The identification and quantitative measurement of mirtazapine isolated from the biological material by high performance liquid chromatography

Darmograĭ N.N.; Gal'kevich I.I.

doi:https://doi.org/

Darmograĭ N.N.

Kafedra toksikologicheskoĭ i analiticheskoĭ khimii L'vovskogo natsional'nogo meditsinskogo universiteta im. Daniila Galitskogo, Ukraina

Gal'kevich I.I.

Kafedra toksikologicheskoĭ i analiticheskoĭ khimii L'vovskogo natsional'nogo meditsinskogo universiteta im. Daniila Galitskogo, Ukraina

The identification and quantitative measurement of mirtazapine isolated from the biological material by high performance liquid chromatography

Authors:

Darmograĭ N.N., Gal'kevich I.I.

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Journal: Forensic Medical Expertise. 2014;57(1): 37‑40

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To cite this article:

Darmograĭ NN, Gal'kevich II. The identification and quantitative measurement of mirtazapine isolated from the biological material by high performance liquid chromatography. Forensic Medical Expertise. 2014;57(1):37‑40. (In Russ.)

Подписка 2026 Судебно-медицинская экспертиза (Forensic Medical Expertise)

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Abstract:

The present study was designed to estimate the effectiveness of isolation of mirtazapine from the liver, blood, and urine. The conditions were developed for the identification and quantitative measurement of the isolated mirtazapine with the use of high performance liquid chromatography and detection from the UV-spectrum and mass-spectrometry. The retention time of mirtazapine isolated from the liver was 2.88±0.08 min. The straight-line equation within the range of mirtazapine concentrations from 1 to 20 mc/ml was characterized by the dependence: Y=3.25 × 104X - 6.27 × 103 (r=0.9997). The study showed that it is possible to isolate 46.44±1.89% of mirtazapine present in the liver and 50.4±1.05% from blood using extraction by acetonitrile acidified with an 1 M hydrochloric acid solution. Chloroform extraction from urine at pH 7.0-8.0 releases 90.22±1.88% of mirtazapine.

Keywords:

mirtazapine

high performance liquid chromatography

isolation

biological material

Authors:

Darmograĭ N.N.

Kafedra toksikologicheskoĭ i analiticheskoĭ khimii L'vovskogo natsional'nogo meditsinskogo universiteta im. Daniila Galitskogo, Ukraina

Gal'kevich I.I.

Kafedra toksikologicheskoĭ i analiticheskoĭ khimii L'vovskogo natsional'nogo meditsinskogo universiteta im. Daniila Galitskogo, Ukraina

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