Establishing the dynamics of activity and stability of total PSA samples in semen samples on gauze, diluted with distilled water

Gusarov A.A.; Sidorov V.L.; Yagmurov O.D.; Momynaliev K.T.

doi:https://doi.org/10.17116/sudmed20256805139

Gusarov A.A.

Russian Centre of Forensic Medical Expertise

ORCID: 0000-0003-4270-1609

Sidorov V.L.

Bureau of Forensic Medicine;
All-Russian Research and Testing Institute of Medical Equipment of Roszdravnadzor

ORCID: 0000-0002-2214-895X

Yagmurov O.D.

Bureau of Forensic Medicine

ORCID: 0000-0002-8141-4488

Momynaliev K.T.

All-Russian Scientific Research and Testing Institute for Medical Devices

ORCID: 0000-0003-4656-1025

Establishing the dynamics of activity and stability of total PSA samples in semen samples on gauze, diluted with distilled water

Authors:

Gusarov A.A., Sidorov V.L., Yagmurov O.D., Momynaliev K.T.

More about the authors

Journal: Forensic Medical Expertise. 2025;68(5): 39‑42

DOI: 10.17116/sudmed20256805139

Read: 242 times

Contact the author

Table of contents

To cite this article:

Gusarov AA, Sidorov VL, Yagmurov OD, Momynaliev KT. Establishing the dynamics of activity and stability of total PSA samples in semen samples on gauze, diluted with distilled water. Forensic Medical Expertise. 2025;68(5):39‑42. (In Russ.)
https://doi.org/10.17116/sudmed20256805139

Подписка 2026 Судебно-медицинская экспертиза (Forensic Medical Expertise)

Использование инфографики авторами научных работ

Recommended articles:

Method of linear calibration function control according to GOST R ISO 11095—2007 for measuring the mass concentration of ethanol. Forensic Medical Expertise. 2025;(1):43-49

Detection of mpox virus by enzyme immunoassay in a no-instrumental format and after its inactivation with formaldehyde. Molecular Genetics, Microbiology and Virology. 2024;(4):49-53

Assessment of stability of maxillary advancement using the distraction method in patients with cleft lip and palate. Russian Rhinology. 2025;(2):131-136

Intercellular adhesion proteins: P-, E-, H-cadherins as oncological and cardiovascular diseases’ biomarkers. Russian Journal of Preventive Medicine. 2025;(7):100-105

Philosophy in pathological anatomy. Russian Journal of Archive of Pathology. 2025;(4):62-67

Comparison of pH in teeth bleaching systems. Stomatology. 2025;(5):12-17

Abstract:

The article presents the results of an experimental study of the stability of total PSA in samples from sperm dried on gauze, diluted with distilled water. The goal of the work was to establish the dynamics of activity and stability of total PSA samples in the prepared extracts. The research material included samples of seminal fluid dried on gauze. The study was carried out using a quantitative enzyme-linked immunosorbent assay. Measurements in the samples were carried out 5 times during the working day. The results obtained allowed us to prove a significant decrease in total PSA activity in extracts from sperm stains on gauze for 6 hours at room temperature (+18—20 °C). The results of the study, confirmed by statistical analysis data, can be taken into account in the case of using the quantitative enzyme immunoassay method to determine the presence of sperm on material evidence during forensic biological examinations.

Keywords:

total PSA

enzyme immunoassay

stability

activity

concentration

distilled water

Authors:

Gusarov A.A.

Russian Centre of Forensic Medical Expertise

ORCID: 0000-0003-4270-1609

Sidorov V.L.

Bureau of Forensic Medicine;
All-Russian Research and Testing Institute of Medical Equipment of Roszdravnadzor

ORCID: 0000-0002-2214-895X

Yagmurov O.D.

Bureau of Forensic Medicine

ORCID: 0000-0002-8141-4488

Momynaliev K.T.

All-Russian Scientific Research and Testing Institute for Medical Devices

ORCID: 0000-0003-4656-1025

Received:

17.09.2024

Accepted:

29.09.2024

Close metadata

References:

Sidorov VL, Gusarov AA, Yagmurov OD. Modern expert algorithms for studying traces of blood, sperm and saliva on physical evidence. Vestnik Vserossijskogo obshhestva specialistov po mediko-social’noj jekspertize, reabilitacii i reabilitacionnoj industrii. 2017;4:70-81. (In Russ.).
Sidorov VL, Loban IE, Gusarov AA, Portnova NA, Khorovskaya LA. Comparative characteristics of methods for studying physical evidence used to establish the presence of blood and secretions in the Russian Federation and in foreign countries. Vestnik sudebnoy meditsiny. 2020;9(1):10-16. (In Russ.).
Sidorov VL, Loban IE, Gusarov AA, Portnova NA, Khorovskaya LA. Application of quantitative methods for studying traces of blood and secretions on material evidence during forensic biological examinations. Vestnik sudebnoy meditsiny. 2020;9(2):28-34. (In Russ.).
Gusarov AA, Sidorov VL, Yagmurov OD, Nagornov MN. Methodology for performing intralaboratory quality control in establishing species of the blood by IgGtotal with the use of quantitative enzyme-linked immunosorbent assay. Forensic Medical Expertise. 2025;68(1):38-42. (In Russ.). https://doi.org/10.17116/sudmed20256801138
Gusarov AA, Sidorov VL, Surikova NE, Yagmurov OD. Organ and species identification of liver microparticles using forensic cytology and quantitative enzyme-linked immunosorbent assay methods. Forensic Medical Expertise. 2025;68(4):29-33. (In Russ.). https://doi.org/10.17116/sudmed20256804129
Gusarov AA, Sidorov VL, Yagmurov OD, Surikova NE. Establishing the species belonging of fragments and particles of striated muscle tissue by total IgG using quantitative solid-phase enzyme-linked immunosorbent assay. Forensic Medical Expertise.2024;67(6):21-24. (In Russ.). https://doi.org/10.17116/sudmed20246706121
Gusarov AA, Surikova NE. Metodika proizvodstva sudebno-citologicheskikh issledovaniy po delam o polovykh prestuplenijakh. Uchebnoe posobie. Moskva; 2023. (In Russ.).
Sidorov VL, Gusarov AA, Isakova IV, Yagmurov OD. Establishing the species affiliation of biological objects based on human IgG using quantitative solid-phase enzyme-linked immunosorbent assay: improved medical technology. M.: RCFME; 2011. (In Russ.).
Fokina EV, Gusarov AA. Forensic biological examination in road traffic accidents. Meditsinskaja jekspertiza i pravo. 2011;4:20-22. (In Russ.).
Sidorov VL, Gusarov AA, Isakova IV. Determination of the presence of sperm in material evidence using the acid phosphatase colorimetric method: guidelines. M.: RCFME; 2012. (In Russ.).
Gusarov AA, Fokina EV. A rare case of a car injury with a tree stump penetrating into the car interior. Medicinskaja jekspertiza i pravo. 2011;3:52-53. (In Russ.)
Fetisov VA, Gusarov AA, Khabova ZS, Smirenin SA. Modern problems of damage research in publications of the journal Forensic Medical Examination (2000—2014). Sudebno-meditsinskaya ekspertiza. 2015;58(4):56–62. (In Russ.).
Sidorov VL, Yagmurov OD, Gusarov AA. Methods of using quantitative enzyme-linked immunosorbent assay to determine the species of biological objects and to detect sperm on physical evidence. Vestnik sudebnoy meditsiny. 2021;10(2):4-8. (In Russ.).
Sidorov VL, Yagmurov OD, Gusarov AA, Portnova NA. The application of the colorimetric method for detecting sperm and saliva based on material evidence. Vestnik sudebnoy meditsiny. 2021;10(1):22-26. (In Russ.).
Sidorov VL, Loban IE, Gusarov AA, Isakova IV, Khorovskaya LA, Portnova NA. Algorithm for studying traces of blood and secretions on material evidence using quantitative enzyme-linked immunosorbent assay and colorimetry: methodological recommendations. M.: RCFME; 2021. (In Russ.).
Sidorov VL, Gusarov AA, Isakova IV, Cherepanova TV, Spiridonova OV. Detection of the presence of sperm in human prostate-specific antigen evidence using quantitative enzyme-linked immunosorbent assay: an improved medical technology. M.: RCFME; 2011. (In Russ.).