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Senokosova E.A.

Research Institute for Complex Issues of Cardiovascular Diseases

Krutitskii S.S.

Research Institute for Complex Issues of Cardiovascular Diseases

Krivkina O.E.

Research Institute for Complex Issues of Cardiovascular Diseases

Evtushenko V.V.

Research Cardiology Institute, Tomsk National Research Medical Center

Evtushenko A.V.

Research Institute for Complex Issues of Cardiovascular Diseases

Antonova L.V.

Research Institute for Complex Issues of Cardiovascular Diseases

Grigoriyev E.V.

Research Institute for Complex Issues of Cardiovascular Diseases

Verification of a new method for intraoperative myocardium monitoring

Authors:

Senokosova E.A., Krutitskii S.S., Krivkina O.E., Evtushenko V.V., Evtushenko A.V., Antonova L.V., Grigoriyev E.V.

More about the authors

Journal: Russian Journal of Anesthesiology and Reanimatology. 2021;(5): 29‑33

DOI: 10.17116/anaesthesiology202105129

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Table of contents

VERIFICATION OF A NEW METHOD FOR INTRAOPERATIVE MYOCARDIUM MONITORING
VERIFICATION OF A NEW METHOD FOR INTRAOPERATIVE MYOCARDIUM MONITORING

To cite this article:

Senokosova EA, Krutitskii SS, Krivkina OE, Evtushenko VV, Evtushenko AV, Antonova LV, Grigoriyev EV. Verification of a new method for intraoperative myocardium monitoring. Russian Journal of Anesthesiology and Reanimatology. 2021;(5):29‑33. (In Russ.)
https://doi.org/10.17116/anaesthesiology202105129

МСФ на ЯМ
Использование инфографики авторами научных работ
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Abstract:

OBJECTIVE

To compare the efficacy of non-contact intraoperative optical myocardial biopsy in situ based on laser-induced fluorescence and immunohistochemical/histological examination.

MATERIAL AND METHODS

The study included patients undergoing on-pump cardiac surgery (n=15). Intraoperative online heart monitoring was performed using laser-induced fluorescence (λ=365 nm). We analyzed collagen and elastin fluorescence coefficients. Integral fluorescence index was intraoperatively recorded at 4 time points: 1 — before aortic occlusion; 2 — after aortic occlusion and cardioplegia; 3 — before aortic clamp removal; 4 — after weaning from heart-lung machine. We compared the results of heart monitoring, immunohistochemical staining (n=42) (metalloproteinase-3 (MMP-3) and inhibitor of matrix metalloproteinases-1(TIMP-1)) and Van Gieson staining of myocardial tissue specimens.

RESULTS

Elastin fluorescence was significantly more intense only in the area of intact myocardium at the beginning of aortic occlusion compared to previous surgical stage (p<0.05). There were no significant differences in collagen fluorescence. MMP-3 and its inhibitor TIMP-1 did not accumulate in intercellular space. Van Gieson staining confirmed structural integrity of myocardial tissue. Consistency of these results demonstrates efficiency and high sensitivity of laser-induced fluorescence method.

CONCLUSION

Laser-induced fluorescence is highly informative, simple for use and interpretation method that can become a perspective approach in cardiac surgery.

Keywords:

Keyword: laser-induced fluorometry
extracellular matrix
cardiac surgery
monitoring
cardiopulmonary bypass

Authors:

Senokosova E.A.

Research Institute for Complex Issues of Cardiovascular Diseases

Krutitskii S.S.

Research Institute for Complex Issues of Cardiovascular Diseases

Krivkina O.E.

Research Institute for Complex Issues of Cardiovascular Diseases

Evtushenko V.V.

Research Cardiology Institute, Tomsk National Research Medical Center

Evtushenko A.V.

Research Institute for Complex Issues of Cardiovascular Diseases

Antonova L.V.

Research Institute for Complex Issues of Cardiovascular Diseases

Grigoriyev E.V.

Research Institute for Complex Issues of Cardiovascular Diseases

Received:

15.03.2021

Accepted:

17.04.2021

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References:

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