Angiogenic properties of endometrial aquaporins in patients with peritoneal endometriosis

Burlev V.A.; Il'iasova N.A.

doi:https://doi.org/10.17116/repro201723319-31

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Abstract:

Objective — to study the AQP1, AQP2, VEGF-A, sVEGF-R2 content in the blood and peritoneal fluid, to estimate the intensity of AQP1, AQP2, VEGF-A, VEGF-R2 expression in vessels of eutopic and ectopic endometrium and the density of microvessel in norm and in patients with endometriosis. Material and methods. Immunohistochemistry was used for expression studies AQP1, AQP2, VEGF-A, VEGF-R2 and the density of microvessel in eutopic and ectopic endometrium in women with endometriosis. ELISA method was used to determine the concentration in serum AQP1, AQP2, VEGF-A, sVEGF-R2 and peritoneal fluid. Results. The results showed a statistically significant decrease in the AQP1 content and an increase in AQP2 in the blood and peritoneal fluid in patients with peritoneal endometriosis versus control. The AQP1 content in the peritoneal fluid was statistically significantly higher in patients with endometriosis with respect to blood. At the same time, there were no differences in the content of AQP2 in the control between blood and peritoneal fluid. In patients with endometriosis, an increase in the AQP2 content in the peritoneal fluid with respect to blood was observed. The expression of AQP1 in the vessels of the eutopic and ectopic endometrium was different and statistically significantly reduced with respect to control. It is noted that this expression was the lowest and statistically significant in the ectopic endometrium. The expression of AQP2 in eutopic and ectopic endometrium was statistically significantly increased with respect to control. A statistically significant increase in the expression of AQP2 in the ectopic endometrium was observed between the eutopic and ectopic endometrium. Conclusion. The content of AQP1 and AQP2 in the blood and peritoneal fluid, and the expression of AQP1 and AQP2 in eutopic and ectopic endometrium can be used to characteristics systemic and local damage of angiogenesis in endometriosis.

Keywords:

endometriosis

eutopic endometrium

ectopic endometrium

aquaporins

Authors:

Burlev V.A.

Nauchnyĭ tsentr akusherstva, ginekologii i perinatologii im. akad. V.I. Kulakova, Moskva

Il'iasova N.A.

Nauchnyĭ tsentr akusherstva, ginekologii i perinatologii im. akad. V.I. Kulakova, Moskva

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