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E.A. Starikova

Institute of Experimental Medicine;
Pavlov First Saint Petersburg State Medical University;
Institute of Medical Education, Almazov National Medical Research Center

J.T. Mammedova

Institute of Experimental Medicine

A.V. Sokolov

Institute of Experimental Medicine

N.A. Podoplelova

Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology

A.B. Malashicheva

Almazov National Medical Research Center;
Cytology Institute of the Russian Academy of Sciences

O.Ya. Porembskaya

Institute of Experimental Medicine;
Mechnikov North-Western State Medical University

V.A. Tsinzerling

Almazov National Medical Research Center

N.V. Solovyev

Marimix LLC

Journal: Journal of Venous Disorders. 2023;17(4): 302–311

Antithrombotic Effects of Blue Mussel Acid Hydrolysate

Authors:

E.A. Starikova, J.T. Mammedova, A.V. Sokolov, N.A. Podoplelova, A.B. Malashicheva, O.Ya. Porembskaya, V.A. Tsinzerling, N.V. Solovyev

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DOI: 10.17116/flebo202317041302

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Starikova EA, Mammedova JT, Sokolov AV, Podoplelova NA, Malashicheva AB, Porembskaya OYa, Tsinzerling VA, Solovyev NV. Antithrombotic Effects of Blue Mussel Acid Hydrolysate. Journal of Venous Disorders. 2023;17(4):302‑311. (In Russ., In Engl.).
https://doi.org/10.17116/flebo202317041302

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Thrombosis is a venous or arterial vascular that can sometimes cause fatal outcomes. Development of effective pharmacological agents for antithrombotic therapy is still an urgent scientific and practical objective.

OBJECTIVE

To study the antithrombotic effects of blue mussel (Mytilus edulis) hydrolysate preparation.

MATERIAL AND METHODS

Biochemical methods were used to analyze the effect of blue mussel hydrolysate on enzymatic activity of key serine proteases in clotting cascade. We assessed the structure of actin cytoskeleton and endothelial monolayer morphology in vitro to investigate the effect of this drug on biological activity of thrombin. Anticoagulant effect of hydrolysate was investigated in a model of posterior vena cava thrombosis in rats.

RESULTS

The drug inhibits serine proteinases of clotting cascade (FIIa, FXa, FXIa, FXIIa), in particular, enzymatic activity of thrombin (FIIa) against fibrinogen and synthetic substrates Tos-Gly-Pro-Arg-pNA and Ala-Ala-Arg-pNA. Anti-thrombin activity of undiluted drug was about 6.5 AT-U/ml. Hydrolysate inhibited the ability of thrombin to cause actin cytoskeleton remodeling and endothelial monolayer disintegration. Subcutaneous injections significantly changed thrombus structure and reduced the probability of clotting in a model of posterior vena cava thrombosis in rats.

CONCLUSION

M. edulis hydrolysate may be a basis for development of new drugs for therapy of hypercoagulable conditions.

Keywords:

M.edulis hydrolysate
venous thrombosis
antithrombotic therapy
thrombin
endothelial cells
anticoagulation

Authors:

E.A. Starikova

Institute of Experimental Medicine;
Pavlov First Saint Petersburg State Medical University;
Institute of Medical Education, Almazov National Medical Research Center

J.T. Mammedova

Institute of Experimental Medicine

A.V. Sokolov

Institute of Experimental Medicine

N.A. Podoplelova

Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology

A.B. Malashicheva

Almazov National Medical Research Center;
Cytology Institute of the Russian Academy of Sciences

O.Ya. Porembskaya

Institute of Experimental Medicine;
Mechnikov North-Western State Medical University

V.A. Tsinzerling

Almazov National Medical Research Center

N.V. Solovyev

Marimix LLC

Received:

03.10.2023

Accepted:

14.10.2023

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