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Avetisov S.É.

FGBU "Nauchno-issledovatel'skiĭ institut glaznykh bolezneĭ" RAMN, Moskva

Trufanov S.V.

Uchrezhdenie Rossiĭskoĭ akademii meditsinskikh nauk "NII glaznykh bolezneĭ RAMN", Moskva

Novikov I.A.

Institute of Cardiology named after A.L. Myasnikov of National Medical Research Center for Cardiology, Moscow, Russia

Subbot A.M.

FGBU "NII glaznykh bolezneĭ" RAMN, Moskva

Fedorov A.A.

Moskovskiĭ oblastnoĭ NII akusherstva i ginekologii

SEM visualization of corneal epithelium through lanthanoid staining based on Ca/Nd isomorphous substitution in Ca-dependent molecular systems

Authors:

Avetisov S.É., Trufanov S.V., Novikov I.A., Subbot A.M., Fedorov A.A.

More about the authors

Journal: Russian Annals of Ophthalmology. 2016;132(6): 11‑19

DOI: 10.17116/oftalma2016132611-19

Read: 1772 times

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Table of contents

SEM VISUALIZATION OF CORNEAL EPITHELIUM THROUGH LANTHANOID STAINING BASED ON CA/ND ISOMORPHOUS SUBSTITUTION IN CA-DEPENDENT MOLECULAR SYSTEMS
SEM VISUALIZATION OF CORNEAL EPITHELIUM THROUGH LANTHANOID STAINING BASED ON CA/ND ISOMORPHOUS SUBSTITUTION IN CA-DEPENDENT MOLECULAR SYSTEMS

To cite this article:

Avetisov SÉ, Trufanov SV, Novikov IA, Subbot AM, Fedorov AA. SEM visualization of corneal epithelium through lanthanoid staining based on Ca/Nd isomorphous substitution in Ca-dependent molecular systems. Russian Annals of Ophthalmology. 2016;132(6):11‑19. (In Russ.)
https://doi.org/10.17116/oftalma2016132611-19

Подписка 2026 Вестник офтальмологии (Russian Annals of Ophthalmology)
 
МСФ на ЯМ
Использование инфографики авторами научных работ
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Abstract:

Cumulative biomicroscopic evidence is usually sufficient for the diagnosis of recurrent corneal erosion or bullous keratopathy, however, exploration of the disease pathogenesis requires subcellular-level visualization of corneal structure. In the current study, lanthanoid staining and scanning electron microscopy were employed to visualize quite a number of structures responsible for epithelium organization. In particular, the study proves possible the use of Ca/Nd isomorphous substitution at Ca2+ sites of cytoadherence proteins for visualization of corresponding cellular structures. Aim — to assess the value of information provided by scanning electron microscopy of corneal epithelium that involves lanthanoid staining based on the Ca/Nd isomorphous substitution in Ca-dependent molecular systems. Material and methods. Anterior corneal epithelial scrapes were obtained from patients with recurrent corneal erosion or bullous keratopathy and cadaver eyes with no signs of any ophthalmic disease. Samples were then studied under a scanning electron microscope (Zeiss EVO LS10, BSE, EP — 79 Pa, 20—28 kV, Ln-staining with the BioREE assay kit). Results. In all cases, lanthanoid staining of biopsy material provided high-contrast SEM images with well-recognizable structural and ultrastructural elements associated with Ca2+ sites of cytoadherence proteins. Conclusion. Lanthanoid staining of biopsy material and subsequent SEM enabled detailed visualization of structural features of the corneal epithelium in various pathologies. Due to the Ca/Nd isomorphism we were able to evaluate structural position of the majority of protein molecules engaged in Ca-dependant processes and, consequently, in cytoadherence. Basing on the neodymium distribution within the basal membrane, we have described local effects of different substances on the lamina densa in the projection of basal layer cell borders that occur after unidirectional ultrafiltration. The results confirm the failure of the junctional adhesion complex in recurrent corneal erosion.

Keywords:

epithelium
cornea
bullous keratopathy
recurrent corneal erosion
scanning electron microscopy
SEM
scanning electron microscope
lanthanoid staining
cell adhesion molecules

Authors:

Avetisov S.É.

FGBU "Nauchno-issledovatel'skiĭ institut glaznykh bolezneĭ" RAMN, Moskva

Trufanov S.V.

Uchrezhdenie Rossiĭskoĭ akademii meditsinskikh nauk "NII glaznykh bolezneĭ RAMN", Moskva

Novikov I.A.

Institute of Cardiology named after A.L. Myasnikov of National Medical Research Center for Cardiology, Moscow, Russia

Subbot A.M.

FGBU "NII glaznykh bolezneĭ" RAMN, Moskva

Fedorov A.A.

Moskovskiĭ oblastnoĭ NII akusherstva i ginekologii

Close metadata

References:

  1. Mannis MJ, Holland EJ. Ocular Surface Disease: Medical and Surgical Management. Springer Science & Business Media; 2006:59-62.
  2. Trufanov S, Malozhen S, Polunina E, Pivin E, Tekeeva L. Recurrent corneal erosion syndrome (a review). Oftal'mologija. 2015;12(2):4-12. (In Russ.)
  3. Heyworth P, Morlet N, Rayner S, Hykin P, Dart J. Natural history of recurrent erosion syndrome — a 4 year review of 117 patients. Br J Ophthalmol. 1998;82:26-28.
  4. Reidy JJ, Paulus MP, Gona S. Recurrent erosions of the cornea: epidemiology and treatment. Cornea. 2000;19:767-771. doi:10.1016/S0002-9394(01)00884-4
  5. Mencucci R, Paladini I, Brahimi B, Menchini U, Dua HS, Romagnoli P. Alcohol delamination in the treatment of recurrent corneal erosion: an electron microscopic study. Br J Ophthalmol. 2010;94(7):933-939. doi:10.1136/bjo.2009.174409
  6. Pal-Ghosh S, Blanco T, Tadvalkar G, Pajoohesh-Ganji A, Parthasarathy A, Zieske JD et al. MMP9 cleavage of the β4 integrin ectodomain leads to recurrent epithelial erosions in mice. J Cell Sci. 2011;124(Pt 15):2666-2675. doi:10.1242/jcs.085480
  7. Oliver VF, van Bysterveldt KA, Cadzow M, Steger B, Romano V, Markie D et al. A COL17A1 Splice-Altering Mutation Is Prevalent in Inherited Recurrent Corneal Erosions. Ophthalmology. 2016;123(4):709-722. doi:10.1016/j.ophtha.2015.12.008
  8. Garrana RM, Zieske JD, Assouline M, Gipson IK. Matrix metalloproteinases in epithelia from human recurrent corneal erosion. Invest Ophthalmol Vis Sci. 1999;40(6):1266-1270.
  9. Ljubimov AV, Burgeson RE, Butkowski RJ, Couchman JR, Wu RR, Ninomiya Y et al. Extracellular matrix alterations in human corneas with bullous keratopathy. Invest Ophthalmol Vis Sci. 1996;37(6):997-1007.
  10. Savige J, Sheth S, Leys A, Nicholson A, Mack HG, Colville D. Ocular features in Alport syndrome: pathogenesis and clinical significance. Clin J Am Soc Nephrol. 2015;10(4):703-799. doi:10.2215/CJN.10581014
  11. Akhtar S, Bron A, Hawksworth N, Bonshek R, Meek K. Ultrastructural morphology and expression of proteoglycans, βig-h3, tenascin-C, fibrillin-1, and fibronectin in bullous keratopathy. Br J Ophthalmol. 2001;85(6):720-731. doi:10.1136/bjo.85.6.720
  12. Torricelli AAM, Singh V, Santhiago MR, Wilson SE. The Corneal Epithelial Basement Membrane: Structure, Function, and Disease. Invest Opthalmol Vis Sci 2013;54(9):6390. doi:10.1167/iovs.13-12547
  13. Novikov I, Subbot A, Fedorov A, Griboedova I., Antonov E, Vakhrushev I. Supravital lanthanoid staining for scanning electron microscopy of biological objects. Geny i kletki. 2015;10(2):90-96. (In Russ.)
  14. Novikov IA, Subbot AM, Kiryushchenkova NP, Nesterova TV, Gabashvili AN, Sitnikov AV, и др. Fast and easy method of lanthanoid staining for visualization of cellular ultrastructure and spatial arrangement. В: AIP Conference Proceedings. AIP Publishing; 2016 с. 20009. http://scitation.aip.org/content/aip/proceeding/aipcp/10.1063/1.4954343;jsessionid=kbwfzGpq96qMxc-Ro31dkHwy.x-aip-live-02
  15. Ferreira-Gomes MS, González-Lebrero RM, de la Fuente MC, Strehler EE, Rossi RC, Rossi JPFC. Calcium occlusion in plasma membrane Ca2+-ATPase. J Biol Chem. 2011;286(37):32018-32025. doi:10.1074/jbc.M111.266650
  16. Cailliez F, Lavery R. Cadherin Mechanics and Complexation: The Importance of Calcium Binding. Biophys J. 2005;89(6):3895-3903. doi:10.1529/biophysj.105.067322
  17. Burton PR, Fernandez HL. Delineation by Lanthanum Staining of Filamentous Elements Associated with the Surfaces of Axonal Microtubules. Journal of Cell Science. 1973;12(2):567-583.
  18. Evans CH. Biochemistry of the Lanthanides. Springer Science & Business Media; 2013.
  19. Rubashkin A, Iserovich P, Hernández JA, Fischbarg J. Epithelial fluid transport: protruding macromolecules and space charges can bring about electro-osmotic coupling at the tight junctions. J Membr Biol. 2005;208(3):251-263. doi:10.1007/s00232-005-0831-y
  20. Aitken DA, Beirouty ZA, Lee WR. Ultrastructural study of the corneal epithelium in the recurrent erosion syndrome. Br J Ophthalmol. 1995;79(3):282-289.
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