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Smirnova N.I.

FSSI Russian Anti-Plague Institute «Microbe» of the Rospotrebnadzor

Rybal’chenko D.A.

FSSI Russian Anti-Plague Institute «Microbe» of the Rospotrebnadzor

Lozovsky Yu.V.

FSSI Russian Anti-Plague Institute «Microbe» of the Rospotrebnadzor

Kutyrev V.V.

Russian Research Anti-Plague Institute “Microbe”

Genome instability of the cholera agent: role in the evolution of pathogenicity, drug resistance and adaptation to a changing environment

Authors:

Smirnova N.I., Rybal’chenko D.A., Lozovsky Yu.V., Kutyrev V.V.

More about the authors

Journal: Molecular Genetics, Microbiology and Virology. 2025;43(2): 3‑13

DOI: 10.17116/molgen2025430213

Read: 1769 times

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Table of contents

GENOME INSTABILITY OF THE CHOLERA AGENT: ROLE IN THE EVOLUTION OF PATHOGENICITY, DRUG RESISTANCE AND ADAPTATION TO A CHANGING ENVIRONMENT
GENOME INSTABILITY OF THE CHOLERA AGENT: ROLE IN THE EVOLUTION OF PATHOGENICITY, DRUG RESISTANCE AND ADAPTATION TO A CHANGING ENVIRONMENT

To cite this article:

Smirnova NI, Rybal’chenko DA, Lozovsky YuV, Kutyrev VV. Genome instability of the cholera agent: role in the evolution of pathogenicity, drug resistance and adaptation to a changing environment. Molecular Genetics, Microbiology and Virology. 2025;43(2):3‑13. (In Russ.)
https://doi.org/10.17116/molgen2025430213

Подписка 2026 Молекулярная генетика, микробиология и вирусология (Molecular Genetics, Microbiology and Virology)
МСФ на ЯМ
Использование инфографики авторами научных работ
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Abstract:

Cholera, which has claimed millions of human lives, currently continues to pose a particular danger, since the evolution of the genome of the causative agent of the current 7th pandemic has resulted in the emergence of genetically modified strains with increased pathogenic and epidemic potential. A review of current data on the instability of the genome of Vibrio cholerae biovar El Tor associated with the presence of various mobile genetic elements is presented. The molecular-genetic mechanisms underlying the evolution of the pathogen in the modern period are considered. The role of horizontal gene transfer and subsequent mutations in the emergence of new variants of the pathogen with altered genes of pathogenicity, antibiotic resistance and pandemicity is shown. The biological consequences of these changes in the genome associated with the enhancement of the virulence properties of modern variants of the pathogen, the emergence of multiple drug resistance and their widespread distribution are discussed. A number of priority areas in further research of the pathogen at the genomic, transcriptomic and proteomic levels are also presented.

Keywords:

Vibrio cholerae
genetic variants
evolution
genome
mobile genetic elements
virulence
resistance
epidemic potential
genomic diversity

Authors:

Smirnova N.I.

FSSI Russian Anti-Plague Institute «Microbe» of the Rospotrebnadzor

Rybal’chenko D.A.

FSSI Russian Anti-Plague Institute «Microbe» of the Rospotrebnadzor

Lozovsky Yu.V.

FSSI Russian Anti-Plague Institute «Microbe» of the Rospotrebnadzor

Kutyrev V.V.

Russian Research Anti-Plague Institute “Microbe”

Received:

04.03.2025

Accepted:

24.04.2025

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References:

  1. Mekalanos JJ, Rubin, EJ, Waldor MK. Cholera: molecular basis for emergence and pathogenesis. FEMS immunology and medical microbiology. 1997;18(4):241-248.  https://doi.org/10.1111/j.1574-695X.1997.tb01052.x
  2. Montero DA, Vidal RM, Velasco J, George S, Lucero Y, Gómez LA, et al. Vibrio cholerae, classification, pathogenesis, immune response, and trends in vaccine development. Frontiers in medicine. 2023;10:1155751. https://doi.org/10.3389/fmed.2023.1155751
  3. Hu D, Liu B, Feng L, Ding P, Guo X, Wang M, et al. Origins of the current seventh cholera pandemic. Proc Natl Acad Sci USA. 2016;113(48):E7730-E7739. https://doi.org/10.1073/pnas.1608732113
  4. Mutreja A, Kim D, Thomson N, Connor TR, Lee JH, Kariuki S, et al. Evidence for several waves of global transmission in the seventh cholera pandemic. Nature. 2011;477(7365):462-5  https://doi.org/10.1038/nature10392
  5. Heidelberg JF, Eisen JA, Nelson WC, Clayton RA, Gwinn ML, Dodson RJ, et al. DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae. Nature. 2000;406(6795):477-483.  https://doi.org/10.1038/35020000
  6. Dziejman M, Balon E, Boyd D, Fraser CM, Heidelberg JF, Mekalanos JJ, et al. Comparative genomic analysis of Vibrio cholerae: genes that correlate with cholera endemic and pandemic disease. Proc Natl Acad Sci USA. 2002;99(3):1556-1561. https://doi.org/10.1073/pnas.042667999
  7. Smirnova NI, Zadnova SP, Agafonov DA, Shashkova AV, Cheldyshova NB, Cherkasov AV. Comparative molecular-genetic analysis of mobile elements in natural strains of cholera agent. Russian Journal of Genetics. 2013;49(9):1036-47. (In Russ.). https://doi.org/10.7868/S0016675813090087
  8. Pant A, Bag S, Saha B, Verma J, Kumar P. Banerjee S, et al. Molecular insights into the genome dynamics and interactions between core and acquired genomes of Vibrio cholerae. Proc Natl Acad Sci USA. 2020;117(38): 23762-23773. https://doi.org/10.1073/pnas.2006283117
  9. De R. Mobile genetic elements of Vibrio cholerae and the evolution of its antimicrobial resistance. Front. Trop. Dis. 2021;(2):691604. https://doi.org/10.3389/fitd.2021.691604
  10. Ilyina TS. Filamentous bacteriophages and their role in the virulence and evolution of pathogenic bacteria. Molecular Genetics, Microbiology and Virology. 2015;30(1):1-10.  https://doi.org/10.3103/S0891416815010036
  11. Kim EJ., Lee D, Moon SH, Lee CH, Kim SJ, Lee JH, et al. Molecular insights into the evolutionary pathway of Vibrio cholerae O1 atypical El Tor variants. PLOS Pathogens. 2014;10(9):e1004384. https://doi.org/10.1371/journal.ppat.1004384
  12. Verma J, Bag S, Saha B, Kumar P., Ghosh TS, Dayal M, et al. Genomic plasticity associated with antimicrobial resistance in Vibrio cholerae. Proc Natl Acad Sci USA. 2019;116(13):6226-6231. https://doi.org/10.1073/pnas.1900141116
  13. Safa A, Nair GB, Kong RY. Evolution of new variants of Vibrio cholerae O1. Trends in microbiology. 2010;18(1):46-54.  https://doi.org/10.1016/j.tim.2009.10.003
  14. Chin CS, Sorenson J, Harris JB, Robins WP, Charles RC, Jean-Charles RR, et al. The origin of the Haitian cholera outbreak strain. The New England journal of medicine. 2011;364(1):33-4.  https://doi.org/10.1056/NEJMoa1012928
  15. Bhandari M, Jennison AV, Rathnayake IU, Huygens F. Evolution, distribution and genetics of atypical Vibrio cholerae — A review. Infection, genetics and evolution. 2021;(89):104726. https://doi.org/10.1016/j.meegid.2021.104726
  16. Smirnova NI, Badanin DV, Rybal’chenko DA, Krasnov YaM, Kritsky AA, Lozovsky YuV, et al. Variability of the genome of El Tor cholera vibrios isolated before the onset and in different periods of the current pandemic. Molecular Genetics, Microbiology and Virology. 2021;39(2):25-37. (In Russ.). https://doi.org/10.17116/molgen20213902125
  17. Akimkin VG, Semenenko TA., Khafizov KF, Ugleva SV, Dubodelov DV, Kolosovskaya EN. Genomic surveillance strategy. Problems and perspectives. Journal of microbiology, epidemiology and immunobiology. 2024; 101(2):163-172.  https://doi.org/10.36233/0372-9311-507
  18. Waldor MK, Mekalanos JJ. Lysogenic conversion by a filamentous phage encoding cholera toxin. Science. 1996;272(5270):1910-1914. https://doi.org/10.1126/science.272.5270.1910
  19. Huber KE, Waldor MK. Filamentous phage integration requires the host recombinases XerC and XerD. Nature. 2002;417(6889):656-659.  https://doi.org/10.1038/nature00782
  20. Waldor MK, Rubin EJ, Pearson GD, Kimsey H, Mekalanos JJ. Regulation, replication, and integration functions of the Vibrio cholerae CTXphi are encoded by region RS2. Molecular microbiology. 1997;24(5):917-926.  https://doi.org/10.1046/j.1365-2958.1997.3911758.x
  21. Das B, Nair GB, Bhadra RK. Acquisition and dissemination mechanisms of CTXΦ in Vibrio cholerae: New paradigm for dif residents. World J Med Genet. 2014; 4(2): 27-33.  https://doi.org/10.5496/wjmg.v4.i2.27
  22. Faruque SM, Asadulghani A, Kamruzzaman M, Nandi RK, Ghosh AN, Nair GB., et al. RS1 element of Vibrio cholerae can propagate horizontally as a filamentous phage exploiting the morphogenesis genes of CTXphi. Infection and immunity. 2002;70(1):163-170.  https://doi.org/10.1128/IAI.70.1.163-170.2002
  23. Davis BM., Kimsey HH, Kane AV, Waldor MK. A satellite phage-encoded antirepressor induces repressor aggregation and cholera toxin gene transfer. The EMBO journal. 2002;21(16):4240-4249. https://doi.org/10.1093/emboj/cdf427
  24. Hassan F, Kamruzzaman M, Mekalanos JJ., Faruque SM. Satellite phage TLCφ enables toxigenic conversion by CTX phage through dif site alteration. Nature. 2010;467(7318):982-985.  https://doi.org/10.1038/nature09469
  25. Olsvik O, Wahlberg J, Petterson B, Uhlén M, Popovic T, Wachsmuth IK, et al. Use of automated sequencing of polymerase chain reaction-generated amplicons to identify three types of cholera toxin subunit B in Vibrio cholerae O1 strains. Journal of clinical microbiology. 1993;31(1):22-25.  https://doi.org/10.1128/jcm.31.1.22-25.1993
  26. Mekalanos JJ. Duplication and amplification of toxin genes in Vibrio cholerae. Cell. 1983;35(1):253-263.  https://doi.org/10.1016/0092-8674(83)90228-3
  27. Goodarzi NN, Badmasti F, Jouriani FH, Fereshteh S. High-throughput genomic and proteomic interpretation of gene duplication in Vibrio cholera genomes: An in silico study Informatics in Medicine Unlocked. Informatics in Medicine Unlocked. 2023(3):e101262  https://doi.org/10.1016/j.imu.2023.101262
  28. Davis B.M., Moyer K.E., Boyd E.F., Waldor M.K. CTX prophages in classical biotype Vibrio cholerae: functional phage genes but dysfunctional phage genomes. Journal of bacteriology. 2000;182(24):6992-6998. https://doi.org/10.1128/JB.182.24.6992-6998.2000
  29. Karaolis DK, Johnson JA, Bailey CC, Boedeker EC, Kaper JB, Reeves PR. A Vibrio cholerae pathogenicity island associated with epidemic and pandemic strains. Proc Natl Acad Sci USA. 1998;95(6):3134-3139. https://doi.org/10.1073/pnas.95.6.3134
  30. Beck NA, Krukonis ES, DiRita VJ. TcpH influences virulence gene expression in Vibrio cholerae by inhibiting degradation of the transcription activator TcpP. Journal of bacteriology. 2004;186(24):8309-8316. https://doi.org/10.1128/JB.186.24.8309-8316.2004
  31. Prouty MG, Osorio CR, Klose KE. Characterization of functional domains of the Vibrio cholerae virulence regulator ToxT. Molecular microbiology. 2005;58(4):1143-1156  https://doi.org/10.1111/j.1365-2958.2005.04897.x
  32. Matson JS, Withey JH, DiRita VJ. Regulatory networks controlling Vibrio cholerae virulence gene expression. Infect Immun. 2007;75(12):5542-5549. https://doi.org/10.1128/IAI.01094-07
  33. Boyd EF., Waldor MK. Evolutionary and functional analyses of variants of the toxin-coregulated pilus protein TcpA from toxigenic Vibrio cholerae non-O1/non-O139 serogroup isolates. Microbiology. 2002;148(6):1655-1666. https://doi.org/10.1099/00221287-148-6-1655
  34. Sarkar A, Nandy RK, Nair GB, Ghose AC. Vibrio pathogenicity island and cholera toxin genetic element-associated virulence genes and their expression in non-O1 non-O139 strains of Vibrio cholerae. Infection and immunity, 2002;70(8):4735-4742. https://doi.org/10.1128/IAI.70.8.4735-4742.2002
  35. Nandi B, Nandy RK, Vicente AC, Ghose AC. Molecular characterization of a new variant of toxin-coregulated pilus protein (TcpA) in a toxigenic non-O1/Non-O139 strain of Vibrio cholerae. Infection and immunity. 2000;68(2):948-952.  https://doi.org/10.1128/IAI.68.2.948-952.2000
  36. Kirn T, Jude B, Taylor R. A colonization factor links Vibrio cholerae environmental survival and human infection. Nature. 2005;(438):863-866.  https://doi.org/10.1038/nature04249
  37. Jermyn WS, Boyd EF. Characterization of a novel Vibrio pathogenicity island (VPI-2) encoding neuraminidase (nanH) among toxigenic Vibrio cholerae isolates. Microbiology. 2002;148(11):3681-3693. https://doi.org/10.1099/00221287-148-11-3681
  38. Jermyn WS, Boyd EF. Molecular evolution of Vibrio pathogenicity island-2 (VPI-2): mosaic structure among Vibrio cholerae and Vibrio mimicus natural isolates. Microbiology. 2005;151(1):311-322.  https://doi.org/10.1099/mic.0.27621-0
  39. Taviani E, Grim CJ, Choi J, Chun J, Haley B, Hasan NA, et al. Discovery of novel Vibrio cholerae VSP-II genomic islands using comparative genomic analysis. FEMS microbiology letters. 2010;308(2):130-137.  https://doi.org/10.1111/j.1574-6968.2010.02008.x
  40. O’Shea YA, Finnan S, Reen FJ, Morrissey JP, O’Gara F, Boyd EF. The Vibrio seventh pandemic island-II is a 26.9 kb genomic island present in Vibrio cholerae El Tor and O139 serogroup isolates that shows homology to a 43.4 kb genomic island in V. vulnificus. Microbiology. 2004;150(12):4053-4063. https://doi.org/10.1099/mic.0.27172-0
  41. Waldor MK, Tschäpe H, Mekalanos JJ. A new type of conjugative transposon encodes resistance to sulfamethoxazole, trimethoprim, and streptomycin in Vibrio cholerae O139. Journal of bacteriology. 1996;178(14):4157-4165. https://doi.org/10.1128/jb.178.14.4157-4165.1996
  42. Spagnoletti M, Ceccarelli D, Rieux A, Fondi M, Taviani E, Fani R, et al. Acquisition and evolution of SXT-R391 integrative conjugative elements in the seventh-pandemic Vibrio cholerae lineage. mBio. 2014;5(4):e01356-14.  https://doi.org/10.1128/mBio.01356-14
  43. Wozniak RA, Fouts DE, Spagnoletti M, Colombo MM, Ceccarelli D, Garriss G, et al. Comparative ICE genomics: insights into the evolution of the SXT/R391 family of ICEs. PLoS genetics. 2009;5(12):e1000786. https://doi.org/10.1371/journal.pgen.1000786
  44. Hochhut B, Waldor MK. Site-specific integration of the conjugal Vibrio cholerae SXT element into prfC. Molecular microbiology. 1999;32(1):99-110.  https://doi.org/10.1046/j.1365-2958.1999.01330.x
  45. Beaber JW, Hochhut B, Waldor MK. Genomic and functional analyses of SXT, an integrating antibiotic resistance gene transfer element derived from Vibrio cholerae. Journal of bacteriology. 2002;184(15):4259-4269. https://doi.org/10.1128/JB.184.15.4259-4269.2002
  46. Smirnova NI, Rybal’chenko DA, Shchelkanova EYu, Lozovsky YuV, Krasnov YaM, Kutyrev VV. Variability of multiple resistance to antibiotics in cholera agent associated with different types of SXT element and spontaneous chromosome mutations. Molecular Genetics, Microbiology and Virology. 2022;40(2):28-36. (In Russ.). https://doi.org/10.17116/molgen20224002128
  47. Weill F, Domman D, Njamkepo E, Tarr C, Rauzier J, Fawal N, et al. Genomic history of the seventh pandemic of cholera in Africa. Science. 2017;358(6364):785-789.  https://doi.org/10.1126/science.aad5901
  48. Safa A, Nair GB, Kong RY. Evolution of new variants of Vibrio cholerae O1. Trends Microbiol. 2010;18(1):46-54.  https://doi.org/10.1016/j.tim.2009.10.003
  49. Nair GB, Faruque SM, Bhuiyan NA, Kamruzzaman M, Siddique AK, Sack DA. New variants of Vibrio cholerae O1 biotype El Tor with attributes of the classical biotype from hospitalized patients with acute diarrhea in Bangladesh. J. Clin. Microbiol. 2002;40(9):3296-3299. https://doi.org/10.1128/JCM.40.9.3296-3299.2002
  50. Ильина Т.С. Системы адаптивного иммунитета бактерий: связь с самосинтезирующимися транспозонами, полифункциональность. Молекулярная генетика, микробиология и вирусология. 2022;40(3): 13-21.117-126. 
  51. Ilyina T.S. Adaptive immunity system of bacteria: association with self-synthesizing transposons, polyfunctionality. Molecular Genetics, Microbiology and Virology. 2022.37(3), 13-21. https://doi.org/10.17116/molgen20224003113
  52. van der Oost J., Jore MM, Westra ER, Lundgren M, Brouns SJ. CRISPR-based adaptive and heritable immunity in prokaryots. Trends Biochem.Sci.2009,34(8).400-407. https://https://doi.org/10.1016/j.tibs.2009.05.002
  53. Naha A, Mandal RS, Samanta P, Saha RN, Shaw S, Ghosh A, et al. Deciphering the possible role of ctxB7 allele on higher production of cholera toxin by Haitian variant Vibrio cholerae O1. PLoS neglected tropical diseases. 2020;14(4):e0008128. https://doi.org/10.1371/journal.pntd.0008128
  54. Ghosh P, Sinha R, Samanta P, Saha DR, Koley H, Dutta S, et al. Haitian variant Vibrio cholerae O1 strains manifest higher virulence in animal models. Frontiers in microbiology. 2019;(10):111.  https://doi.org/10.3389/fmicb.2019.00111
  55. Ramamurthy T, Mutreja A, Weill FX, Das B, Ghosh A, Nair GB. Corrigendum: revisiting the global epidemiology of cholera in conjunction with the genomics of Vibrio cholerae. Frontiers in public health. 2019;(7):237.  https://doi.org/10.3389/fpubh.2019.00237
  56. Talkington D, Bopp C, Tarr C, Parsons MB, Dahourou G, Freeman M, et al. Characterization of toxigenic Vibrio cholerae from Haiti, 2010-2011. Emerging infectious diseases. 2011;17(11):2122-2129. https://doi.org/10.3201/eid1711.110805
  57. Chapman CML, Kapinos A, Rivera-Chávez F. Modulation of Host-Microbe Metabolism by Cholera Toxin. Infect Immun. 2023;91(5):e0043522. https://doi.org/10.1128/iai.00435-22
  58. Murphy SG, Johnson BA, Ledoux CM, Dörr T. Vibrio cholerae’s mysterious Seventh Pandemic island (VSP-II) encodes novel Zur-regulated zinc starvation genes involved in chemotaxis and cell congregation. PLoS genetics. 2021;17(6):e1009624. https://doi.org/10.1371/journal.pgen.1009624
  59. Smirnova NI, Rybal’chenko DA, Plekhanov NA, Lozovsky YuV, Fedorov AV, Kutyrev VV. New genetic variants of cholera agent and their distribution in epidemic countries and Russia. Molecular Genetics, Microbiology and Virology. 2023;41(1):10-17. (In Russ.). https://doi.org/10.17116/molgen20234101110
  60. Dolores J, Satchell KJ. Analysis of Vibrio cholerae genome sequences reveals unique rtxA variants in environmental strains and an rtxA-null mutation in recent altered El Tor isolates. mBio. 2013;4(2):e00624. https://doi.org/10.1128/mBio.00624-12
  61. Monakhova EV, Ghosh A, Mutreja A, Weill F, Ramamurthy T. Endemic Cholera in India and Imported Cholera in Russia: What is Common? Problems of Particularly Dangerous Infections. 2020;(3):17-26.  https://doi.org/10.21055/0370-1069-2020-3-17-26
  62. Kim HB, Wang M, Ahmed S, Park CH, LaRocque RC, Faruque AS, et al. Transferable quinolone resistance in Vibrio cholerae. Antimicrobial agents and chemotherapy. 2010;54(2):799-803.  https://doi.org/10.1128/AAC.01045-09
  63. Samanta P, Mandal RS, Saha RN, Shaw S, Ghosh P, Dutta S, et al. A point mutation in carR is involved in the emergence of polymyxin B-sensitive Vibrio cholerae O1 El Tor biotype by influencing gene transcription. Infection and immunity. 2020;88(5):e00080-20.  https://doi.org/10.1128/IAI.00080-20.
  64. Monir MM, Islam MT, Mazumder R, Mondal D, Nahar KS, Sultana M, et al. Genomic attributes of Vibrio cholerae O1 responsible for 2022 massive cholera outbreak in Bangladesh. Nat Commun. 2023 Mar 1;14(1):1154. https://doi.org/10.1038/s41467-023-36687-7
  65. Maciel-Guerra A, Babaarslan K, Baker M, Rahman A, Hossain M, Sadique A, et al. Core and accessory genomic traits of Vibrio cholerae O1 drive lineage transmission and disease severity. Nat Commun. 2024 Sep 23; 15(1):8231. https://doi.org/10.1038/s41467-024-52238-0
  66. Смирнова Н.И., Агафонов Д.А., Щелканова Е.Ю., Заднова С.П., Черкасов А.В., Кутырев В.В. Геноварианты возбудителя холеры Эль Тор: получение, молекулярно-генетический и протеомный анализ. Мол. генет., микробиол. и вирусол. 2014; 1: 21-30. 
  67. Smirnova N.I., Agafonov D.A., Shchelkanova E.Yu., Zadnova S.P., Cherkasov A.V., Kutyrev V.V. Genovariants of the cholera agent biovar El Tor: construction, molecular-genetic and proteomic analysis. Mol. Genet., Microbiol., Virol. 2014. 42(1): 34-42. https://doi.org/10.3103/S0891416814010042
  68. Rivera-Chávez F, Mekalanos JJ. Cholera toxin promotes pathogen acquisition of host-derived nutrients. Nature. 2019;572(7768):244-248.  https://doi.org/10.1038/s41586-019-1453-3
  69. Teschler JK, Zamorano-Sánchez D, Utada AS, et al. Living in the matrix: assembly and control of Vibrio cholerae biofilms. Nat Rev Microbiol. 2015;13(5):255-268.  https://doi.org/10.1038/nrmicro3433
  70. Смирнова Н.И., Агафонов Д.А., Кульшань Т.А., Щелканова ЕЮ, Краснов Я.М., Лозовский ЮВ. и др. Влияние делеции профага CTXφ возбудителя холеры на экспрессию регуляторных генов, контролирующих вирулентность и образование биопленки. Генетика. 2017; 53(2):1-14. 
  71. Smirnova NI, Agafonov DA, Kul’shan’ TA. Shchelkanova EYu, Krasnov YaM, Lozovsky YuV, et al. Effect of CTXφ prophage deletion in cholera agent on expression of regulatory genes controlling virulence and biofilm formation. Russian Journal of Genetics. 2017; 53(2):1-14 (In Russ.). https://doi.org/10.1134/S1022795417020119
  72. Hsiao A, Zhu J. Pathogenicity and virulence regulation of Vibrio cholerae at the interface of host-gut microbiome interactions. Virulence. 2020; 11(1):1582-1599. https://doi.org/10.1080/21505594.2020.1845039
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