Analysis of the methylation of the promoter regions of two genes-oncosuppressors KLF11 and KRT19 in the tissues of leiomyomas and myometrium showed that both of these genes are methylated in myomatous nodes, which causes a decrease in their expression and stimulates tumor development. At the same time, these genes are not methylated in the tissues of the myometrium. The promoter of the KRT19 gene, unlike the KLF11 promoter, is also methylated in adenomyosis samples, which shows at the gene level the presence of both similarities and differences in the molecular mechanisms regulating the development of adenomyosis and the growth of myomatous nodes.
THE PURPOSE OF THE STUDY
Analysis of methylation of promoter regions of KLF11 and KRT19 genes in leiomyoma and myometrial tissues. Both two genes are oncosuppressors, so its methylation can play an important role in the occurrence and growth of fibroids.
MATERIALS AND METHODS
Tissue samples of myoma nodes and myometrium were collected during laparoscopic myomectomy, as well as blood aliquots from patients with uterine fibroids. DNA isolated from tissues after bisulfite conversion and PCR of two genome sites was sequenced to determine the methylation status of these sites in each sample. DNA samples from blood, adenomyotic foci, as well as placenta and amniotic fluid were also examined as control samples.
RESULTS
Methylation of the promoter regions of the KLF11 and KRT19 genes was detected for the majority of fibroid samples. There is no such methylation in myometrial samples. The results obtained indicate the functional role of silencing genes regulated by methylation of the promoter regions of the KLF11 and KRT19 genes in the pathogenesis of uterine leiomyoma.
CONCLUSION
Methylation of the promoter regions of the KLF11 and KRT19 genes was confirmed in a sample of patients from the Russian population, which will allow further use of such methylation as a marker of the transition of myometrium to myoma tissue, as well as further studies of the functional role of gene methylation in the development of fibroids.
