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Beloglazova I.B.

Chazov National Medical Research Center of Cardiology named after academician E.I. Chazov

Zubkova E.S.

Chazov National Medical Research Center of Cardiology named after academician E.I. Chazov

Dergilev K.V.

Chazov National Medical Research Center of Cardiology named after academician E.I. Chazov

Ratner E.I.

Chazov National Medical Research Center of Cardiology named after academician E.I. Chazov

Guseva A.A.

Chazov National Medical Research Center of Cardiology named after academician E.I. Chazov

Menshikov M.Yu.

Chazov National Medical Research Center of Cardiology named after academician E.I. Chazov

Parfenova Ye.V.

Chazov National Medical Research Center of Cardiology named after academician E.I. Chazov;
Lomonosov Moscow State University

VEGF in regulation of non-canonical Notch signaling pathway in endothelial cells

Authors:

Beloglazova I.B., Zubkova E.S., Dergilev K.V., Ratner E.I., Guseva A.A., Menshikov M.Yu., Parfenova Ye.V.

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Journal: Russian Cardiology Bulletin. 2022;17(2): 33‑39

DOI: 10.17116/Cardiobulletin20221702133

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Table of contents

VEGF IN REGULATION OF NON-CANONICAL NOTCH SIGNALING PATHWAY IN ENDOTHELIAL CELLS
VEGF IN REGULATION OF NON-CANONICAL NOTCH SIGNALING PATHWAY IN ENDOTHELIAL CELLS

To cite this article:

Beloglazova IB, Zubkova ES, Dergilev KV, Ratner EI, Guseva AA, Menshikov MYu, Parfenova YeV. VEGF in regulation of non-canonical Notch signaling pathway in endothelial cells. Russian Cardiology Bulletin. 2022;17(2):33‑39. (In Russ.)
https://doi.org/10.17116/Cardiobulletin20221702133

Подписка 2025-2 (Russian Cardiology Bulletin)
 
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OBJECTIVE

To investigate the possibility of crosstalk between VEGF and non-canonical Notch signaling pathways.

MATERIAL AND METHODS

We evaluated the influence of VEGF and Notch on ability of umbilical vein endothelial cells (HUVEC) to form vascular sprouts in 3D fibrin gel in vitro angiogenesis assay.

RESULTS

We found that VEGF inhibitor (DMH4) significantly reduced the number and length of endothelial sprouts. Notch signaling inhibitors reduced sprout formation to a lesser extent. Analysis of phospho-kinase proteomic profile of endothelial cells after addition of VEGF and/or Jagged1 (Notch ligand) showed that VEGF enhances CREB transcription factor phosphorylation, while Jagged1 activates such components of non-canonical Notch signaling as HCK, enhances expression of Hsp60 and b-catenin, and suppresses phosphorylation of p70S6 kinase. The combined action of VEGF and Jagged1 leads to enhanced HCK activation and suppression of p70S6.

Keywords:

VEGF
Notch
HCK
p70S6
Hsp60
CREB
b-catenin
endothelium
3D model of angiogenesis

Authors:

Beloglazova I.B.

Chazov National Medical Research Center of Cardiology named after academician E.I. Chazov

Zubkova E.S.

Chazov National Medical Research Center of Cardiology named after academician E.I. Chazov

Dergilev K.V.

Chazov National Medical Research Center of Cardiology named after academician E.I. Chazov

Ratner E.I.

Chazov National Medical Research Center of Cardiology named after academician E.I. Chazov

Guseva A.A.

Chazov National Medical Research Center of Cardiology named after academician E.I. Chazov

Menshikov M.Yu.

Chazov National Medical Research Center of Cardiology named after academician E.I. Chazov

Parfenova Ye.V.

Chazov National Medical Research Center of Cardiology named after academician E.I. Chazov;
Lomonosov Moscow State University

Received:

21.03.2022

Accepted:

11.04.2022

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