Trichomoniasis. Concentration of Pathogen DNA and Its Relationship with Clinical Manifestations of the Disease

E.S. Negasheva

Moscow Scientific and Practical Center of Dermatovenereology and Cosmetology

ORCID: 0000-0001-5613-6482

N.V. Frigo

Moscow Scientific and Practical Center of Dermatovenereology and Cosmetology;
State Research Center — Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency

ORCID: 0000-0001-6231-971X

A.E. Gushchin

Moscow Scientific and Practical Center of Dermatovenereology and Cosmetology

ORCID: 0000-0002-0399-1167

O.V. Dolya

Moscow Scientific and Practical Center of Dermatovenereology and Cosmetology;
Patrice Lumumba Peoples’ Friendship University of Russia

ORCID: 0000-0001-5019-4593

S.A. Polevshchikova

Moscow Scientific and Practical Center of Dermatovenereology and Cosmetology

ORCID: 0000-0002-8018-086X

Journal: Russian Journal of Clinical Dermatology and Venereology. 2024;23(6): 656–661

Trichomoniasis. Concentration of Pathogen DNA and Its Relationship with Clinical Manifestations of the Disease

Authors:

E.S. Negasheva, N.V. Frigo, A.E. Gushchin, O.V. Dolya, S.A. Polevshchikova

More about the authors

DOI: 10.17116/klinderma202423061656

Read: 2932 times

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Negasheva ES, Frigo NV, Gushchin AE, Dolya OV, Polevshchikova SA. Trichomoniasis. Concentration of Pathogen DNA and Its Relationship with Clinical Manifestations of the Disease. Russian Journal of Clinical Dermatology and Venereology. 2024;23(6):656‑661. (In Russ., In Engl.).
https://doi.org/10.17116/klinderma202423061656

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RELEVANCE

Trichomoniasis holds a leading position among sexually transmitted infections in Russia. However, there is currently a lack of literature on the results of quantitative analysis of the protozoal load of the pathogen in both men and women.

OBJECTIVE

To investigate the level of protozoal load (DNA concentration) of T. vaginalis in patients with trichomoniasis and its relationship with the clinical course of the disease.

MATERIAL AND METHODS

Study subjects: 82 patients with trichomoniasis. Gender composition: 10 men and 72 women. Age range: patients aged from 17 to 73 years. Research material: urogenital scraping. Method of study: multiplex real-time PCR with quantitative determination of T. vaginalis DNA.

RESULTS

Patients with a low protozoal load of T. vaginalis predominated in the group of trichomoniasis patients (78.0%), including both men (60%) and women (81%). The severity of clinical symptoms was found to correlate with the level of T. vaginalis protozoal load, with a statistically significant direct relationship observed in the overall group of patients (χ²=106.85; OR 42.48; p<0.001) among women (χ²=135.13; OR 451; p<0.001), and among men (χ²=63.19; OR 99; p<0.001).

CONCLUSION

The level of T. vaginalis DNA concentration/protozoal load in the urogenital tract of patients influences the severity of trichomoniasis symptoms. Quantitative determination of T. vaginalis protozoal load should be considered during initial diagnosis and when prescribing etiological treatment for trichomoniasis.

Keywords:

Trichomonas vaginalis

Authors:

E.S. Negasheva

Moscow Scientific and Practical Center of Dermatovenereology and Cosmetology

ORCID: 0000-0001-5613-6482

N.V. Frigo

Moscow Scientific and Practical Center of Dermatovenereology and Cosmetology;
State Research Center — Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency

ORCID: 0000-0001-6231-971X

A.E. Gushchin

Moscow Scientific and Practical Center of Dermatovenereology and Cosmetology

ORCID: 0000-0002-0399-1167

O.V. Dolya

Moscow Scientific and Practical Center of Dermatovenereology and Cosmetology;
Patrice Lumumba Peoples’ Friendship University of Russia

ORCID: 0000-0001-5019-4593

S.A. Polevshchikova

Moscow Scientific and Practical Center of Dermatovenereology and Cosmetology

ORCID: 0000-0002-8018-086X

Received:

17.05.2024

Accepted:

22.09.2024

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