011;9(2):28‑35. (In Russ.)

Подписка 2026 Клиническая дерматология и венерология (Russian Journal of Clinical Dermatology and Venereology)

Использование инфографики авторами научных работ

Close metadata

Recommended articles:

Trichomoniasis. Concentration of Pathogen DNA and Its Relationship with Clinical Manifestations of the Disease. Russian Journal of Clinical Dermatology and Venereology. 2024;(6):656-661

M. genitalium-infection. Correlation between pathogen’s DNA concentration and clinical picture of disease. Russian Journal of Clinical Dermatology and Venereology. 2025;(4):446-452

Integration of NGS testing with conventional molecular genetic methods in oncology. P.A. Herzen Journal of Oncology. 2024;(6):84-90

Laboratory diagnostics of monkeypox: basic methods and prospects for new developments. Laboratory Service. 2024;(4):57-64

Neoadjuvant drug therapy and oncological outcomes in patients with stage I–II breast cancer. P.A. Herzen Journal of Oncology. 2025;(1):5-12

Treatment outcomes of stage IIA–IIB breast cancer patients of hormone-positive HER2-negative subtype after neoadjuvant polychemotherapy depending on Ki-67 levels. P.A. Herzen Journal of Oncology. 2025;(2):13-18

Abstract:

There are three principal methods currently used in our country and worldwide to detect Trichomonas vaginalis, viz. microscopic study of native and stained preparations, culturing of microorganisms, and identification of their nucleic acids by the nucleic acid amplification (NAA) techniques. The objective of the present study was to directly compare detection limits for T. vaginalis using microscopy of native or stained preparations and AmpliSens commercial assay kits (manufactured by the Central Research Institute of Epidemiology) for real time PCR and NASBA. It was shown that detection limits of real time PCR and NASBA are 100-1000 lower than those of the microscopic techniques. It suggests a higher analytical sensitivity of NAA assays.

Keywords:

T. vaginalis

PCR

NASBA

microscopy

Authors:

Ryzhikh P.G.

Federal state unitary enterprise "Central Research Institute of Epidemiology", Russian Federal Consumer Rights Protection and Human Health Control Service (Rospotrebnadzor)

Gushchin A.E.

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Welfare, Moscow, Russia

ORCID: 0000-0002-0399-1167

Savochkina Iu.A.

Federal state unitary enterprise "Central Research Institute of Epidemiology", Russian Federal Consumer Rights Protection and Human Health Control Service (Rospotrebnadzor)

Close metadata

References:

Prikaz Minzdrava SSSR ot 4 dekabrya 1986 g. №1570 "Ob uluchshenii vyyavleniya bol'nykh gonoreei i trikhomoniazom v akusherskikh i ginekologicheskikh otdeleniyakh (palatakh, kabinetakh), zhenskikh konsul'tatsiyakh i urologicheskikh kabinetakh poliklinik". M 1986.
Prikaz Minzdrava RF ot 23.12.02 g. №398 "Ob utverzhdenii perechnya prikazov Minzdrava SSSR, priznannykh ne deistvuyushchimi na territorii Rossiiskoi Federatsii, po razdelu "Okhrana materinstva i detstva". M 2002.
Ryu J.S., Chung H.L., Min D.Y. et al. Diagnosis of Trichomoniasis by Polymerase Chain Reaction. Yonsei Med J 1999;40:1:56-60.
Schwebke J.R., Lawing L.F. Improved detection by DNA amplification of Trichomonas vaginalis in Males. J Clin Microbiol 2002;40:10:3681-3683.
Patel S.R., Wiese W., Patel S.C. et al. Systematic review of diagnostic tests for vaginal trichomoniasis. Infect Dis Obstet Gynecol 2000;8:5-6:248-257.
Simeshchenko I.E., Mikhailov N.V., Lavrent'ev I.A. Rezul'taty obnaruzheniya trikhomonad kul'tural'nym metodom i polimeraznoi tsepnoi reaktsiei. V kn.: Genodiagnostika infektsionnykh boleznei. Vserossiiskaya nauchno-prakticheskaya konferentsiya, 5-ya: Trudy. M: Meditsina 2004;1:117-118.
Mavzyutov A.R., Mustafina G.R., Nikonorov Yu.M., Khismatulina Z.R. Sravnitel'naya otsenka informativnosti metodov laboratornoi diagnostiki trikhomoniaza u muzhchin. Ros zhurn kozhn i ven bol 2010;5:51-54.
Crucitti T., Van Dyck E., Tehe A. et al. Comparison of culture and different PCR assays for detection of Trichomonas vaginalis in self collected vaginal swab specimens. Sex Transm Infect 2003;79:5:393-398.
Sokolovskii E.V., Kisina V.I., Savicheva A.M. i dr. Klinicheskaya interpretatsiya rezul'tatov mikroskopicheskogo metoda diagnostiki urogenital'nykh infektsii: rekomendatsii dlya vrachei. St-Peterburg 2010;88.
Frigo N.V., Rotanov S.V., Lesnaya I.N. i dr. Laboratornaya diagnostika IPPP v Rossiiskoi Federatsii. Rezul'taty natsional'nogo issledovaniya. Vestn dermatol i venerol 2008;5:33-41.
Paces J., Urbánková V., Urbánek P. Cloning and characterization of a repetitive DNA sequence specific for Trichomonas vaginalis. Mol Biochem Parasitol 1992;54:2:247-255.