The site of the Media Sphera Publishers contains materials intended solely for healthcare professionals.
By closing this message, you confirm that you are a certified medical professional or a student of a medical educational institution.

Login
EN
+7 (495) 482-4118
+7 (495) 482-4329
+8 800 250-18-12
  • (toll-free number for subscriptions)
    Mon-Fri from 10 a.m. to 6 p.m.

  • © 1998-2025
+7 (495) 482-43-29
EN
All journals
Journal
Year
Year
Search result: 0

Gar’kavenko V.V.

Krasnoyarsk Regional Ophthalmological Clinical Hospital named after Professor P.G. Makarov

Salmin V.V.

National Research Nuclear University «MEPhI» (Moscow Engineering Physics Institute);
Moscow Institute of Physics and Technology (National Research University);
Moscow State Technical University named after N.E. Bauman (National Research University)

Balashova P.M.

Krasnoyarsk Regional Ophthalmological Clinical Hospital named after Professor P.G. Makarov;
Krasnoyarsk State Medical University named after Professor V.F. Voino-Yasenetsky

Gaydelis V.S.

Krasnoyarsk Regional Ophthalmological Clinical Hospital named after Professor P.G. Makarov

Kokozova D.S.

Krasnoyarsk Regional Ophthalmological Clinical Hospital named after Professor P.G. Makarov

Spectrofluorimetric changes in the lens after intravitreal injections of brolucizumab

Authors:

Gar’kavenko V.V., Salmin V.V., Balashova P.M., Gaydelis V.S., Kokozova D.S.

More about the authors

Journal: Russian Annals of Ophthalmology. 2023;139(6): 41‑49

DOI: 10.17116/oftalma202313906141

Read: 1394 times

Download PDF (RU)
Contact the author
Table of contents

SPECTROFLUORIMETRIC CHANGES IN THE LENS AFTER INTRAVITREAL INJECTIONS OF BROLUCIZUMAB
SPECTROFLUORIMETRIC CHANGES IN THE LENS AFTER INTRAVITREAL INJECTIONS OF BROLUCIZUMAB

To cite this article:

Gar’kavenko VV, Salmin VV, Balashova PM, Gaydelis VS, Kokozova DS. Spectrofluorimetric changes in the lens after intravitreal injections of brolucizumab. Russian Annals of Ophthalmology. 2023;139(6):41‑49. (In Russ.)
https://doi.org/10.17116/oftalma202313906141

Подписка 2026 Вестник офтальмологии (Russian Annals of Ophthalmology)
МСФ на ЯМ
Использование инфографики авторами научных работ
Close metadata
Recommended articles:
Mana­gement of patients with treatment-resistant neovascular age-related macu­lar dege­neration. Russian Annals of Ophthalmology. 2024;(6):63-68
Choroidal neovascularization asso­ciated with choroidal nevi. Russian Annals of Ophthalmology. 2025;(1):104-112
Abstract:

Intravitreal injections (IVI) of vascular endothelial growth factor (VEGF) inhibitors are actively used in the treatment of various ophthalmic pathologies. In addition to the pronounced therapeutic effect of anti-VEGF drugs described in the literature, a number of data on adverse effects associated with the use of IVI, including from the lens, have now been accumulated. Prevention of possible side effects of this type of treatment requires further investigation.

PURPOSE

The study evaluates the changes in spectrofluorometric parameters of the lens after intravitreal injections of brolucizumab in eyes with native lens.

MATERIAL AND METHODS

The study included 13 people with neovascular age-related macular degeneration (AMD) who received IVI of brolucizumab. All patients were over 40 years old; changes in central retinal thickness in the macular area exceeded 300 μm according to optical coherence tomography. Spectrofluorimetric indicators of the lens were studied before and after injections of the drug in both eyes. The spectra were analyzed in the wavelength range 412—698 nm to record the changes in the content of non-tryptophan fluorophore molecules in the lens.

RESULTS

Spectrofluorimetry revealed metabolic changes in the native lens after intravitreal injections of the drug brolucizumab. No such changes were observed in the lens of the eyes not injected with the drug (the «Control» group). The proposed investigation technique involves prospective assessment of the safety of various molecules injected intravitreally in terms of cataract development at various times after the injection.

CONCLUSION

Spectrofluorimetry can be used for identifying initial metabolic changes in the lens. It is a promising method for assessing the molecules used in intravitreal injections in terms of their safety for the native lens, as well as for development of detailed instructions for clinically substantiated use of drugs with adverse effects in the form of cataract induction. According to spectrofluorimetry, IVI of the drug brolucizumab affects metabolic changes in the lens.

Keywords:

antiangiogenic therapy
lens
spectrofluorimetry

Authors:

Gar’kavenko V.V.

Krasnoyarsk Regional Ophthalmological Clinical Hospital named after Professor P.G. Makarov

Salmin V.V.

National Research Nuclear University «MEPhI» (Moscow Engineering Physics Institute);
Moscow Institute of Physics and Technology (National Research University);
Moscow State Technical University named after N.E. Bauman (National Research University)

Balashova P.M.

Krasnoyarsk Regional Ophthalmological Clinical Hospital named after Professor P.G. Makarov;
Krasnoyarsk State Medical University named after Professor V.F. Voino-Yasenetsky

Gaydelis V.S.

Krasnoyarsk Regional Ophthalmological Clinical Hospital named after Professor P.G. Makarov

Kokozova D.S.

Krasnoyarsk Regional Ophthalmological Clinical Hospital named after Professor P.G. Makarov

Received:

15.09.2023

Accepted:

14.11.2023

Close metadata

References:

  1. Steinmetz JD. Causes of blindness and vision impairment in 2020 and trends over 30 years, and prevalence of avoidable blindness in relation to VISION 2020: the Right to Sight: an analysis for the Global Burden of Disease Study. Lancet Global Health. 2021;9(2):e144-e160.
  2. Williams GA. IVT injections: health policy implications. Rev Ophthalmol. 2014;21:62-64. 
  3. Boyko EV, Sosnovsky SV, Berezin RD, Kacherovich PA, Tavtilova DA. Intravitreal injections: theory and practice. Ophthalmology Reports = Oftal’mologicheskie vedomosti. 2010;3(2):28-35. (In Russ.).
  4. Enoch J, Ghulakhszian A, Sekhon M, Crabb DP, Taylor DJ, Dinah C. Towards a Therapy for Geographic Atrophy: A Patient’s Experience. Patient Prefer Adher. 2023;17:299-310. 
  5. Rofagha S. Minimizing risks to patients by improving presentation of clinical trial results in geographic atrophy trials. Ophthalmol Retina. 2022;6(5): 337-338. 
  6. Khan H, Aziz AA, Sulahria H, Khan H, Ahmed A, Choudhry N, Narayanan R, Danzig C, Khanani AM Emerging Treatment Options for Geographic Atrophy (GA) Secondary to Age-Related Macular Degeneration. Clin Ophthalmol. 2023;17:321-327. 
  7. Wu AL, Wu WC. Anti-VEGF for ROP and Pediatric Retinal Diseases. Asia Pac J Ophthalmol (Phila). 2018;7(3):145-151.  https://doi.org/10.22608/APO.201837
  8. Sadovnikova NN, Prisich NV, Brzhesky VV. Anti-VEGF therapy for retinopathy of prematurity. Literature review. Russian Pediatric Ophthalmology = Rossiiskaya pediatricheskaya oftal’mologiya. 2016;11(4):212-220. (In Russ.). https://doi.org/10.18821/1993-1859-2016-11-4-212-220
  9. Abouammoh MA. Advances in the treatment of central serous chorioretinopathy. Saudi J Ophthalmol. 2015;29(4):278-286.  https://doi.org/10.1016/j.sjopt.2015.01.007
  10. Fung AE, Rosenfeld PJ, Reichel E. The International Bevacizumab safety Surrey: using the Internet to assess drug safety Worldwide. Br J Ophthalmol. 2006;90(11):1344-1349.
  11. Andrs-Guerrero V, Perucho-Gonzalez L, Garcia-Feijoo J. Current Perspectives on the Use of Anti-VEGF Drugs as Adjuvant Therapy in Glaucoma. Adv Ther. 2016;34(2):378-395.  https://doi.org/10.1007/s12325-016-0461-z
  12. Spaide RF, Chung JE, Fisher YL. Ultrasound detection of sili- cone oil after its removal in retinal reattachment surgery. Retina. 2005;25(7):943-945.  https://doi.org/10.1097/00006982-200510000-00022
  13. Stone TW, ed. ASRS 2018 Preferences and Trends Member- ship Survey. Chicago, IL. American Society of Retina Specialists; 2018. Accessed: 13.05.21.  https://www.asrs.org/content/documents/_2018-pat-survey-results-for-website.pdf
  14. Khurana RN, Chang LK, Porco TC. Incidence of presumed silicone oil droplets in the vitreous cavity after intravitreal beva- cizumab injection with insulin syringes. JAMA Ophthalmol. 2017;135(7):800-803.  https://doi.org/10.1001/jamaophthalmol.2017.1815
  15. Melo GB, Dias Jr CS, Morais FB, Cardoso AL, Figueiredo AGA, Filho AASL, et al. Prevalence of silicone oil droplets in eyes treated with intravitreal injection. Int J Retina Vitreous. 2019;5:34.  https://doi.org/10.1186/s40942-019-0184-9
  16. Movsisyan AB, Glazko NG, Brezhnev AYu, Kuroyedov AV. Influence of intravitreal drug administration on the course and prognosis of primary open-angle glaucoma. Clinical Ophthalmology = Klinicheskaya oftal’mologiya. 2021;21(4):227-234. (In Russ.). https://doi.org/10.32364/2311-7729-2021-21-4-227-234
  17. Af Segerstad PH. Risk model for intraoperative complication during cataract surgery based on data from 900 000 eyes: previous intravitreal injection is a risk factor. Brit J Ophthalmol. 2022;106(10):1373-1379.
  18. Leite J. Castro C, Abreu AC, Pessoa BT. Posterior capsular rupture during cataract surgery in eyes treated previously with intravitreal injections. Ophthalmologica. 2023;246(1):9-13.  https://doi.org/10.1159/000528657
  19. Jager RD, Aiello LP, Patel SC, Cunningham ET Jr. Risks of intravitreous injection: a comprehensive review. Retina. 2004;24(5):676-698. 
  20. Mannermaa E, Vellonen KS, Urtti A. Drug transport in corneal epithelium and blood retina barrier: emerging role of transporters in ocular pharmacokinetics. Adv Drug Deliv Rev. 2006;58:1136-1163.
  21. Lipatov DV, Skladchikov SA, Savenkova NP, Novoderezhkin VV, Vysikailo FI. Mathematical modeling of the movement of fluids inside the eyeball during intravitreal injections. Russian Ophthalmological Journal = Rossiiskii oftal’mologicheskii zhurnal. 2022;15(2):37-41. (In Russ.). https://doi.org/10.21516/2072-0076-2022-15-2-37-41
  22. Andreeva YuS, Alkharki L, Shelankova AV, Budzinskaya MV. Dynamic changes in the anterior chamber against the background of intravitreal injections of an anti-VEGF drug. Bulletin of Pirogov the National Medical and Surgical Center = Vestnik Natsional’nogo mediko-khirurgicheskogo Tsentra im. N.I. Pirogova. 2022;17(4):22-24. (In Russ.). https://doi.org/10.25881/20728255-2022-17-4-S1-22
  23. Siik S. Lens autofluorescence and light scatter in relation to the lens opacities classification system, LOCS III. Acta Ophthalmol Scand. 1999;77(5): 509-514. 
  24. Holló CT, Miháltz K, Kurucz M, Csorba A, Kránitz K, Kovács I, Nagy ZZ, Erdei G. Objective quantification and spatial mapping of cataract with a Shack-Hartmann wavefront sensor. Sci Rep. 2020;10(1):12585.
  25. De Castro A, Benito A, Manzanera S, Mompeán J, Cañizares B, Martínez D, Marín JM, Grulkowski I, Artal P. Three-dimensional cataract crystalline lens imaging with swept-source optical coherence tomography. Invest Ophthalmol Vis Sci. 2018;59(2):897-903. 
  26. Kiseleva TN, Zaitsev MS. Innovative technologies in age-related cataract monitoring. Ophthalmology = Oftal’mologiya. 2022;19(4):740-745. (In Russ.). https://doi.org/10.18008/1816-5095-2022-4-740-745
  27. Bullard B. Pyridine nucleotides in the lens of different species. Exper Eye Res. 1965;4(2):108-111. 
  28. Stewart RC. Augusteyn, Pyridine nucleotides in normal and cataractous human lenses. Expe Eye Res. 1984;39(3):307-315. 
  29. Azouaoui D, Choinière MR, Khan M, Sayfi S, Jaffer S, Yousef S, Patten DA, Green A, Menzies K J. Meta-analysis of NAD(P)(H) quantification results exhibits variability across mammalian tissues. Sci Rep. 2023;13(1):2464. https://doi.org/10.1038/s41598-023-29607-8
  30. Tsentalovich YuP, Snytnikova OA, Sagdeev RZ. Photochemical and thermal reactions of kynurenines. Russ Chem Rev. 2008;77(9):789-797.  https://doi.org/10.1070/RC2008v077n09ABEH003880
  31. Shapovalov KA, Salmin VV, Lazarenko VI, Gar′kavenko VV Modeling of the autofluorescence spectra of the crystalline lens with cataract taking into account light scattering. J Appl Spectroscopy. 2017;4(2):278-283.  https://doi.org/10.1007/s10812-017-0464-9
  32. Vladimirova ES, Salmin VV, Salmina AB, Oskirko SA, Lazarenko VI. Fluorescence diagnosis of the status of the human lens in vivo. J Appl Spectroscopy. 2012;79(1):126-130. 
  33. Gar’kavenko VV, Salmin VV, Lazarenko VI, Shapovalov KA. Choosing the cataract surgery approach on the basis of spectrofluorometric examination of the of lens. Russian Ophthalmological Journal = Rossiiskii oftal’mologicheskii zhurnal. 2017;10(4):16-19. (In Russ.). https://doi.org/10.21516/2072-0076-2017-10-4-16-19
  34. Neroev VV, Garkavenko VV, Shapiro LA, Salmin VV. Evaluation of the state of retinal electrogenesis during ultraviolet spectrofluorimetric examination of the eye. Russian Ophthalmological Journal = Rossiiskii oftal’mologicheskii zhurnal. 2020;13(2):41-44. (In Russ.). https://doi.org/10.21516/2072-0076-2020-13-2-41-44
  35. Atherton SJ, Lambert C, Schultz J, Williams N, Zigman S. Fluorescence Studies of Lens Epithelial Cells and Their Constituents. Photochem Photobiol. 1999;70(5):823-828.  https://doi.org/10.1111/j.1751-1097.1999.tb08289.x
Contact the author
Email
Message
The publishing house "Media Sphere" does not provide treatment services, does not give recommendations on contacting medical institutions and specific specialists, on the prescription of medicines and dietary supplements.

Email Confirmation

An email was sent to test@gmail.com with a confirmation link. Follow the link from the letter to complete the registration on the site.

Email Confirmation

Contact us
If you have any questions, complaints or suggestions, please use this feedback form.
Email
Message
The publishing house "Media Sphere" does not provide treatment services, does not give recommendations on contacting medical institutions and specific specialists, on the prescription of medicines and dietary supplements.
Submit Application

You can become an author of one of our magazines, send a request and we will consider it in during the day.

Name
Phone
E-mail
Message
Login
Registration
E-mail
Password
Forgot Password?

Log in to the site using your account in one of the services

Password recovery
E-mail
Login
Register

We use cооkies to improve the performance of the site. By staying on our site, you agree to the terms of use of cооkies. To view our Privacy and Cookie Policy, please. click here.