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Bubnova I.A.

Research Institute of Eye Diseases

Surnina Z.V.

Krasnov Research Institute of Eye Diseases

Averich V.V.

Krasnov Research Institute of Eye Diseases

Sarkisova K.G.

Research Institute of Eye Diseases

The effects of corneal collagen cross-linking on the corneal structure in keratoconus

Authors:

Bubnova I.A., Surnina Z.V., Averich V.V., Sarkisova K.G.

More about the authors

Journal: Russian Annals of Ophthalmology. 2020;136(5): 268‑276

DOI: 10.17116/oftalma2020136052268

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Table of contents

THE EFFECTS OF CORNEAL COLLAGEN CROSS-LINKING ON THE CORNEAL STRUCTURE IN KERATOCONUS
THE EFFECTS OF CORNEAL COLLAGEN CROSS-LINKING ON THE CORNEAL STRUCTURE IN KERATOCONUS

To cite this article:

Bubnova IA, Surnina ZV, Averich VV, Sarkisova KG. The effects of corneal collagen cross-linking on the corneal structure in keratoconus. Russian Annals of Ophthalmology. 2020;136(5):268‑276. (In Russ.)
https://doi.org/10.17116/oftalma2020136052268

Подписка 2026 Вестник офтальмологии (Russian Annals of Ophthalmology)
МСФ на ЯМ
Использование инфографики авторами научных работ
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Abstract:

The article reviews the key information regarding morphological changes in keratoconic corneas and dramatic alterations of the corneal tissue induced by corneal cross-linking according to data obtained with in vivo corneal confocal microscopy, presents basic information on keratoconus visualization techniques widely used for diagnosis, monitoring of ectasia, as well as efficacy assessment of its treatment, and lists basic principles of corneal cross-linking procedure and confocal microscopy with consideration of morphology specifics of keratoconic corneas. The article also discusses prospective benefits of further research and longitudinal studies aimed to define the origin of keratoconus and to develop advanced corneal cross-linking protocols.

Keywords:

keratoconus
corneal collagen cross-linking
in vivo corneal confocal microscopy

Authors:

Bubnova I.A.

Research Institute of Eye Diseases

Surnina Z.V.

Krasnov Research Institute of Eye Diseases

Averich V.V.

Krasnov Research Institute of Eye Diseases

Sarkisova K.G.

Research Institute of Eye Diseases

Received:

30.05.2020

Accepted:

13.06.2020

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References:

  1. Avetisov SE. Keratoconus: modern approaches to pathogenetic studies, diagnosis, optical correction and treatment. Vestnik oftal’mologii. 2014;130(6): 37-43. (In Russ.).
  2. Avetisov SE, Novikov IA, Pateiuk LS. Keratoconus: etiological factors and accompanying manifestations. Vestnik oftal’mologii. 2014;130(4):110-116. (In Russ.).
  3. Egorova GB, Rogova AYa. Keratoconus. Diagnostic and monitoring methods. Vestnik oftal’mologii. 2013;129(1):61-66. (In Russ.).
  4. Kasparova EA. Sovremennye predstavleniya ob etiologii i patogeneze keratokonusa. Vestnik oftal’mologii. 2002;118(3):50-53. (In Russ.).
  5. Alio JL. Keratoconus: Recent advances in diagnosis and treatment. Springer; 2016.
  6. Barbara A, Rabinowitz YS. Textbook on keratoconus: New insights. JP Medical Ltd; 2011.
  7. Sinjab MM. Quick guide to the management of keratoconus. Springer; 2011.
  8. Wang M, Swartz TS. Keratoconus and keratoectasia: prevention, diagnosis, and treatment. SLACK Inc; 2010.
  9. Zadnik K, Barr JT, Edrington TB, Everett DF, Jameson M, McMahon TT, Shin JA, Sterling JL, Wagner H, Gordon MO. Baseline findings in the collaborative longitudinal evaluation of keratoconus (CLEK) study. Invest Ophthalmol Vis Sci. 1998;39(13):2537-2546.
  10. Neroev VV, Khandzhyan AT, Oganesyan OG, Penkina AV, Letnikova KB. A comparative analysis of clinical, functional and morphological results of femtosecond, trans-epithelial and standard corneal collagen cross-linking. Rossiiskii oftal’mologicheskii zhurnal. 2017;10(2):47-53. (In Russ.).
  11. Letnikova KB, Khandzhyan AT, Oganesyan OG, Penkina AV, Neroev VV. Femtosecond corneal collagen crosslinking in treatment of patients with progressive keratoconus stages I—II. Sovremennye tehnologii v medicine. 2016; 8(1):128-133. (In Russ.). https://doi.org/10.17691/stm2016.8.1.17
  12. Sitnik HV, Slonimsky AYu, Slonimsky YB. Femtolaser refractive autokeratoplastic: first results and prospects. Oftal’mologiya = Ophthalmology in Russia. 2015;12(3):22-29. (In Russ.).
  13. Mamikonyan VR, Kuznetsov AV, Bubnova IA, Borodina NV, Fedorov AA, Voevodina TM, Budzinskaya MV. Experimental study «cross-linking» of corneal collagen. Byulleten’ Sibirskogo otdeleniya Rossiiskoi akademii meditsinskikh nauk. 2009;138(4):34-36. (In Russ.).
  14. Bikbov MM, Khalimov AR, Usubov EL. Ultraviolet Corneal Crosslinking. Vestnik RAMN = Annals of the Russian Academy of Medical Sciences. 2016; 71(3):224-232. (In Russ.). https://doi.org/10.15690/vramn562
  15. Zotov VV, Pashtaev NP, Pozdeeva NA. Corneal collagen cross-linking for keratoconus. Vestnik oftal’mologii. 2015;131(4):88-93. (In Russ.). https://doi.org/10.17116/oftalma2015131488-93
  16. Neroev VV, Khandzhyan AT, Penkina AV, Sklyarova AS. Primenenie krosslinkinga rogovichnogo kollagena v lechenii keratokonusa I—II stadii. Rossiiskii oftal’mologicheskii zhurnal. 2012;5(1):62-64 (In Russ.).
  17. Bikbov MM, Surkova VK. Corneal collagen crosslinking for keratoconus. A review. Oftal’mologiya = Ophthalmology in Russia. 2014;11(3):13-19. (In Russ.).
  18. Wollensak G, Spoerl E, Seiler T. Riboflavin/ultraviolet-a-induced collagen crosslinking for the treatment of keratoconus. Am J Ophthalmol. 2003;135(5): 620-627.  https://doi.org/10.1016/S0002-9394(02)02220-1
  19. Bubnova IA, Kuznetsov AV, Zelyanina EV. Long-term efficacy of corneal collagen cross-linking in patients with progressive keratoconus. Vestnik oftal’mologii. 2015;131(5):38-42. (In Russ.). https://doi.org/10.17116/oftalma2015131538-42
  20. Mazzotta C, Caporossi T, Denaro R, Bovone C, Sparano C, Paradiso A, Baiocchi S, Caporossi A. Morphological and functional correlations in riboflavin UVA corneal collagen cross-linking for keratoconus. Acta Ophthalmol. 2012;90(3):259-265.  https://doi.org/10.1111/j.1755-3768.2010.01890.x
  21. Masters BR. Noninvasive diagnostic techniques in ophthalmology. NY: Springer-Verlag; 1990.
  22. Bille JF. High resolution imaging in microscopy and ophthalmology. New frontiers in biomedical optics. Springer; 2019.
  23. Sinjab MM. Corneal collagen cross linking. Springer; 2017.
  24. Stein GI. Rukovodstvo po konfokal’noj mikroskopii. SPb: INC RAS; 2007. (In Russ.).
  25. Efron N, Perez-Gomez I, Mutalib HA. Confocal microscopy of the human cornea. Cont Lens Anterior Eye. 2001;24(1):16-24.  https://doi.org/10.1016/s1367-0484(01)80005-9
  26. Avetisov SE, Chernenkova NA, Surnina ZV. Anatomical and functional features of corneal nerve fibers and methods of their evaluation. Vestnik oftal’mologii. 2018;134(6):102-106. (In Russ.). https://doi.org/10.17116/oftalma2018134061102
  27. Surnina ZV. Opportunities for confocal and laser biomicroscopy of corneal nerves in diabetic polyneuropathy. Vestnik oftal’mologii. 2015;131(1):104-108. (In Russ.). https://doi.org/10.17116/oftalma20151311104-108
  28. Avetisov SE, Chernenkova NA, Surnina ZV. Clinical features and diagnosis of diabetic polyneuropathy. Vestnik oftal’mologii. 2017;133(5):98-102. (In Russ.). https://doi.org/10.17116/oftalma2017133598-102
  29. Avetisov SE, Egorova GB, Fedorov AA, Bobrovskikh NV. Confocal microscopy of the cornea. Communication 2. Morphological changes in keratoconus. Vestnik oftal’mologii. 2008;124(3):6-10. (In Russ.).
  30. Egorova GB, Fedorov AA, Bobrovskikh NV, Savochkina OA. Study of corneal morphological changes and light scattering rate in keratoconus. Vestnik oftal’mologii. 2010;126(4):16-20. (In Russ.).
  31. Egorova GB, Rogova AYa. Algoritm diagnostiki subklinicheskoj stadii ektazij rogovicy. Sbornik nauchnyh trudov XI Vserossijskoj shkoly oftal’mologa. 2012: 299-303. (In Russ.).
  32. Egorova GB, Rogova AYa, Mitichkina TS. Diagnostic value of confocal microscopy in primary corneal ectasia. Vestnik oftal’mologii. 2012;128(6):25-29. (In Russ.).
  33. Gumerova SG, Zainullina NB, Garipova EM, Usubov EL. Morfologicheskie aspekty effektivnosti krosslinkinga rogovichnogo kollagena na osnovanii dannykh NRT rogovitsy. Nauchno-prakt. zhurnal «Vostok—Zapad. Tochka zreniya». 2014;1:37-39. (In Russ.).
  34. Moroz ZI, Izmaĭlova SB, Legkikh SL, Merzlov DE. Krosslinking kak metod lecheniya progressiruyushchego keratokonusa. Prakticheskaya meditsina. 2012;1(4):104-106. (In Russ.).
  35. Podtynnykh EV, Basinskaya LA, Komarovskikh EN. Sovremennye predstavleniya ob etiopatogeneze i metodakh diagnostiki keratokonusa (obzor literatury). Vestnik Orenburgskogo gosudarstvennogo universiteta. 2015;187(12):188-196. (In Russ.).
  36. Slepova OS, Moroz ZI, SHilkin GA, Koledincev MN, Legkih LS, Semenova AL. Diagnosticheskie vozmozhnosti immunologicheskogo i biohimicheskogo analiza sleznoj zhidkosti v komplekse s klinikofunkcional’nymi metodami issledovaniya pri keratokonuse. Oftal’mohirurgiya. 2008;4:4-8. (In Russ.).
  37. Patel DV, McGhee CN. Mapping the corneal sub-basal nerve plexus in keratoconus by in vivo laser scanning confocal microscopy. Invest Ophthalmol Vis Sci. 2006;47(4):1348-1351. https://doi.org/10.1167/iovs.05-1217
  38. Mocan MC, Yilmaz PT, Irkec M, Orhan M. In vivo confocal microscopy for the evaluation of corneal microstructure in keratoconus. Curr Eye Res. 2008;33(11-12):933-939.  https://doi.org/10.1080/02713680802439219
  39. Niederer RL, Perumal D, Sherwin T, McGhee CN. Laser scanning in vivo confocal microscopy reveals reduced innervation and reduction in cell density in all layers of the keratoconic cornea. Invest Ophthalmol Vis Sci. 2008; 49(7):2964-2970. https://doi.org/10.1167/iovs.07-0968
  40. Lagali N, Peebo BB, Germundsson J, Eden U, Danyali R, Rinaldo M, Fagerholm P. Confocal laser microscopy: Principles and applications in medicine, biology, and the food sciences. INTECH; 2013. https://doi.org/10.5772/55216
  41. Patel DV, Ku JYF, Johnson R, McGhee CN. Laser scanning in vivo confocal microscopy and quantitative aesthesiometry reveal decreased corneal innervation and sensation in keratoconus. Eye. 2009;23(3);586-592.  https://doi.org/10.1038/eye.2008.52
  42. Gokul A, Vellara HR, Patel DV. Advanced anterior segment imaging in keratoconus: a review. Clin Exp Ophthalmol. 2018;46(2):122-132.  https://doi.org/10.1111/ceo.13108
  43. Ghosh S, Mutalib HA, Kaur S, Ghoshal R, Retnasabapathy S. Corneal cell morphology in keratoconus: a confocal microscopic observation. Malays J Med Sci. 2017;24(2):44-54.  https://doi.org/10.21315/mjms2017.24.2.6
  44. Efron N, Hollingsworth JG. New perspectives on keratoconus as revealed by corneal confocal microscopy. Clin Exp Optom. 2008;91(1):34-55.  https://doi.org/10.1111/j.1444-0938.2007.00195.x
  45. Ucakhan O, Kanpolat A, Ylmaz N, Ozkan M. In vivo confocal microscopy findings in keratoconus. Eye & Contact Lens: Science & Clinical Practice. 2006;32(4):183-191.  https://doi.org/10.1097/01.icl.0000189038.74139.4a
  46. Egorova GB, Fedorov AA, Novikov IA. Morphological Changes in Keratoconus: Interpretation of Corneal Confocal Microscopy Findings. Sovremennye tehnologii v medicine. 2018;10(3):130-138. (In Russ.). https://doi.org/10.17691/stm2018.10.3.16
  47. Pahuja NK, Shetty R, Nuijts RMMA, Agrawal A, Ghosh A, Jayadev C, Nagaraja H. An in vivo confocal microscopic study of corneal nerve morphology in unilateral keratoconus. BioMed Res Int. 2016. https://doi.org/10.1155/2016/5067853
  48. Hollingsworth JG, Efron N, Tullo AB. In vivo corneal confocal microscopy in keratoconus. Ophthalmic and Physiological Optics. 2005;25(3):254-260.  https://doi.org/10.1111/j.1475-1313.2005.00278.x
  49. Weed KH, MacEwen CJ, Cox A, McGhee CN. Quantitative analysis of corneal microstructure in keratoconus utilizing in vivo confocal microscopy. Eye. 2007;21(5):614-623.  https://doi.org/10.1038/sj.eye.6702286
  50. Bitirgen G, Ozkagnici A, Bozkurt B, Malik RA. In vivo corneal confocal microscopic analysis in patients with keratoconus. Int J Ophthalmol. 2015; 8(3):534-539.  https://doi.org/10.3980/j.issn.2222-3959.2015.03.17
  51. Mandathara PS, Stapletona FJ, Kokkinakisa J, Willcoxaa MDP. A pilot study on corneal Langerhans cells in keratoconus. Cont Lens Anterior Eye. 2018; 41(2):219-223.  https://doi.org/10.1016/j.clae.2017.10.005
  52. Mazzotta C, Hafezi F, Kymionis G, Caragiuli S, Jacob S, Traversi C, Barabino S, Randleman JB. In vivo confocal microscopy after corneal collagen cross-linking. Ocul Surf. 2015;13(4):298-314.  https://doi.org/10.1016/j.jtos.2015.04.007
  53. Parissi M, Randjelovic S, Poletti E, Guimaraes P, Ruggeri A, Fragkiskou S, Wihlmark TB, Utheim TP, Lagali N. Corneal nerve regeneration after collagen cross-linking treatment of keratoconus a 5-year longitudinal study. JAMA ophthalmol. 2016;134(1):70-78.  https://doi.org/10.1001/jamaophthalmol.2015.4518
  54. Bikbova GM, Zabolotnaya VA. Gistomorfologiya rogovicy v otdalennyj period posle krosslinkinga po povodu keratokonusa. Sbornik nauchnyh trudov konferencii s mezhdunarodnym uchastiem po oftal’mohirurgii «Vostok—Zapad». 2011:64-67. (In Russ.).
  55. Mazzotta C, Traversi C, Baiocchi S, Caporossi O, Bovone C, Sparano MC, Balestrazzi A, Caporossi A. Corneal healing after riboflavin ultraviolet-A collagen cross-linking determined by confocal laser scanning microscopy in vivo: early and late modifications. Am J Ophthalmol. 2008;146(4):527-533.  https://doi.org/10.1016/j.ajo.2008.05.042
  56. Mazzotta C, Balestrazzi A, Traversi C, Baiocchi S, Caporossi T, Tommasi C, Caporossi A. Treatment of progressive keratoconus by riboflavin-UVA-induced cross-linking of corneal collagen: ultrastructural analysis by Heidelberg Retinal Tomograph II in vivo confocal microscopy in humans. Cornea. 2007;26(4):390-397.  https://doi.org/10.1097/ico.0b013e318030df5a
  57. Jordan C, Patel DV, Abeysekera N, McGhee CN. In vivo confocal microscopy analyses of corneal microstructural changes in a prospective study of collagen cross-linking in keratoconus. Ophthalmology. 2014;121(2):469-474.  https://doi.org/10.1016/j.ophtha.2013.09.014
  58. Kymionis GD, Diakonis VF, Kalyvianaki M, Portaliou D, Siganos C, Kozobolis VP, Pallikaris AI. One-year follow-up of corneal confocal microscopy after corneal cross-linking in patients with post-laser in situ keratosmileusis ectasia and keratoconus. Am J Ophthalmol. 2009;147:774-778.  https://doi.org/10.1016/j.ajo.2008.11.017
  59. Ozgurhan EB, Sezgin Akcay BI, Yildirim Y, Karatas G, Kurt T, Demirok A. Evaluation of corneal stromal demarcation line after two different protocols of accelerated corneal collagen cross-linking procedures using anterior segment optical coherence tomography and confocal microscopy. J Ophthalmol. 2014. https://doi.org/10.1155/2014/981893
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