Morphological features of reparative regeneration in inflammatory response of the nasal mucosa after injury: an experimental study

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Surgical interventions on the nose’s structures are accompanied by mucosal membrane traumatization with inflammatory reaction development. A pathological regeneration develops in case of final inflammatory stage disorder. Study of wound healing process patterns is promising for development of pathogenetically based treatment for complete morphofunctional epithelium recovery.

OBJECTIVE

To investigate the morphological features of reparative regeneration of the nasal mucosa after injury in an experiment.

MATERIAL AND METHODS

An experimental study was conducted on 240 mature white male rates at the laboratory of the Physiology Department in St. Luke Lugansk State Medical University. All animals were divided into 3 groups (80 animals in each): control group; the 1st exp erimental group (comparison), in which treatment wasn’t carried out after injury simulation; the 2nd experimental group, in which anti-inflammatory therapy was prescribed. Animals in the number of 10 in each group were taken out of the experiment on the 2nd, 5th, 10th, 14th, 21st, 30th, 42nd, 60th day. Morphometric study of the nasal mucosa and immunohistochemical examination were performed to characterize the proportion of proliferating cells by Ki-67 expression.

RESULTS

Alteration processes were predominant, more pronounced in the comparison group in microscope slides of both experimental groups on the 2—5th day. There have been signs of regeneration in the form of undifferentiated epithelial foci occurrence from the 10th—14th day. A tendency to increase of goblet cells has been noted in the comparison group already from the 14th day. On the 30th day, foci of granulating tissue appeared at the site of ulceration in the slides of both experimental groups, foci of fibrosis appeared in stroma. Signs of pathological regeneration were revealed on the 60th day in both groups, more pronounced in the comparison group.

CONCLUSION

Morphological examination of the nasal mucosa s after injury even with prescribed anti-inflammatory therapy showed signs of impaired reparative regeneration.

Keywords:

mucous membrane

injury

nasal cavity

reparative regeneration

pathological regeneration

Authors:

Berest I.Ye.

St. Luke Lugansk State Medical University

ORCID: 0000-0003-1694-341X

Received:

13.12.2023

Accepted:

20.01.2024

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